Delivery of viral agents

A disease and plant material technology, applied in the direction of viruses/phages, biocides, medical raw materials derived from viruses/phages, etc., can solve problems such as germination rate, growth and/or yield reduction, and adverse effects of factors on plant growth

Active Publication Date: 2014-04-23
NEXABIOME LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] Bacterial infection of plants can lead to reduced germination, growth and / or yield, respectively
It is desirable to treat or prevent those infections, but the methods currently used run the risk of adversely affecting another factor that affects plant growth

Method used

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  • Delivery of viral agents
  • Delivery of viral agents
  • Delivery of viral agents

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0107] Example 1 - Efficiency of LVCCs in delivering phage against deep wound infection.

[0108] figure 1 Schematically shown is the pressing of the inventive LVCC ( 1 ) onto a wound ( 4 ) in tissue ( 3 ) through a dressing ( 2 ).

[0109] Figure 2a is an inverted dressing of the invention incorporating an LVCC, shown in cross-section. The bag (5) encloses the LVCC (6) adhered to the dressing (7), which has a dressing top surface (8) - thus, it is inverted in the figure.

[0110] Figure 2b A LVCC (9) woven into a dressing (10) is schematically shown. The two filaments (9a and 9b) are different colors and have different phage attached. image 3 A further schematic of the dressing (11) incorporating phage-attached filaments (12) is shown.

[0111] In a first example, an in vivo model was used to demonstrate the efficiency of LVCCs in delivering phage against bacterial infection in the setting of an infected deep wound. Deep wounds penetrating multiple tissue layers inf...

Embodiment 2

[0115] Example 2 - Prolonged Stability

[0116] LVCCs recovered from infected subjects were washed and stored at 4°C and periodically assessed for their in vitro sustained antimicrobial activity against MRSA growth.

[0117] In a series of experiments, LVCC was demonstrated to maintain anti-MRSA activity by placing it in a bacterial lawn. After storage, phage activity (as shown by the cleared areas seen around the recovered LVCC) persists for several weeks -- see Figure 5 .

Embodiment 3

[0118] Embodiment 3--LVCC resists the stability brought by drying, ultraviolet radiation and temperature

[0119] In these examples, activated nylon polymers or cotton fibers were used as carrier matrices to create LVCCs. Phage-attached LVCCs were exposed to a range of environmental conditions, including desiccation, as indicated by the effect of relative humidity on phage survival (see Figure 6a ), UV light (see Figure 6b ) and elevated temperature (see Figure 6c ), all conditions are known to denature free phage.

[0120] Three 2×1 cm phage strips were placed on LBM agar and exposed to ultraviolet light for 5, 15 and 30 min in a sterile operating cabinet. The strips were then turned over and placed on a fresh area of ​​the agar, and the UV exposure was repeated. Control phage strips were treated similarly but shielded from UV light. Then, these strips were transferred to 15 ml of LB liquid medium, inoculated with 50 μl of Staphylococcus aureus suspension and cultured...

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PUM

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Abstract

The present invention discloses a plant material to which bacteriophage has been attached, wherein the bacteriophage retains infectivity. The plant material includes fruits, vegetables, leaves, stems, flowers, roots, tubers, seedlings and seeds. Plant diseases and animal diseases can be treated. A separate composition comprises a carrier selected from (i) a filament, (ii) a planar material, and (iii) particles and / or beads, and bacteriophage covalently attached thereto, wherein the bacteriophage retains infectivity, and is useful in treatment or prevention of bacterial infection in a deep wound.

Description

technical field [0001] The present invention relates to a method of delivering viral agents, such as bacteriophages or other viruses, in a sustainable viable state and in a manner suitable for the prevention and control of microbial infection, colonization and / or contamination. Also provided is a system as a method to locate and deliver genetically modified viable viruses adapted to deliver specific genes / proteins for therapy or immunization. The present invention can be used to combat the growth and / or colonization of microorganisms / bacteria in almost any situation where contamination, colonization or infection by microorganisms / bacteria exists or can occur, and where viruses such as bacteriophages are maintained The antibacterial activity is favorable. Background technique [0002] Today, it is generally accepted that contamination or infection by microorganisms / bacteria is a major contributor to affecting normal biological / physiological function (eg, healing or growth) a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/76A61K47/46A61L15/36A01N63/40A61K35/768
CPCA01N63/00A23K1/14A23K1/009A01N37/46A61L15/36A61L15/44A61K35/768A61K35/76A61K47/48776A23K1/00A61L2300/442A23K1/182A61L2300/404C12N2795/00032A23K10/10A23K10/18A23K10/30A23K50/70A23K50/75A61P1/00A61P31/04A01N63/40
Inventor J·查德威克M·马泰
Owner NEXABIOME LTD
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