Fungus capable of capturing conidium of mulberry powdery mildew

A technology of conidia and mulberry powder, applied in the direction of fungi, microorganism-based methods, chemicals for biological control, etc., to achieve the effects of easy preparation and production of bacterial agents, reduced production, and rapid growth

Inactive Publication Date: 2014-03-19
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Biological control method is a research hotspot in recent years. Biological control mainly uses antagonism, competition, or predation (parasitic or saprophytic) to prevent and control pathogenic bacteria. However, there are no relevant research reports at home and abroad on the use of microbial agents to prevent and control mulberry diseases.

Method used

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  • Fungus capable of capturing conidium of mulberry powdery mildew
  • Fungus capable of capturing conidium of mulberry powdery mildew
  • Fungus capable of capturing conidium of mulberry powdery mildew

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 pseudozyma aphidis Isolation and identification of CNm2012

[0021] 1. Isolation and purification of strains

[0022] (1) Separation. Medium: 200g potato, 20g glucose, 18g agar, 3g penicillin and streptomycin, 1L single distilled water. In the process of observing the germination of powdery mildew conidia, the strains found to have a predatory effect on it were spread on the medium and cultured at a constant temperature of 28°C.

[0023] (2) Purification. Sabouraud medium: glucose 40g, yeast extract 10g, peptone 10g, agar powder 36g, single distilled water 1000ml, pH 6.5-7.5. The culture was picked and streaked on the culture medium to inoculate the strain, and cultured at a constant temperature of 28°C.

[0024] (3) Conservation of species. Potato medium: 200g potato, 20g glucose, 18g agar, 1L single distilled water. The purified strains were kept on the slant of this medium.

[0025] 2. Morphological and molecular biological characteristics of th...

Embodiment 2

[0040] Example 2 strain pseudozyma aphidis Microscopic observation of the predation effect of CNm2012 on conidia of Powdery mildew Morus alba

[0041] Weigh 100g of potatoes, 10g of glucose, 9g of agar, 500ml of single distilled water, pH 6.5-7.5, put the prepared medium into a pressure cooker and sterilize at 120°C for 30 minutes. Strain CNm2012 was activated on the PDA plate. Then use the 500ml Erlenmeyer flask to ferment the bacterial strain. The used medium for fermentation is Sabouraud's medium, and its formula is: glucose 4g, yeast extract 1g, peptone 1g, single distilled water 100ml, PH6.5-7.5. The fermentation conditions are: temperature 20-30°C, 180r / min, fermentation for 4 days. The fermented bacterial liquid was centrifuged at 1000 rpm for 5 minutes to remove the supernatant, and then washed three times with sterile water by resuspending, centrifuging, and discarding the liquid. The thalli obtained in the above steps were mixed with powdery mildew conidia, re...

Embodiment 3

[0043] Example 3 Nutritional based on Powdery mildew pseudozyma aphidis Growth curve determination of CNm2012 strain

[0044] The CNm2012 bacterial strain is mixed with different concentrations of powdery mildew conidia respectively into a 100ml suspension, and the suspension contains a concentration of 10 5 cfu / ml of the CNm2012 strain, the concentrations were 6×10 2 cfu / ml, 6×10 3 cfu / ml, 6×10 4 cfu / ml of powdery mildew conidia. The mixture was cultured in a shaker at 28°C and 180r / min, and samples were taken every 12 hours to check its OD600 absorbance (each group used the conidia suspension of powdery mildew of Morus alba at its respective concentration as a blank control). Test Setup 10 5 The aqueous solution of CNm2012 at the concentration of cfu / ml was used as negative control. The results showed that the absorbance value of the CNm2012 strain increased with the increase of the conidia concentration of Powdery mildew (Table 1), indicating that pseudozym...

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Abstract

The invention discloses a fungus (pesudozyma aphidis CNm2012) capable of capturing conidium of mulberry powdery mildew, and the preservation number of the fungus is CCTCC No. M2013563. The bacterium strain is separated from mulberry leaf surface, is a yeast-like fungus, the color of the fungus colony is pink in a potato plate culture medium, smooth yeast-like colonies exist in the middle part, and hyphae grow on the edge; the bacterium strain is cultured in a Sabourand liquid culture medium for 7 days, the bacterial shape is like a shuttle in different sizes, many oil-drop-like substances exist in the bacterial cells, hyphae grow as the bacterium strains grow; the proper temperature for the growth of bacterium stain is 20 to 30 DEG C, the proper pH value is 6.5 to 7.5; the 18Sr strain's DNA sequence is 1416bp, and the NCBI serial number is KF443200; the 28Sr strain's DNA sequence is 627bp, and the serial number is KF443201; the ITSr strain's DNA sequence is 784bp, and the serial number is 443199. The fungus can capture and eat conidium of mulberry powdery mildew so as to carry out biological prevention on mulberry powdery mildew, and does not have any pathogenicity on mulberries or silkworms.

Description

technical field [0001] The invention belongs to the field of biological control of plant diseases, and in particular relates to a fungus capable of predating conidia of powdery mildew pseudozyma aphidis CNm2012. Background technique [0002] Mulberry is an important economic crop. In addition to its leaves that can be used to feed silkworms, its fruits are edible and used to extract anthocyanins, and its roots and stems can also be used as medicine. Mulberry powdery mildew is a disease of mulberry trees caused by Ascomycota subphylum Sclerotinia, Erysidiomycetes, Erysidiomycetes, and the fungus of the genus Epsilia. The damaged mulberry leaves have poor nutritional value and poor quality, so the silkworms who eat it become weaker. It is easy to induce silkworm disease, resulting in less cocoon volume and cocoon layer volume of silkworms. The outbreak of mulberry powdery mildew has caused huge economic losses to the entire sericulture industry, so how to prevent and cont...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01P3/00C12R1/645
Inventor 邱欣汪静杰张琳冯丽春段正巧刘艳萍陈倩茜万永继
Owner SOUTHWEST UNIVERSITY
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