Corydalis impatiens total alkaloid extractive for preparing anti-cancer drugs and application thereof

A technology of total alkaloids and anticancer drugs, which is applied in the application field of preparing anticancer drugs, can solve the problems of high impurity content, low product yield, and poor medicinal performance of total alkaloids, and achieve resource saving and simple operation Effect

Active Publication Date: 2014-03-12
SHANDONG JINHE DRUG RES DEV
View PDF5 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, the methods for extracting total alkaloids in plants are two kinds of organic solvent extraction and chromatography, and silica gel column chromatography, alumina column chromatography and ion exchange resin are commonly used in chromatography. Although the above methods can all Preparation of total alkaloids, but there are problems of low product yield and high impurity content, and the medicinal properties of the total alkaloids directly obtained by this method are slightly poor, which cannot meet clinical research and application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Corydalis impatiens total alkaloid extractive for preparing anti-cancer drugs and application thereof
  • Corydalis impatiens total alkaloid extractive for preparing anti-cancer drugs and application thereof
  • Corydalis impatiens total alkaloid extractive for preparing anti-cancer drugs and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0119] Take Corydalis sylvestris medicinal material and crush it into a coarse powder, pass through a 10-mesh sieve, add alkaline ethanol with a volume concentration of 50% saturation, and reflux extraction at 50°C for 3 times, each extraction for 3 hours, to obtain Corydalis sylvestris extract, of which this The amount of saturated alkaline ethanol used in the step is 10 times the mass of Corydalis serrata medicinal material; the alkaline ethanol is recovered under reduced pressure at 60°C for the above-mentioned Corydalis serrata extract, and then concentrated to a relative density of 1.1 (measured at 50°C). Paste, spray-dried to obtain the alkaline ethanol extract of Corydalis cypresse; adding hydrochloric acid with a volume concentration of 0.5% to the alkaline ethanol extract of Corydalis cypresse to dissolve and filter and repeat 3 times, wherein the amount of solid was added in each dissolution process The acid water liquid of 6 times of amount, merge the filtrate after ...

Embodiment 2

[0121] Take Corydalis sylvestris medicinal material and crush it into a coarse powder, pass through a 50-mesh sieve, add alkaline ethanol with a volume concentration of 95% saturation, and reflux extraction at 70°C for 3 times, each extraction for 2 hours, to obtain Corydalis sylvestris extract, of which this The amount of saturated alkaline ethanol used in the step is 8 times of the quality of Corydalis serrata medicinal material; the above-mentioned Corydalis serrata extract is recovered under reduced pressure at 60°C for alkaline ethanol, and then concentrated to a relative density of 1.05 (measured at 50°C). paste, spray-dried to obtain the alkaline ethanol extract of Corydalis serrata; adding hydrochloric acid with a volume concentration of 4% in the alkaline ethanol extract of Corydalis serrata to dissolve and filter and repeat 4 times, wherein adding solid mass of 1 times the amount of acid water liquid, the filtrate after merging and filtering forms acid water liquid, a...

Embodiment 3

[0123]Take Corydalis sylvestris medicinal material and crush it into coarse powder, pass through a 50-mesh sieve, add alkaline ethanol with a volume concentration of 90% saturation, and reflux extraction at 80°C for 2.5 hours each time to obtain Corydalis sylvestris extract, of which this The amount of saturated alkaline ethanol used in the step is 7 times of the quality of Corydalis sylvestris medicinal material; the above-mentioned Corydalis sylvestris extract is recovered under reduced pressure at 60°C for alkaline ethanol, and then concentrated to a relative density of 1.1 (measured at 50°C). Paste, spray-dried to obtain the alkaline ethanol extract of Corydalis sylvestris; adding sulfuric acid with a volume concentration of 1% to the alkaline ethanol extract of Corydalis sylvestris to dissolve and filter and repeat 3 times, wherein the solid amount and mass were added in each dissolution process The sulfuric acid solution of 3 times of amount, merge the filtrate after filt...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses corydalis impatiens total alkaloid extractive for preparing anti-cancer drugs. The content of total alkaloids in the discloses corydalis impatiens total alkaloid extractive is not less than 50wt percent; the disclosed corydalis impatiens total alkaloid extractive comprises 10 to 12wt percent of protopine, 8 to 10wt percent of alpha-bicuculline and 10 to 15wt percent of ochotensimine; the extracting steps of the extractive comprise grinding corydalis impatiens drugs, performing reflux extraction to the corydalis impatiens drugs through saturated alkaline ethyl alcohol or saturated alkaline methyl alcohol, and concentrating and trying the corydalis impatiens drugs, so as to obtain corydalis impatiens extractive, then dissolving and filtering the extractive through acid solution, adding alkali solution into filter liquor, and adjusting the pH value of the solution to be 9 to 10, so as to obtain acid and alkali treated fluid, adding organic solvent into the acid and alkali treated fluid, so as to perform extraction, obtaining corydalis impatiens total alkaloid crude extract, and then purifying the corydalis impatiens total alkaloid crude extract, so as to obtain the corydalis impatiens total alkaloid extractive. The corydalis impatiens total alkaloid extractive obtained through the method has effects of breast cancer resistance, ovarian cancer resistance, stomach cancer resistance and lung cancer resistance.

Description

technical field [0001] The invention relates to an application of an extract of a Chinese herbal medicine, in particular to an application of a total alkaloid extract of a Tibetan medical material Corydalis cypress for preparing anticancer drugs. Background technique [0002] The Tibetan medicine Corydalis Saibei is the dried whole herb of Corydalis saibei (also known as Corydalis saibei), which is a plant of the genus Corydalis Papadaceae. It is distributed in Qinghai, Tibet, Gansu, Sichuan and other places. It is a commonly used drug in Tibetan medicine. The data shows that 25% of Tibetan medicines contain this medicinal material, and the main ingredients in the medicinal material are various alkaloids. In Tibetan medicine, the whole plant of Corydalis genus is often used as medicine, and some of the roots, stems, leaves and other parts of the plant are used as medicine alone. It is mainly used to treat various bleeding symptoms, fever, flu, gastrointestinal and hepatobili...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K36/66A61P35/00A61K31/395A61K31/4741
Inventor 李怀平魏永义王海苹王朔
Owner SHANDONG JINHE DRUG RES DEV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap