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Ganoderma applanatum polysaccharide preparation method

A technology of Ganoderma lucidum and Ganoderma lucidum powder, applied in fermentation and other directions, can solve the problem of high cost

Active Publication Date: 2014-04-23
天津中益达生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, the cultivation of ganoderma ganoderma polysaccharides is mainly based on a solid medium based on carbon source glucose, but the cost is relatively high in large-scale industrial production

Method used

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  • Ganoderma applanatum polysaccharide preparation method
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  • Ganoderma applanatum polysaccharide preparation method

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preparation example Construction

[0027] A kind of preparation method of ganoderma ganoderma polysaccharide, the innovation of the present invention is: comprise the following steps:

[0028] (1) Uniformly mix the Ganoderma lucidum powder with the solid medium;

[0029] (2) Cultivate the product of step (1) at a constant temperature of 35-37 degrees Celsius for 24-48 hours;

[0030] (3) Centrifuge the fermented mycelium and wash it with distilled water for 2 to 3 times;

[0031] (4) Put the cleaned mycelium into a drying box, and dry it at 45-60 degrees Celsius to obtain the finished product.

[0032] Wherein, 150-200 grams of ganoderma ganoderma powder is added to each liter of the solid medium.

[0033] Each liter of said solid medium includes:

[0034] Mushroom bran 200-400 grams

[0035] Glucose 5-15 grams

[0036] Sodium dihydrogen phosphate 0.2~1.0 g

[0037] Potassium chloride 0.5~1.0 g

[0038] Vitamin B1 5~10 ml

[0039] Peptone 5-8 grams

[0040] The balance is water

[0041] After each com...

Embodiment 1

[0043] (1) Evenly mix 150 grams of Ganoderma lucidum powder with 1 liter of solid medium;

[0044] Solid media are:

[0045] Shiitake mushroom chaff 200 g

[0046] Glucose 5 g

[0047] Sodium dihydrogen phosphate 0.2 g

[0048] Potassium chloride 0.5 g

[0049] Vitamin B1 5 ml

[0050] Peptone 5 g

[0051] The balance is water

[0052] After each component is blended, it is autoclaved for 30 minutes under the condition of 0.1MPa.

[0053] (2) Cultivate the product of step (1) at a constant temperature of 35 degrees Celsius for 24 hours;

[0054] (3) Centrifuge the fermented mycelium at 800-1500 rpm, and wash it with distilled water for 3 times;

[0055] (4) Put the cleaned mycelium into a drying box, and dry it at 45 degrees Celsius to obtain a finished product.

[0056]

Embodiment 2

[0058] (1) Evenly mix 200 grams of Ganoderma lucidum powder with 1 liter of solid medium;

[0059] Solid media are:

[0060] Shiitake mushroom chaff 400 g

[0061] Glucose 15 g

[0062] Monosodium Phosphate 1.0 g

[0063] Potassium chloride 1.0 g

[0064] Vitamin B1 10ml

[0065] Peptone 8 g

[0066] The balance is water

[0067] After each component is blended, it is autoclaved for 30 minutes under the condition of 0.1MPa.

[0068] (2) Cultivate the product of step (1) at a constant temperature of 35 degrees Celsius for 24 hours;

[0069] (3) Centrifuge the fermented mycelium at 800-1500 rpm, and wash it twice with distilled water;

[0070] (4) Put the cleaned mycelium into a drying box, and dry it at 45 degrees Celsius to obtain a finished product.

[0071]

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Abstract

The invention relates to a ganoderma applanatum polysaccharide preparation method, which comprises: blending ganoderma applanatum powder and a solid culture medium, carrying out constant temperature fermentation, carrying out centrifugation separation to obtain mycelium, washing to remove the culture solution adhered on the mycelium surface, and drying to obtain the finished product. According to the present invention, the solid culture medium adopts mushroom bran as the main component to replace part of the carbon source glucose, then is mixed with the other components, and then is subjected to high pressure sterilization so as to be used, the fermentation effect is good, the ganoderma applanatum polysaccharide yield is increased by 3-12%, the ganoderma applanatum polysaccharide purity is increased by 2-5%, the cost is reduced by 20%, and the preparation method is suitable for large scale industrial production.

Description

technical field [0001] The invention belongs to the technical field of Ganoderma lucidum polysaccharide extraction, in particular to a preparation method of Ganoderma lucidum polysaccharide. Background technique [0002] Ganoderma lucidum, also known as ganoderma lucidum, old mother fungus or maple fungus, is born on dead standing logs and felling piles of broad-leaved trees such as poplar, birch, willow and oak. The fruiting bodies are large or extra large, sessile or basic Sessile, cap semicircular, oblate hemispherical or flat, base often decelerating, surface gray, gradually brown. Ganoderma lucidum contains polysaccharides, steroidal compounds, triterpenes, esters, amino acids, polypeptides, proteins, alkaloids, phenols, lactones, coumarins and trace elements and other nutrients. It can dispel wind and dampness when used medicinally , clearing heat and relieving pain, dissolving accumulation, hemostasis, and resolving phlegm, folks are mostly used to treat acute and ch...

Claims

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Application Information

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IPC IPC(8): C12P19/04
Inventor 刘鼎阔王立红赵云英赵晶晶张俊霞
Owner 天津中益达生物技术有限公司
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