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Application of hydrogen sulfide and donor thereof in improving sperm quality and function

A sperm quality and hydrogen sulfide technology, applied in the field of reproductive applications, can solve problems such as onset and unpredictability, and achieve the effects of reducing stress damage, maintaining quality, and improving spermatogenesis disorders.

Active Publication Date: 2014-04-23
昭远制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

While some studies have shown that certain treatments are effective, no single treatment works for all patients and it is impossible to predict which patients it will work for

Method used

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  • Application of hydrogen sulfide and donor thereof in improving sperm quality and function
  • Application of hydrogen sulfide and donor thereof in improving sperm quality and function
  • Application of hydrogen sulfide and donor thereof in improving sperm quality and function

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: hydrogen sulfide (H 2 S) Improvement of sperm motility in isolated semen from patients with asthenozoospermia.

[0033] Semen samples from patients with asthenozoospermia were selected for the experiment. Semen ejaculation by masturbation, using a specific sterile container to collect sperm samples after 2-7 days of abstinence. The sample water bath was kept at 22-37°C, and the liquefied semen was evaluated according to the fifth edition ("WHO5") of the WHO "Laboratory Manual for the Examination and Processing of Human Semen" ("WHO5"), using a computer-assisted semen analyzer CASA (IVOS; Hamilton-Thorn Research, Inc. Beverly, MA, USA), sperm parameters (volume, sperm count, percentage of motility and motility properties) were assessed.

[0034] The hydrogen sulfide donor GYY4137 was dissolved in normal saline and added to the semen at a final concentration of 2.5 μM. Semen was placed at 37°C, 5% CO 2 The sperm motility was evaluated by computer-aided s...

Embodiment 2

[0036] Embodiment 2: hydrogen sulfide (H 2 S) Improvement of sperm motility in isolated spermatozoa subjected to oxidative stress in sperm culture medium

[0037] Sperm samples from healthy male sperm donors are selected for the experiment. Semen ejaculation by masturbation, using a specific sterile container to collect sperm samples after 2-7 days of abstinence. The sample water bath was kept at 22-37°C, and the liquefied semen was evaluated according to the fifth edition ("WHO5") of the WHO "Laboratory Manual for the Examination and Processing of Human Semen" ("WHO5"), using a computer-assisted semen analyzer CASA (IVOS; Hamilton-Thorn Research, Inc. Beverly, MA, USA), sperm parameters (volume, sperm count, percentage of motility and motility properties) were assessed.

[0038] Density gradient centrifugation was used to separate sperm from semen, and the separated sperm were cultured in commercial sperm culture medium, first incubated with 50 μM hydrogen sulfide donor GYY...

Embodiment 3

[0040] Embodiment 3: hydrogen sulfide (H 2 S) Protective effect on LPS-induced spermatogenesis disorder in mice

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Abstract

The invention discloses an application of hydrogen sulfide and donor thereof in improving the sperm quality and function, and relates to the field of reproduction. The hydrogen sulfide can be directly added into semen in vitro, and the activity of the sperms of clinical asthenospermia patients can be remarkably improved within short time. The hydrogen sulfide also can resist oxidative stress for the sperms cultured in vitro so as to reduce the stress injury of the sperms caused by manual operation and in-vitro culture and maintain the activity level of the sperms. The hydrogen sulfide also can be used for improving the dyszoospermia caused by infection, protecting the spermatogenic structure of testis, maintaining the completion of blood-testis barrier and the synthesis of androgen, reducing the inflammatory level in the testis tissue and the oxidative stress of spermatogenic cells, and reducing the apoptosis of spermatogenic cells caused by infection so as to maintain a normal spermatogenic process, keep the sperm quality and maintain normal fertility of male. The invention opens up a new application field of hydrogen sulfide and provides a significant reference to solving the human dysgenesis and improving the assisted reproductive technology.

Description

technical field [0001] The present invention relates to a kind of gas molecule hydrogen sulfide (Hydrogen sulfide, H 2 S) and the use of its donors, especially in the field of reproduction. Background technique [0002] According to the report of the World Health Organization, in the world, about 5%-30% of married couples are infertile, and the proportion of infertility is more prominent in developing countries. The incidence rate in my country is about 10%, and there is a gradual increase Upward trend. Among them, male infertility is an important factor causing human reproductive disorders, accounting for about 40%-60%. In recent years, the advent of assisted reproductive technologies, such as artificial insemination (IUI), in vitro fertilization-embryo transfer (IVF) and intracytoplasmic sperm injection (ICSI), has provided a powerful means to solve human reproductive disorders. However, whether it is the maintenance of normal reproductive function or the treatment of as...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/04A61P15/08
Inventor 季勇王婧孟国梁肖玉娇谢利平韩艺李双越
Owner 昭远制药有限公司
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