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Preparation method of polylactic-co-glycolic acid (PLGA) nano-particles encapsulated with nano insulin

A technology of nanoparticles and insulin, applied in the field of preparation of PLGA nanoparticles, to achieve the effect of great application value

Inactive Publication Date: 2014-02-26
WUXI ZHONGKE GUANGYUAN BIOMATERIALS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In the application of nanotechnology, nanoparticles should be non-toxic to target organs or organic systems while focusing on enhancing bioavailability, but many nanomaterials are unable to do so

Method used

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Embodiment Construction

[0010] In order to deepen understanding of the present invention, below in conjunction with specific example, the present invention is described in further detail:

[0011] Preparation of blank comparison and drug-loaded nanoparticles: PLGA nanoparticles encapsulated with nano-insulin particles were prepared by solvent substitution technique, 90mg PLGA and 10mg dry bovine insulin were dissolved in 5mL acetone, 30mL containing stabilizer (1% polyethylene oxide The aqueous solution of polypropoxy) was added dropwise to the above-prepared organic phase solution at a rate of 1 mL / min, and the resulting mixture was stirred at room temperature until the organic solvent was completely evaporated; at 20°C, it was removed by centrifugation at a speed of 1000 rpm for 20 min. After removing the stabilizer, the obtained particles were redispersed into deionized water and washed three times, and the obtained suspended matter-loaded nanoparticles were stored at 20°C for future use. The same...

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Abstract

The invention relates to a preparation method of polylactic-co-glycolic acid (PLGA) nano-particles encapsulated with nano insulin. In L6 skeletal muscle cells, the product disclosed by the invention has good effects in the field of treatment of glucose absorption function damage caused by arsenic, insulin resistance and mitochondrial dysfunction, and different biomarkers, for example, pyruvate kinase, glucokinase, ATP / ADP ratio, a mitochondrial membrane, cytoplasm release of mitochondria cytochrome, cell membrane potential and calcium ion level indicate that the function of mitochondria is improved. The product disclosed by the invention has a great application prospect in treatment of diabetes mellitus caused by arsenic.

Description

technical field [0001] The present invention relates to a preparation method of polylactic acid copolyglycolic acid (PLGA) nanoparticles, in particular to a preparation method of PLGA nanoparticles encapsulated with nano-insulin for adjusting some important biomarkers in L6 cells with arsenic poisoning . Background technique [0002] Exposure to arsenic from polluted water is thought to be an important factor in many dire diseases. In areas with relatively high arsenic content, such as Taiwan and Bangladesh, the crisis of diabetes and its complications is gradually increasing, which is seriously threatening human health. The possible relationship between chronic arsenic exposure and diabetes is largely related to human health, so it has a very important guiding role in ensuring in-depth research. [0003] Generally speaking, impairment of glucose tolerance due to dysfunction of pancreatic β-cells is the main cause of diabetes. Diabetes can cause very serious symptoms, and ...

Claims

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Application Information

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IPC IPC(8): A61K9/14A61K38/28A61K47/34A61P39/02A61P3/10
Inventor 韩志超许杉杉佴刚
Owner WUXI ZHONGKE GUANGYUAN BIOMATERIALS
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