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Nanorealgar-carrying magnetic albumin nanospheres and preparation method thereof

A technology of nano-albumin and nano-realgar, which is applied in pharmaceutical formulas, inorganic active ingredients, preparations for in vivo tests, etc., can solve problems affecting tumor imaging effects, reduce the enrichment of iron oxide nanoparticles, and achieve reliable experimental research Effects of Data and Theoretical Basis

Inactive Publication Date: 2014-03-05
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This will reduce the enrichment of iron oxide nanoparticles at the tumor site, thereby affecting the effect of tumor imaging

Method used

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  • Nanorealgar-carrying magnetic albumin nanospheres and preparation method thereof

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Embodiment 1

[0019] Embodiment 1: A preparation method of a drug-loaded magnetic albumin nanosphere that can be used as a magnetic resonance imaging contrast agent and can treat tumors according to the present invention, the specific steps are as follows:

[0020] 1) Preparation of Fe3O4 nanoparticles:

[0021] Preparation by chemical co-precipitation method: ① Weigh FeCl separately 3 ·6H 2 O and FeCl 2 4H 2 O 10.8 g and 4.37 g each, put into a jar, and dissolve in 200 ml triple distilled water; ② Pour 100 ml of 1 mol / L ammonia water into the jar quickly; ③ Put the liquid in a vent plug Put the jar on the magnetic stirrer, put the stirring bar, and fill it with N 2 Protection, set the reaction temperature to 50°C, set the stirring speed to 3000 rpm, and stop stirring after 30 min. Cool naturally, pour the liquid into a beaker, then use a strong magnet to hold the bottom of the beaker, pour off the upper layer of waste liquid, rinse the product with deionized water repeatedly, wash th...

Embodiment 2

[0028] Accurately weigh an appropriate amount of bovine serum albumin 400 mg, magnetic powder (Fe 3 o 4 Magnetic nanoparticles) 100 mg, add 500 ug nano-realgar and the above mixed solution dropwise, add triple distilled water until the total volume is 25 ml, at this time, the concentration of bovine serum albumin is 16 mg / ml, stir well and adjust the pH value For basicity, add absolute ethanol slowly under magnetic stirring until the solution appears cloudy, and then follow Fe 3 o 4 The ratio of magnetic nanoparticles to 2.5% glutaraldehyde aqueous solution 2000mg / ml is slowly added to glutaraldehyde for solidification, stirred for more than 8 hours, the reaction solution is poured into a centrifuge tube for ultra-high-speed centrifugation, and after washing with deionized water, the nano-loaded realgar is obtained. Magnetic albumin nanospheres.

Embodiment 3

[0030] Accurately weigh an appropriate amount of bovine serum albumin 600 mg, magnetic powder (Fe 3 o 4 Magnetic nanoparticles) 100 mg, add 500 ug nano-realgar and the above mixed solution dropwise, add three-distilled water and three-distilled water until the total volume is 25 ml, at this time, the concentration of bovine serum albumin is 24 mg / ml, stir and mix well , adjust the pH value to be alkaline, slowly add absolute ethanol under magnetic stirring until the solution appears cloudy, and then follow the Fe 3 o 4 The ratio of magnetic nanoparticles to 2.5% glutaraldehyde aqueous solution 2000mg / ml is slowly added to glutaraldehyde for solidification, stirred for more than 8 hours, the reaction solution is poured into a centrifuge tube for ultra-high-speed centrifugation, and after washing with deionized water, the nano-loaded realgar is obtained. Magnetic albumin nanospheres.

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Abstract

The invention discloses nanorealgar-carrying magnetic albumin nanospheres and a preparation method thereof. The method comprises the following steps of preparing iron oxide nanoparticles and nanorealgar by an improved chemical coprecipitation process; preparing nanorealgar-loaded magnetic albumin nanospheres by a desolvation-crosslinking process. The method is simple and easy to implement, integrates the advantages of thermal therapy, chemotherapy, thermal release control, magnetic targeting and the like through albumin nanospheres, and develops a new idea for tumor therapy. Apart from a therapy carrier, the novel nanocarrier also can be used for the in-vivo magnetic resonance imaging study to provide reliable experimental study data and theoretical basis to the clinical application and study.

Description

technical field [0001] The invention relates to a drug-loaded magnetic albumin nanosphere which can be used as a magnetic resonance imaging contrast agent and can treat tumors and a preparation method thereof. Background technique [0002] Realgar belongs to sulfide arsenic-containing mineral traditional Chinese medicine. It has significant curative effect on acute promyelocytic leukemia, chronic myeloid leukemia, multiple myeloma and other malignant hematological diseases. For some solid tumor cells, in vitro experiments have also confirmed that it can inhibit growth and induce Apoptosis, such as liver cancer cell lines, breast cancer multidrug-resistant cell lines, osteosarcoma cells, etc. Pang Qi et al. have found that realgar has a significant inhibitory effect on mouse glioma cells cultured in vitro, and in vivo experiments in animals have proved that local administration is effective. After 3 weeks of medication, 75% of the implanted tumors disappeared, and within 3 mo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/14A61K41/00A61K33/36A61K9/51A61K47/42A61P35/00A61P35/02
Inventor 张东生安艳丽唐秋莎
Owner SOUTHEAST UNIV
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