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Traditional Chinese medicine composition for treating tumours and preparation method thereof

The technology of a composition and a traditional Chinese medicine is applied in the field of the composition containing the bufadenin extract and the preparation field thereof, and can solve the problems of not meeting the new drug application requirements of traditional Chinese medicine injection and the like

Active Publication Date: 2010-03-31
SHANGHAI CHENPON PHARM TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The total content of the three fat-soluble components reported in the existing literature is less than 80%, which does not meet the current new drug declaration requirements for traditional Chinese medicine injections

Method used

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  • Traditional Chinese medicine composition for treating tumours and preparation method thereof
  • Traditional Chinese medicine composition for treating tumours and preparation method thereof
  • Traditional Chinese medicine composition for treating tumours and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Take 1 gram of toad venom medicinal material, add 10 mL of 95% ethanol, heat to reflux for 40 min, and extract twice under the above conditions. The extract was filtered while it was hot, concentrated by rotary evaporation, and then 1 g of silica gel was added to mix the sample to obtain the mixed sample silica gel. Take 20 grams of silica gel soaked in petroleum ether: ethyl acetate (volume ratio 4.5:1) and wet-pack the column, add the sample silica gel, and use petroleum ether: ethyl acetate (volume ratio 4.5:1) for isocratic elute. Analysis by HPLC under normal conditions, such as figure 1 As shown, the retention time is 7.184min for bufalin, 8.886min for cinobufagin, and 9.549min for bufagenin. The eluate containing these three fat-soluble components is collected and evaporated to dryness, namely The bufagenin extract of the present invention is obtained, wherein the three active bufagenin extracts account for 97.88% by weight of the total extract.

Embodiment 2

[0020] Weigh 30mg of three kinds of bufagenin extracts, dissolve them in a 250mL brown volumetric flask with distilled water, ultrasonicate for 15min, place for 48h, filter with a 0.45μm filter membrane to obtain a transparent and clear medicinal solution, which is potted in 2mL The ampoule is sterilized by circulating steam to obtain a solution for injection with a filling volume of 2mL.

Embodiment 3

[0022] Weigh 30mg of three kinds of bufagenin extracts, dissolve them in a 250mL brown volumetric flask with distilled water, sonicate for 15min, place for 48h, filter with a 0.22μm filter membrane to obtain a transparent and clear medicinal solution, add appropriate amount of freeze-dried scaffold , sterilized, filled in a 10mL vial under aseptic conditions, and freeze-dried to obtain a freeze-dried powder for injection. The freeze-dried support agent is mannitol, lactose, or trehalose, or any of the three Mix in one proportion.

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Abstract

The invention discloses a traditional Chinese medicine composition for treating tumours and a preparation method thereof. The traditional Chinese medicine composition contains a bufogenin extract anda medicinal carrier, wherein the bufogenin extract is prepared by the purification of a silica gel column after being extracted by a toad venom medicinal material, the total content is not below 92%,and then the traditional Chinese medicine composition is prepared by dissolution, filtration, encapsulation and sterilization and can be further prepared into a liquor for injection or freeze-drying powder injection. The traditional Chinese medicine composition has a favorable anticancer action for lung cancer, liver cancer, mammary cancer, stomach cancer, colonic cancer and the like, can increaseleucocyte and has less side effect.

Description

technical field [0001] The invention relates to a traditional Chinese medicine composition and a preparation method thereof, in particular to a composition containing bufaxin extract for treating tumors and a preparation method thereof. Background technique [0002] Medicinal toad is Bufo bufogargarizans Cantor or Bufo melanostictus Schneider, an animal of the amphibian class Bufoidae. Made dry. Ancient and modern prescriptions widely use toads and toads to remove toxins and reduce swelling, relieve pain and kill insects, strengthen the heart and diuresis. Modern studies have shown that the mixture of three lipid-soluble ingredients, repobugenin, cinobufagin, and bufalin in Buad Suen not only has obvious anti-tumor effects, but also can transform tumor cells into normal cells (inducing tumor cell differentiation), induce tumor cell apoptosis, inhibit tumor cell angiogenesis, and reduce tumor metastasis. [0003] Traditional Chinese medicine injection is a new type of drug...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/56A61K31/585A61K9/08A61K9/19A61P35/00A61K35/65
Inventor 奉建芳杜狄峥何峻瑶
Owner SHANGHAI CHENPON PHARM TECH CO LTD
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