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A kind of lactobacillus plantarum strain and application thereof

A technology of Lactobacillus plantarum and vegetable oil, applied in the field of microorganisms, can solve problems such as difficulties in microorganisms, achieve the effects of high use value, improve oil production efficiency, and reduce oil production cost

Active Publication Date: 2020-05-12
GUIZHOU EDUCATION UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, tea seed water slurry contains a large amount of tea saponin, and tea saponin has inhibitory effect on most microorganisms. Therefore, it is difficult for most microorganisms to reproduce and grow in tea seed water slurry. It is difficult to rapidly reproduce and grow microorganisms in the pulp, and there is no report related to the existence of microorganisms in tea seeds

Method used

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  • A kind of lactobacillus plantarum strain and application thereof
  • A kind of lactobacillus plantarum strain and application thereof
  • A kind of lactobacillus plantarum strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Embodiment 1: Screening and preservation of bacterial strains

[0067] Lactobacillus plantarum JJZ21 (or L.plantarum subsp.plantarum JJZ21) of the present invention is fermented from the self-made tea seed water slurry of Guizhou Teachers College "Key Laboratory of Development and Utilization of Guizhou Characteristic Biological Resources", No. 115, Gaoxin Road, Wudang District, Guiyang City, Guizhou Province The specific separation and identification steps are as follows:

[0068] 1. Obtaining the strain

[0069] Weigh 100g of tea seed kernels, add sufficient water and soak for 16 hours at 25°C, wash the soaked tea seeds, add water for 2 rounds of beating, and filter to obtain filtrate (that is, tea seed water slurry), and place the filtrate at 35°C Fermentation at constant temperature. After 5 hours, use the inoculation loop to dip the fermentation broth and spread it on the MRS plate for culture (25°C). Stain with methylene blue to observe whether it is a bacillus,...

Embodiment 2

[0083] Embodiment 2: Lactobacillus plantarum JJZ21 is applied to the production of vegetable oil

[0084] Weigh 1000g of Camellia oleifera seeds, add enough water to soak at 35°C for 16 hours, wash the soaked Camellia oleifera seeds, add water (tea seeds: water = 1:3) for 2 rounds of beating, and filter to obtain the filtrate. Ratio (tea seed yeast dry powder / filtrate: 1-5g / L) Add tea seed yeast (Meyerozyma caribbica JJZ11) to the filtrate, preserved in China Center for Typical Culture Collection (CCTCC), preservation number is CCTCC M 2016470; Chinese typical culture The preservation center and this laboratory can provide strains.) and Lactobacillus plantarum JJZ21 (the strains were activated in MRS liquid medium for 3 generations, and after adjusting the bacterial concentration to 3.0×109CFU / ml according to the McFarney turbidimetric method, press the fermentation broth 2% of the amount used for inoculation), stir evenly, put it in a container and place it in a constant temp...

Embodiment 4

[0090] Example 4: Lactobacillus plantarum JJZ21 is used to produce tea saponin.

[0091] Take tea seed saponin as an example. Weigh 1000g of tea seed kernels, add enough water to soak at 30°C for 16 hours, wash the soaked camellia seeds, add water (tea seeds: water = 1:3) for 2 rounds of beating, and filter to obtain the filtrate, which precipitates for 2.5 h, remove the precipitate, add tea seed yeast (Meyerozyma caribbica JJZ11) to the filtrate according to a certain ratio (tea seed yeast dry powder / filtrate: 1-5g / L), preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCCM 2016470; China Center for Type Culture Collection and our laboratory can provide strains), stir evenly, put it in a container and ferment at a constant temperature of 35°C for 5 hours, and then take out the floating matter on the upper layer. Then add plantarum lactobacillus JJZ21 in fermented liquid according to a certain ratio (bacterium classification is...

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Abstract

The invention discloses a Lactobacillus plantarum, which relates to the field of microorganisms. It is Lactobacillus plantarum JJZ21 (L.plantarum subsp.plantarum JJZ21), which was deposited in the China Center for Type Culture Collection (CCTCC) on September 9, 2016, with a deposit number of It is CCTCC M 2016471, and the preservation address is: Luojia Mountain, Bayi Road, Wuchang District, Wuhan City, Hubei Province, which is obtained by fermentation of tea seed kernel water slurry. The Lactobacillus plantarum provided by the present invention can be used for producing vegetable oil or producing tea saponin.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a strain of Lactobacillus plantarum subsp. plantarum JJZ21. Background technique [0002] Tea seed oil is an edible oil produced from the seeds of the tea tree (Camellia sinensis). It is known as oriental olive oil because it is rich in various bioactive components. At present, the production methods of tea seed oil officially reported mainly include cold pressing method, hot pressing method, aqueous enzymatic method, general solvent leaching method and carbon dioxide supercritical extraction method. These tea seed oil production processes all have some insurmountable problems. In order to find a way to solve the problem, Jiang Jinzhong recently reported a new tea seed oil production process - the tea seed oil bio-fermentation production process. The core of the process is to divide the tea seed water slurry into upper, middle and lower through biological fermentation. Th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P7/64C12P33/20C12R1/25
CPCC12P7/6445C12P33/20C12N1/205C12R2001/25
Inventor 姜金仲杨鹏鸣穆安德
Owner GUIZHOU EDUCATION UNIV
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