DNA (deoxyribonucleic acid) probe library for hybridization with EGFR (epidermal growth factor receptor) gene and method for enriching EGFR gene segments by use of same

Abstract

The invention provides a DNA (deoxyribonucleic acid) probe library for hybridization with an EGFR (epidermal growth factor receptor) gene. The DNA probe library comprises one or more DNA probes which can be hybridized with the EGFR gene, and the DNA probe comprises the following sequence: SEQ ID No.1, SEQ ID No.2, SEQ ID No.3, SEQ ID No.4, SEQ ID No.5, SEQ ID No.6, SEQ ID No.7, SEQ ID No.8, SEQ ID No.9 or SEQ ID No.10. The invention also provides a method for enriching the EGFR gene segments by use of the DNA probe library. On the basis, the invention also provides a method for detecting the gene structure mutation of the EGFR gene. By adopting the method provided by the invention, the EGFR gene segments can be enriched by thousands of and even tens of thousands of times, and the EGFR gene segments can be applied to the next-generation sequencing technology to detect the gene structure mutation, including single base mutation, mRNA (messenger ribonucleic acid) deletion or increase, mRNA structure transversion and mRNA splicing change.

Description

technical field [0001] The present invention relates to the field of gene detection. Specifically, the present invention relates to a method for enriching and extracting EGFR gene fragments. The method can accurately enrich EGFR gene fragments, and then selectively detect EGFR gene structural mutations. . Background technique [0002] DNA sequencing technology is undergoing drastic changes. Its outstanding feature is the observation and analysis of many sites at the same time (massively parallel), so as to gradually achieve a substantial increase in sequencing throughput. The cost of sequencing each base in the original data a sharp decline. Based on this, previously unattainable luxury activities (such as personal gene sequencing, metagenomics research) have gradually become more and more feasible. Especially with the development of science, because the traditional Sanger sequencing can no longer fully meet the needs of research, next-generation sequencing technology (Nex...

AI Technical Summary

Problems solved by technology

[0016] Aiming at the above-mentioned technical problems, the inventors have developed a method for capturing EGFR gene fragment sequences based on hybridization selection through a large number of experiments. By using this method, EGFR gene fragments enriched by thousands or even tens of thousands of times can be obtained. The collected EGFR gene fragment samples can be selectively applied to various gene detection technologies, especially the next generation sequencing technology can be applied to the detection of gene mutations, deletions, additions, and transversions

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