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Extraction method and application of safflower polypeptide

An extraction method and safflower technology, which are applied in the field of safflower polypeptide extraction, can solve problems such as no safflower polypeptide extraction method, and achieve the effects of rapid absorption, high purity and simple operation.

Pending Publication Date: 2021-08-24
新疆沐禾生物科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there is no report on the extraction method of safflower polypeptide

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A method for extracting safflower polypeptides, the method for extracting safflower polypeptides comprises the following steps:

[0022] S1 After cleaning and drying the safflower seeds, grind the safflower powder to obtain the safflower powder, add NaOH solution to the safflower powder until the pH value is 8.5, extract at a temperature of 35°C for 1.5h, and then centrifuge to remove the safflower powder. insoluble matter, to obtain safflower protein extract;

[0023] S2 Adjust the pH value of the safflower protein extract obtained in step S1 to 7, add enzymatic protease for enzymolysis at a temperature of 45°C to obtain an enzymatic solution, the enzymatic protease is alkaline protease and flavor Protease, the mass ratio of alkaline protease and flavor protease is 1: 1;

[0024] S3 Boil the enzymatic solution obtained in step S2 to inactivate the enzyme, the time for boiling to inactivate the enzyme is 15 minutes, centrifuge after cooling, and take the supernatant, w...

Embodiment 2

[0028] S1 After cleaning and drying the safflower seeds, grind them to obtain safflower powder, add NaOH solution to the safflower powder until the pH value is 8.5, extract at a temperature of 40°C for 1.5h, and then centrifuge to remove the safflower powder. insoluble matter, to obtain safflower protein extract;

[0029] S2 Adjust the pH value of the safflower protein extract obtained in step S1 to 6.5, add enzymatic protease for enzymolysis at a temperature of 45°C to obtain an enzymatic hydrolysis solution, the enzymatic protease is neutral protease and flavor Protease, the mass ratio of neutral protease and flavor protease is 1: 1;

[0030] S3: Boil the enzymatic hydrolyzate obtained in step S2 to inactivate the enzyme, the time for boiling to inactivate the enzyme is 18 minutes, centrifuge after cooling, and take the supernatant, which contains the safflower polypeptide;

[0031] In S4, the supernatant containing the safflower polypeptide obtained in step S3 is ultrafilt...

Embodiment 3

[0034] A method for extracting safflower polypeptides, the method for extracting safflower polypeptides comprises the following steps:

[0035] S1 After cleaning and drying the safflower seeds, grind them to obtain safflower powder, add NaOH solution to the safflower powder until the pH value is 9, extract at a temperature of 45°C for 1 hour, and then centrifuge to remove the insoluble to obtain the safflower protein extract;

[0036] S2 Adjust the pH value of the safflower protein extract obtained in step S1 to 6, and add enzymatic protease for enzymolysis at a temperature of 60° C. to obtain an enzymatic hydrolyzate, and the enzymatic protease is flavor protease;

[0037] S3: Boil the enzymatic hydrolyzate obtained in step S2 to inactivate the enzyme for 20 minutes, centrifuge after cooling, and take the supernatant, which contains the safflower polypeptide;

[0038] In S4, the supernatant containing the safflower polypeptide obtained in step S3 is ultrafiltered with a 500D...

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Abstract

The present invention discloses an extraction method and application of safflower polypeptide. The method comprises the following steps: grinding safflower seed kernels to obtain safflower powder, adding a NaOH solution into the safflower powder, extracting for 1-1.5 hours, then centrifuging to remove insoluble substances to obtain a safflower protein extracting solution, adjusting the pH value to 6-7, and adding enzymolysis protease at the temperature of 45-60 DEG C for enzymolysis to obtain an enzymolysis solution; and carrying out enzyme deactivation, cooling, centrifuging, taking the supernatant, carrying out ultrafiltration by using a filter membrane, carrying out glucose gel column separation, eluting by using an eluent, collecting the eluent, and filtering to obtain the safflower polypeptide extracting solution. According to the extraction method of the safflower polypeptide provided by the invention, a biological enzymolysis technology is adopted, safflower polysaccharide is successfully converted into active small molecule peptide, the small molecule peptide can be actively digested and utilized by a human body, and the absorption rate is high; and the safflower polypeptide extracted by the safflower polypeptide extraction method provided by the invention is lower than 500 Daltons and high in purity, the method is simple to operate and mild in extraction condition, and the obtained polypeptide can retain natural activity.

Description

technical field [0001] The invention relates to the technical field of natural product extraction, in particular to a safflower polypeptide extraction method and its application. Background technique [0002] Modern women are under tremendous work and life pressure, which can easily cause anxiety, restlessness, decreased sleep quality, increased blood viscosity, blockage of capillaries invisible to the naked eye, slowed blood flow, and deprived skin of oxygen. In severe cases, blood stasis will lead to pigmentation, and water will be squeezed out of blood vessels, which will cause the skin to lose its natural luster due to lack of oxygen, and pigmentation spots will appear on the skin surface. The use of skin care products containing peptides can stimulate the differentiation and regeneration of skin epidermal cells, strongly promote epidermal repair and healing, and inhibit the formation of scars. Skin care products containing peptides can provide nutrients to the dermis o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/14C07K1/34C07K1/16C07K1/36
CPCC12P21/06C07K1/145C07K1/34C07K1/16C07K1/36
Inventor 刘袭文
Owner 新疆沐禾生物科技有限责任公司
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