Traditional Chinese medicine composition for treating tumours and preparation method thereof
The technology of a composition and a traditional Chinese medicine is applied in the field of the composition containing the bufadenin extract and the preparation field thereof, and can solve the problems of not meeting the new drug application requirements of traditional Chinese medicine injection and the like
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Embodiment 1
[0018] Take 1 gram of toad venom medicinal material, add 10 mL of 95% ethanol, heat to reflux for 40 min, and extract twice under the above conditions. The extract was filtered while it was hot, concentrated by rotary evaporation, and then 1 g of silica gel was added to mix the sample to obtain the mixed sample silica gel. Take 20 grams of silica gel soaked in petroleum ether: ethyl acetate (volume ratio 4.5:1) and wet-pack the column, add the sample silica gel, and use petroleum ether: ethyl acetate (volume ratio 4.5:1) for isocratic elute. Analysis by HPLC under normal conditions, such as figure 1 As shown, the retention time is 7.184min for bufalin, 8.886min for cinobufagin, and 9.549min for bufagenin. The eluate containing these three fat-soluble components is collected and evaporated to dryness, namely The bufagenin extract of the present invention is obtained, wherein the three active bufagenin extracts account for 97.88% by weight of the total extract.
Embodiment 2
[0020] Weigh 30mg of three kinds of bufagenin extracts, dissolve them in a 250mL brown volumetric flask with distilled water, ultrasonicate for 15min, place for 48h, filter with a 0.45μm filter membrane to obtain a transparent and clear medicinal solution, which is potted in 2mL The ampoule is sterilized by circulating steam to obtain a solution for injection with a filling volume of 2mL.
Embodiment 3
[0022] Weigh 30mg of three kinds of bufagenin extracts, dissolve them in a 250mL brown volumetric flask with distilled water, sonicate for 15min, place for 48h, filter with a 0.22μm filter membrane to obtain a transparent and clear medicinal solution, add appropriate amount of freeze-dried scaffold , sterilized, filled in a 10mL vial under aseptic conditions, and freeze-dried to obtain a freeze-dried powder for injection. The freeze-dried support agent is mannitol, lactose, or trehalose, or any of the three Mix in one proportion.
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