Preparation process of chondroitin sulfate chelated calcium with anti-osteoporosis function

Abstract

The invention belongs to the field of bioactive component extraction, and particularly relates to a preparation process of chondroitin sulfate chelated calcium with an anti-osteoporosis function. The method mainly comprises the following steps: S1, water covering and bone breaking; S2, a chelation reaction 1; S3, free calcium removal; S4, solid-liquid separation, S5, a chelation reaction 2, S6, enzymolysis, S7, free calcium removal, S8, feed liquid washing, S9, vacuum concentration, and S10, drying and packaging. The chelation reaction 1 comprises the following steps: under the conditions of 45-55 DEG C and normal pressure, using a strong reducing agent and a weak base, and under the condition of high-frequency ultrasonic treatment, enabling chondroitin sulfate molecules and calcium ions to be preliminarily combined, so as to achieve the effect of forming a partial chelation state. The invention discloses a special chelating treatment technology, so that chemical bonds between chondroitin sulfate molecules and calcium atoms are changed, stable coordinate bonds are generated, a product is converted into a chelating state from a non-chelating state, and the product has excellent effects in the aspects of calcium absorption and utilization and osteoporosis resistance.

Description

technical field [0001] The invention belongs to the field of bioactive component extraction, and in particular relates to a preparation process of chondroitin sulfate chelated calcium with anti-osteoporosis function. Background technique [0002] Chondroitin sulfate is a kind of glycosaminoglycan, which belongs to acidic mucopolysaccharide, and its molecular weight is generally 20-50KDa. It is widely used in the field of dietary supplements and has a significant effect on joint maintenance and elimination of inflammation. [0003] At present, the commercially available product of chondroitin sulfate is mainly chondroitin sulfate sodium salt (accounting for more than 95% of the market), and the dosage forms included in various national pharmacopoeias are chondroitin sulfate sodium. Sodium chondroitin sulfate only has a certain inhibitory effect on osteoarthritis, because most products only have sodium element (sodium content is about 7%). At the same time, after users take th...

AI Technical Summary

Problems solved by technology

[0005] Chondroitin sulfate calcium salt is a relatively new chondroitin raw material, but the calcium in the product has not been chelated in the currently retrieved preparation technologies (as follows Two patents), resulting in the calcium element not being "passivated", most of the chondroitin sulfate calcium is in a non-chelated state, and the calcium ion is in a free state in the product

Therefore, it is easy to lose in the process of absorption and transportation, and the calcium absorption rate decreases.

At the same time, chondroitin sulfate calcium in the chelated state has a special synergistic effect, and has the strengthening effect of anti-osteoarthritis and anti-osteoporosis, while unchelated calcium chondroitin sulfate does not have this effect

Again, the chondroitin sulfate calcium prepared by the prior art does not do deep impurity removal process, resulting in many residual impurities, such as sulfate, chloride, heavy metal residues, miscellaneous proteins and other impurities exceeding the standard, and the effective content of chondroitin sulfate is low

[0006] Chinese patent CN102816809 "a preparation process of chondroitin sulfate calcium salt", discloses a preparation process of chondroitin sulfate calcium salt, including adding water to cook, alkaline hydrolysis Extraction, enzymatic hydrolysis, adsorption and washing, decolorization, precipitation and drying and other process steps can be directly extracted from animal cartilage to prepare calcium chondroitin sulfate, but hydrogen peroxide needs to be added for decolorization in the process, and hydrogen peroxide will inevitably remain in the final product , there are food safety risks

At the same time, there is no special chelation treatment for the product in the process, so the chondroitin sulfate calcium in the product is in a non-chelated state

[0007] Chinese patent CN 105218703 A "a production process of chondroitin sulfate calcium", discloses a preparation process of chondroitin sulfate calcium, including calcium hydroxide solution soaking swelling Cartilage, enzymatic hydrolysis, ultrafiltration, precipitation, dehydration, drying and other process steps can extract chondroitin sulfate calcium from animal cartilage, but the patent does not consider comprehensive indicators for this product, such as product purity, impurity content, etc. , did not use a systematic method to comprehensively present product indicators, such as USP, CP and other international testing methods

[0008] Starting from the prior art, there is no special chelation step in the preparation process of the prepared chondroitin sulfate calcium, and there is no stable formation between chondroitin sulfate molecules and calcium ions. The chelated state (that is, the coordination bond), the calcium atom is in a free state in the product solution, and it is easy to lose from the molecule during the absorption and utilization of the product, and the absorption rate is low during the absorption process, and there is no cartilage sulfate chelation The unique synergistic effect of calcium does not have the effect of anti-osteoporosis

Images