Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for efficiently extracting microbial oil

A microbial oil and microbial technology, applied in the direction of fat production, fat oil/fat production, etc., can solve the problems of low single extraction rate, large solvent consumption, low oil extraction rate, etc., and achieve high single extraction rate and solvent consumption The effect of a large amount and a small amount of solvent

Active Publication Date: 2015-09-30
WILMAR SHANGHAI BIOTECH RES & DEV CENT
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of this method are: 1. The microbial oil content in the water phase is low, and the solvent consumption in the extraction process is large; 2. The water phase system containing cell fragments has a high viscosity, and it is very easy to emulsify during the process of mixing with the solvent. Separation is more difficult; 3. The mass transfer efficiency of the aqueous phase and the solvent phase is low, and repeated extractions are often required to increase the recovery rate
The disadvantages of this method are: 1. The broken cell mixture in the aqueous phase system has a high viscosity and is easy to emulsify, so it is difficult to directly carry out centrifugation; 2. The viscosity of the mixture can be reduced by washing with water, but it is also easy to entrain microbial oil, and the oil extraction rate is low. Low; 3. Repeated washing will also produce a large amount of industrial sewage
In addition, 96% ethanol is not very soluble in oil and requires long-term extraction; the single extraction rate of ethanol extraction is very low, so the meal needs to be extracted repeatedly
Therefore, the method disclosed in this document has a low rate of oil recovery, requires repeated extractions, and takes a long time

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for efficiently extracting microbial oil
  • A method for efficiently extracting microbial oil
  • A method for efficiently extracting microbial oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] Embodiment 1: Add water to the influence of grease recovery rate

[0079] Mix 628g of algae powder (Schizochytrium sp. self-cultivated and spray-dried, water content 3.2%) with about 3L of n-hexane, and get 2638g of wall-broken mixture after treatment in a sand mill.

[0080] 1. Weigh 4 parts of the broken wall mixture, each about 20g;

[0081] 2. Add different proportions of water respectively and shake well;

[0082] 3. Centrifuge at 6000g for 5 minutes to separate the supernatant, which is mixed oil;

[0083] 4. The mixed oils were desolvated separately and weighed in an oven at 60°C to obtain hairy algal oil.

[0084] Calculate the recovery rate of algae oil, and the results are shown in Table 6.

[0085] Table 6. Algae oil recovery rate

[0086]

Embodiment 2

[0087] Embodiment 2: Add the influence of ethanol aqueous solution on oil recovery rate

[0088] Mix 628g of algae powder (Schizochytrium sp. self-cultivated and spray-dried, water content 3.2%) with about 3L of n-hexane, and get 2638g of wall-broken mixture after treatment in a sand mill.

[0089] 1. Weigh 4 parts of the broken wall mixture, each about 20g;

[0090] 2. Add different proportions of ethanol aqueous solution (the amount of water and ethanol added is shown in Table 7) and shake well;

[0091] 3. Centrifuge at 6000g for 5 minutes to separate the supernatant, which is mixed oil;

[0092] 4. The mixed oils were desolvated separately and weighed in an oven at 60°C to obtain hairy algal oil.

[0093] Calculate the recovery rate of algae oil, and the results are shown in Table 7.

[0094] Table 7. Algae oil recovery rate

[0095]

Embodiment 3

[0096] Embodiment 3: the influence of ethanol solution concentration on oil recovery rate

[0097] Mix 628g of algae powder (Schizochytrium sp. self-cultivated and spray-dried, water content 3.2%) with about 3L of n-hexane, and get 2638g of wall-broken mixture after treatment in a sand mill.

[0098] 1. Weigh 10 parts of the broken wall mixture, each about 20g;

[0099] 2. Add ethanol aqueous solution of different solubility respectively, and shake well, wherein the addition amount of ethanol aqueous solution is 2 times of the weight of algae cell in the broken wall mixture;

[0100] 3. Centrifuge at 2000g for 1min to separate the supernatant, which is mixed oil;

[0101] 4. The mixed oils were desolvated separately and weighed in an oven at 60°C to obtain hairy algal oil.

[0102] Calculate the algae oil recovery rate, and the results are shown in Table 8:

[0103] Table 8. Comparison of the effects of different concentrations of ethanol on the recovery of algae oil

...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for efficient extraction of microbial oil. Specifically, the method is as below: mixing an oil extraction solvent with microbes, conducting wall breaking, adding water or an aqueous solution of low-carbon chain alcohol, so as to simply and efficiently extract oil.

Description

technical field [0001] The invention belongs to the field of microbial oil extraction, and in particular relates to a method for extracting microbial oil. Background technique [0002] The microbial cells harvested by cultivation generally exist in the aqueous phase system, with fine particles, high oil content, and tough cell walls. Therefore, the traditional vegetable oil extraction process is not suitable for the preparation of microbial oil. The preparation of general microbial oil includes 4 steps: 1. Cultivate the microorganisms in a fermenter using an appropriate medium; 2. Harvest the biomass of the microorganisms and process them (such as washing / dehydrating or drying); 3. Cell wall breaking, Including physical treatment (cooking, steam explosion), chemical (hot alkali, chelating agent), mechanical (pressing, homogenization, grinding) and other means; 4. Extracting microbial oil from cell debris (such as solvent extraction, direct centrifugation). [0003] US 0061...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C11B1/02C11B1/10
Inventor 洪丰王勇姜元荣邹滢张敏
Owner WILMAR SHANGHAI BIOTECH RES & DEV CENT
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com