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Method for preparing lucid ganoderma polysaccharides

The technology of Ganoderma lucidum polysaccharide and ultrasonic wave is applied in the preparation field of Ganoderma lucidum polysaccharide, which can solve the problems of polysaccharide biological activity and its structural influence, difficulty in product separation, high production cost, and achieve lustrous flavor and active ingredients, simple extraction process and drying speed. quick effect

Active Publication Date: 2014-04-02
福建省神六保健食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the need to add enzymes in the extraction process, not only the production cost is high, but also the separation of the product is difficult.
Solvent extraction methods mostly use acidic or alkaline substances, which may have a certain impact on the biological activity and structure of polysaccharides
In addition, there are also research reports on the combined use of ultrasonic wall breaking and hot water extraction to extract polysaccharides from Ganoderma lucidum. Since the ultrasonic wave is used to crush Ganoderma lucidum first, and then the polysaccharides are extracted in a constant temperature water bath, although the crushing efficiency of Ganoderma lucidum can be improved to a certain extent, the processing capacity is relatively small. , the extraction time is long, and it is impossible to really go out of the laboratory for industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A preparation method of Ganoderma lucidum polysaccharide, the steps of which are: cutting Ganoderma lucidum into pieces, crushing, suspending 10 kg of Ganoderma lucidum in 100 L of pure water, and extracting by overflowing and circulating ultrasonic crushing under the condition of 60°C, the working frequency of ultrasonic energy gathering is 15 KHz, the ultrasonic power is 60 W, and the total crushing time is 10 min. Among them, the ultrasonic working time is 20 s, the intermittent time is 20 s, and the treatment is repeated in a cycle; the extract is centrifuged at 6000 r / min at a high speed for 120 min at 90 °C, Remove the sediment, separate the supernatant through the ultrafiltration membrane, collect the active polysaccharide ultrafiltrate part; use the circulating foam to separate the active polysaccharide ultrafiltrate part, adjust the pH to 4.0, and introduce air to remove the foam to achieve the removal The purpose of the protein in the solution: the Ganoderma lu...

Embodiment 2

[0026] A preparation method of Ganoderma lucidum polysaccharide, the steps of which are: cutting Ganoderma lucidum into pieces, crushing, taking 10 kg of Ganoderma lucidum and suspending it in 100 L of pure water, and extracting it by overflowing and circulating ultrasonic crushing under the condition of 80°C, and the working frequency of ultrasonic energy gathering is 33 KHz, ultrasonic power was 4600 W, and the total crushing time was 90 min, among which, the working time of ultrasonic wave was 15 s, the intermittent time was 15 s, and the treatment was repeated repeatedly; the extract was centrifuged at 10000 r / min for 90 min, remove the sediment, separate the supernatant through the ultrafiltration membrane, and collect the active polysaccharide ultrafiltrate part; use the circulating foam to separate the active polysaccharide ultrafiltrate part, adjust the pH to 5.0, and introduce air to remove the foam. To achieve the purpose of removing the protein in the solution; the G...

Embodiment 3

[0028] A preparation method of Ganoderma lucidum polysaccharide, the steps of which are: cutting Ganoderma lucidum into pieces, crushing, suspending 10 kg of Ganoderma lucidum in 100 L of pure water, and extracting by overflowing and circulating ultrasonic crushing under the condition of 90°C, and the working frequency of ultrasonic energy gathering is 80 KHz, the ultrasonic power was 24000 W, and the total crushing time was 120 min, among which, the ultrasonic working time was 5 s, the intermittent time was 20 s, and the treatment was repeated repeatedly; the extract was centrifuged at 18000 r / min at a high speed for 10 min, remove the sediment, separate the supernatant through the ultrafiltration membrane, and collect the active polysaccharide ultrafiltrate part; use the circulating foam to separate the active polysaccharide ultrafiltrate part, adjust the pH to 6.0, and introduce air to remove the foam to To achieve the purpose of removing the protein in the solution; the Gan...

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Abstract

The invention discloses a method for preparing lucid ganoderma polysaccharides. The method comprises the following steps: using lucid ganoderma as a raw material; after overflow circulation ultrasonic shattering and extracting, separating a supernatant through high-speed centrifuging, removing proteins from the supernatant through circular current foam separation, and carrying out microwave vacuum drying to obtain lucid ganoderma polysaccharide powder. Due to the adoption of overflow circulation ultrasonic shattering and extracting, the shattering efficiency of the lucid ganoderma can be increased, the extracting rate of the lucid ganoderma active polysaccharides is increased, and automation operation can be conveniently carried out. The preparing method has the advantages of simplicity, low energy consumption, short time and capability of well preserving the natural activity of the lucid ganoderma polysaccharides and is suitable for industrial production. The obtained lucid ganoderma polysaccharides can be used for preparing different types of food, health food or drugs.

Description

technical field [0001] The invention belongs to the field of biochemical separation and purification, and in particular relates to a preparation method of ganoderma lucidum polysaccharide. Background technique [0002] Ganoderma lucidum is a treasure in the treasure house of traditional Chinese medicine in my country. It has the effects of preventing and curing diseases and prolonging life. It is known as "immortal grass". The "Shen Nong's Materia Medica" in the Eastern Han Dynasty and the "Compendium of Materia Medica" by Li Shizhen, a famous medical scientist in the Ming Dynasty, all have detailed records on the efficacy of Ganoderma lucidum. Today, Ganoderma lucidum has been officially recorded in the National Pharmacopoeia as a medicine. At the same time, it is a new resource food approved by the state. It has no toxic side effects and can be used for both medicine and food. [0003] Modern pharmacology and clinical practice have further confirmed that Ganoderma lucidum...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 谢金鉴骆建华谢新辞
Owner 福建省神六保健食品有限公司
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