Preparation method of micropatterned nanofiber

A nanofiber and micropatterning technology, applied in the field of preparation of PLGA nanofibers, can solve the problems of unfavorable saliva capture, lack of interconnectivity of airtight spheres, and artificial basement membranes that cannot be transplanted into the human body. The effect of applying value

Inactive Publication Date: 2014-02-26
WUXI ZHONGKE GUANGYUAN BIOMATERIALS
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Problems solved by technology

[0003] Artificial salivary glands mainly involve two methods of tissue engineering: one method is to self-assemble salivary gland epithelial cell lines or initial cells in a three-dimensional substrate, which can be artificial basement membrane or natural substrates such as hyaluronic acid, but the disadvantages are The closed spheres formed lack interconnectivity, which is not conducive to the capture of saliva, and artificial basement membranes cannot be transplanted into humans; the other is to use flat synthetic or natural substrates to organize salivary gland cells into a single layer However, salivary gland cells grown on flat polymeric substrates tend not to form the tight junctions necessary for directional secretion of saliva, nor do this method provide sufficient surface area and energy Three-dimensional dendrites that produce enough saliva

Method used

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Embodiment Construction

[0009] In order to deepen understanding of the present invention, below in conjunction with specific example, the present invention is described in further detail:

[0010] (1) Preparation of micropatterned strip arrays: 200nm monocrystalline silicon wafers were washed with sulfuric acid and 30% hydrogen peroxide solution at a volume ratio of 3:1 for 2 minutes, then washed and dried, and then spin-coated with P20 at a speed of 2500rpm. Adhesion promoter, spin-coating time is 50s, positive photoresist SPR2207.0 is spin-coated at 1000rpm for 5min, then baked at 80°C for 10min; then exposed in a contact photolithography machine for 10min, soft The contact mode is less than 3mJ / cm 2 , after exposure, the resist was developed under AZ300MIF developer for 10 minutes under mild stirring conditions, and the temperature was raised to 160°C for 5 minutes. Thoroughly mixed with alkane, spin-coated onto the patterned silicon wafer at a spin-coating speed of 1000 rpm for 80 s, and then he...

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Abstract

The invention relates to a preparation method of a micropatterned nanofiber. A physical structure of a salivary gland acinar cell system is simulated within nanometer and micrometer scale ranges by combining a photoetching micropattern array and utilizing the advantage that an electrostatic spinning PLGA (Polylactide / Glycolide) nanofiber membrane serves as an engineering salivary gland tissue; due to the addition of the photoetching micropattern array, the surface area of a nanofiber base material can be remarkably increased. The nanofiber prepared by the invention has more practical application values in the field of preparation of salivary glands for tissue engineering.

Description

technical field [0001] The invention relates to a method for preparing a PLGA nanofiber body, in particular to a method for preparing a micropatterned PLGA nanofiber body for salivary gland cell and tissue differentiation. Background technique [0002] Inability to produce enough saliva, or salivary gland dysfunction, is an important clinical problem and is the initial cause of xerostomia. Often caused by autoimmune disease, radiation therapy, or head injury, the disorder can reduce a person's ability to chew, swallow, digest, and speak, and in more severe cases can lead to oral infections, gum disease, and tooth decay. Treatment for salivary gland insufficiency usually involves the use of medications with many side effects or artificial saliva, which are temporary and inadequate. One possible approach for treating salivary gland dysfunction is to create engineered salivary glands that can permanently replace lost or damaged acinar tissue. [0003] Artificial salivary glan...

Claims

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Application Information

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IPC IPC(8): A61L27/18A61L27/50D01D5/00
Inventor 韩志超许杉杉佴刚
Owner WUXI ZHONGKE GUANGYUAN BIOMATERIALS
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