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Sea squirt polypeptide pharmaceutical composition as well as preparation method and application thereof

A sea squirt polypeptide and composition technology, applied in the field of sea squirt polypeptide pharmaceutical composition, can solve the problems such as weak effect of HBeAg, achieve the effect of inhibiting hepatitis B virus replication, high anti-hepatitis B virus activity, and improving pharmacological activity

Inactive Publication Date: 2014-03-26
天津拓飞科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Ascidia is a Uronordata subphylum Uronordata class. Hu Wenjun of the First Military Medical University published a dissertation titled "Studies on the Active Components of Ascidia Anti-Hepatitis B Virus" from two species isolated from Ascidia. The polypeptide has anti-hepatitis B virus activity. The amino acid sequences of these two polypeptides are Lys-Gly-Lys-Ile-Glu-His and Ser-Ser-Leu-Ser-Lys-Ala-Ala respectively. The negative conversion rate is high, but the effect on HBeAg is weak, and the effects of protecting the liver and reducing enzymes need to be further studied

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Prescription: Ascidian polypeptide 1mg, Phyllanthus urinaria alcohol extract 8g.

[0051] Preparation:

[0052] (1) The Phyllanthus urinaria was crushed into coarse powder, the percolation solution was extracted with 55% ethanol according to the percolation method to 8 times the amount of the medicinal material, filtered, the ethanol was recovered under reduced pressure and concentrated to 1:1 to obtain the alcoholic extract of Phyllanthus urinaria Things.

[0053] (2) Soak the ascidian with 95% ethanol equal to the weight of ascidian for 2 weeks, recover the ethanol to obtain the extract, dissolve the extract with 2 times the volume of water, then extract with the same volume of ethyl acetate as the water, take the water layer, and finally use Separation and purification by macroporous resin chromatography, take the eluent with an Rf of 0.5-0.6 for TLC UV detection, and evaporate the solvent to obtain ascidian polypeptide. The conditions of the thin-layer chromatography:...

Embodiment 2

[0056] Prescription: Ascidian polypeptide 3mg, Phyllanthus urinaria alcohol extract 7g.

[0057] (1) The Phyllanthus urinaria was crushed into coarse powder, the percolation liquid was extracted with 55% ethanol according to the percolation method to 7 times the amount of the medicinal material, filtered, the ethanol was recovered under reduced pressure and concentrated to 1:1 to obtain the alcoholic extraction of Phyllanthus urinaria Things.

[0058] (2) Soak the ascidian with 95% ethanol equal to the weight of ascidian for 2 weeks, recover the ethanol to obtain the extract, dissolve the extract with 3 times the volume of water, then extract with the same volume of water as chloroform, take the water layer, and finally use silica gel chromatography Method for separation and purification, take the eluent with an Rf of 0.5-0.6 detected by thin-layer chromatography, and evaporate the solvent to obtain ascidian polypeptide. The conditions of the thin layer chromatography: the develo...

Embodiment 3

[0061] Prescription: Ascidian polypeptide 6mg, Phyllanthus urinaria alcohol extract 7g.

[0062] Preparation:

[0063] (1) The Phyllanthus urinaria was crushed into coarse powder, and the percolation was extracted with 55% ethanol according to the percolation method to 6 times the amount of the medicinal material, filtered, the ethanol was recovered under reduced pressure and concentrated to 1:1 to obtain the alcoholic extract of Phyllanthus urinaria Things.

[0064] (2) Soak the ascidian with 95% ethanol equal to the weight of ascidian for 2 weeks, recover the ethanol to obtain the extract, dissolve the extract with 3 times the volume of water, then extract with the same volume of n-butanol with water, take the water layer, and finally Use silica gel chromatography and Sephadcx LH20 chromatography to separate and purify, take the eluent with Rf of 0.5-0.6 for TLC UV detection, and evaporate the solvent to obtain ascidian polypeptide. The conditions of thin-layer chromatography:...

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PUM

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Abstract

The invention discloses a sea squirt polypeptide pharmaceutical composition as well as a preparation method and application thereof. The composition comprises the following components in parts by weight: 0.001-0.01 parts of sea squirt polypeptide (the amino acid sequence is LysGlyLysIle Glu His) and 5-8 parts of phyllanthus urinaria alcohol extract. The invention further discloses application of the sea squirt polypeptide pharmaceutical composition in the preparation of medicine for treating hepatitis B. By adopting the sea squirt polypeptide pharmaceutical composition, relatively good drug synergism is played from pharmacological functions of multiple points, and the disadvantage of simple recipe is overcome.

Description

Technical field [0001] The invention relates to the field of antiviral drugs, in particular to an ascidian polypeptide pharmaceutical composition against hepatitis B virus. Background technique [0002] Hepatitis B is one of the diseases that seriously endanger the health of the people. At present, western medicines for hepatitis B virus (HBV) infections are mainly nucleoside drugs such as lamivudine and biological drugs such as interferon. These drugs have large side effects, are prone to drug resistance, and rebound after treatment. Disadvantages. [0003] Phyllanthus urinaria L. is the whole plant of Euphorbiaceae Phyllanthus phyllanthus. It was first collected in "The Essentials of Herbal Medicine". It has the effects of calming the liver and clearing heat, relieving toxins, and is used to treat enteritis and dysentery. , Urinary tract infection, unknown swelling and pain, etc. There are more than 600 species of Phyllanthus in the world. my country has 6 subgenres, 7 groups,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/08A61K47/46A61P1/16A61P31/20A61K36/47
Inventor 李虹
Owner 天津拓飞科技有限公司
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