Preparation method of micropatterned nanofiber

Abstract

The invention relates to a preparation method of a micropatterned nanofiber. A physical structure of a salivary gland acinar cell system is simulated within nanometer and micrometer scale ranges by combining a photoetching micropattern array and utilizing the advantage that an electrostatic spinning PLGA (Polylactide / Glycolide) nanofiber membrane serves as an engineering salivary gland tissue; due to the addition of the photoetching micropattern array, the surface area of a nanofiber base material can be remarkably increased. The nanofiber prepared by the invention has more practical application values in the field of preparation of salivary glands for tissue engineering.

Description

technical field [0001] The invention relates to a method for preparing a PLGA nanofiber body, in particular to a method for preparing a micropatterned PLGA nanofiber body for salivary gland cell and tissue differentiation. Background technique [0002] Inability to produce enough saliva, or salivary gland dysfunction, is an important clinical problem and is the initial cause of xerostomia. Often caused by autoimmune disease, radiation therapy, or head injury, the disorder can reduce a person's ability to chew, swallow, digest, and speak, and in more severe cases can lead to oral infections, gum disease, and tooth decay. Treatment for salivary gland insufficiency usually involves the use of medications with many side effects or artificial saliva, which are temporary and inadequate. One possible approach for treating salivary gland dysfunction is to create engineered salivary glands that can permanently replace lost or damaged acinar tissue. [0003] Artificial salivary glan...

AI Technical Summary

Problems solved by technology

[0003] Artificial salivary glands mainly involve two methods of tissue engineering: one method is to self-assemble salivary gland epithelial cell lines or initial cells in a three-dimensional substrate, which can be artificial basement membrane or natural substrates such as hyaluronic acid, but the disadvantages are The closed spheres formed lack interconnectivity, which is not conducive to the capture of saliva, and artificial basement membranes cannot be transplanted into humans; the other is to use flat synthetic or natural substrates to organize salivary gland cells into a single layer However, salivary gland cells grown on flat polymeric substrates tend not to form the tight junctions necessary for directional secretion of saliva, nor do this method provide sufficient surface area and energy Three-dimensional dendrites that produce enough saliva