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Application and preparation method of Beauveria sp extract having tyrosinase inhibition activity

A technology of tyrosinase and Beauveria bassiana, applied in the direction of microorganism-based methods, biochemical equipment and methods, organic active ingredients, etc., can solve the problems that Beauveria bassiana has no medicinal reports, etc., and achieve the prevention and treatment of pigmentation and the use of Extensive and preventive effect on melanoma

Inactive Publication Date: 2014-04-30
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main active ingredient of Beauveria bassiana is considered to be ammonium oxalate, but there is no medicinal report of Beauveria bassiana itself

Method used

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  • Application and preparation method of Beauveria sp extract having tyrosinase inhibition activity
  • Application and preparation method of Beauveria sp extract having tyrosinase inhibition activity
  • Application and preparation method of Beauveria sp extract having tyrosinase inhibition activity

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] The extract with tyrosinase inhibitory activity in Beauveria bassiana is extracted from the fermentation product of Beauveria bassiana, and the specific extraction steps are as follows:

[0063] 1. Selection and cultivation of Beauveria bassiana strains

[0064] The Beauveria bassiana used was Beauveria bassiana;

[0065] The specific cultivation method is as follows:

[0066] 1.1 Slant culture

[0067] The Beauveria bassiana strain was inoculated on SDAY slant medium, kept at a constant temperature of 20°C, and cultured for 4 days to obtain the first-grade strain;

[0068] 1.2 Secondary strain culture

[0069] Inoculate the primary strains into the liquid shake flask culture medium,

[0070] Liquid shake flask medium: glucose 40g / L, peptone 10g / L, yeast extract powder 10g / L, distilled water to volume;

[0071] Add 30% of the volume of the Erlenmeyer flask into a liquid culture medium larger than 100ml, inoculate each Erlenmeyer flask with 1 slant strain, place it ...

Embodiment 2

[0100] The extract with tyrosinase inhibitory activity in Beauveria bassiana is extracted from the fermentation product of Beauveria bassiana, and the specific extraction steps are as follows:

[0101] 1. Selection and cultivation of Beauveria bassiana strains

[0102] The Beauveria bassiana used was Beauveria brongniartii;

[0103] The specific cultivation method is as follows:

[0104] 1.1 Slant culture

[0105] The Beauveria bassiana strain was inoculated on the SDAY slant medium, kept at a constant temperature of 30°C, and cultured for 7 days to obtain the first-class strain;

[0106] 1.2 Secondary strain culture

[0107] Inoculate the primary strains into the liquid shake flask culture medium,

[0108] Liquid shake flask medium: glucose 40g / L, peptone 10g / L, yeast extract powder 10g / L, distilled water to volume;

[0109] Add 50% of the volume of the liquid medium in the 1000ml Erlenmeyer flask, inoculate each Erlenmeyer flask with 3 slant strains, place the inoculati...

Embodiment 3

[0139] The extract with tyrosinase inhibitory activity in Beauveria bassiana is extracted from the fermentation product of Beauveria bassiana, and the specific extraction steps are as follows:

[0140] 1. Selection and cultivation of Beauveria bassiana strains

[0141] The Beauveria bassiana used is Beauveria amorpha;

[0142] The specific cultivation method is as follows:

[0143] 1.1 Slant culture

[0144] The Beauveria bassiana strain was inoculated on SDAY slant medium, kept at a constant temperature of 25°C, and cultivated for 5 days to obtain the first-grade strain;

[0145] 1.2 Secondary strain culture

[0146] Inoculate the primary strains into the liquid shake flask culture medium,

[0147] Liquid shake flask medium: glucose 40g / L, peptone 10g / L, yeast extract powder 10g / L, distilled water to volume;

[0148] Add 40% of the volume of the Erlenmeyer flask into a 250ml Erlenmeyer flask, inoculate each Erlenmeyer flask with 1 slant strain, place it in a full-tempera...

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Abstract

The invention relates to an application and a preparation method of a Beauveria sp extract having tyrosinase inhibition activity, specifically to an application of the Beauveria sp extract in preparation of pigmentation and melanoma prevention and treatment tyrosinase inhibitors. According to the present invention, the main effective component in the Beauveria sp extract is 6'-methyl-2',4'-dihydroxyphenyl-4-O-methyl-beta-D-glucopyranoside, and the content is 5-99%; the activity is characterized in that the half-inhibition concentration (IC50) on the tyrosinase is 37.6-638.5 mug / mL; and the Beauveria sp extract having the tyrosinase inhibitor has wide uses, can be adopted as the daily chemical additive, and provides effects of whitening, anti-blackening, anti-aging, pigmentation and melanoma prevention and treatment, and the like.

Description

technical field [0001] The invention belongs to the technical field of extraction, preparation and application of biological products, and relates to the application and preparation method of a Beauveria bassiana extract with activity of inhibiting tyrosinase. Background technique [0002] Classification of Beauveria sp.: fungi of the genus Beauveria Vuillemin in the family Hyphomycetales; there are three common types of this genus in my country, such as: Beauveria bassiana ), Beauveria brongniartii and Beauveria amorpha are important entomopathogenic fungi, currently mainly used for biological control of pests. Wherein the silkworm is called Beauveria bassiana after being infected with Beauveria bassiana. The role of white silkworm in traditional Chinese medicine in my country is mainly "expelling wind and relieving spasm, resolving phlegm and resolving stagnation". The main active ingredient of Beauveria bassiana is considered to be ammonium oxalate, but there is no medic...

Claims

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Application Information

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IPC IPC(8): A61K8/60A61K31/7048A61Q19/02A61Q19/08A61P17/00A61P35/00C12P19/44C12R1/645
Inventor 陆瑞利胡丰林黄勃李增智
Owner ANHUI AGRICULTURAL UNIVERSITY
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