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Nano-particle wrapped with small RNA (Ribose Nucleic Acid) for inhibiting embryo implantation

A nanoparticle and tiny technology, applied in the field of molecular cytology, can solve the problems of increased risk of thrombosis and undiscovered miRNA, and achieves the effect of simple preparation method, obvious contraceptive effect and good stability

Inactive Publication Date: 2014-02-12
ANHUI RUBIOX VISION BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Using artificially synthesized estrogen and progesterone to achieve contraception, the objective problem is that it can only suppress ovulation or change the viscosity of cervical fluid to achieve contraceptive effect through hormones, and changing hormones will cause certain risks. e.g. increased risk of blood clots, breast cancer, etc.
During the process of embryo implantation, the levels of many miRNAs change, but no specific miRNA has been found to regulate embryo implantation

Method used

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  • Nano-particle wrapped with small RNA (Ribose Nucleic Acid) for inhibiting embryo implantation
  • Nano-particle wrapped with small RNA (Ribose Nucleic Acid) for inhibiting embryo implantation
  • Nano-particle wrapped with small RNA (Ribose Nucleic Acid) for inhibiting embryo implantation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Nanomaterial polyethylene glycol-polylactic acid encapsulated small molecule mir-181

[0019] PEG 5000- PLA 25000 Dissolve BHEM-Chol in 0.5mL chloroform, add the miRNA solution, and form an initial emulsion under ultrasonication with a probe-type ultrasonic breaker (output power 80 watts, ultrasonication at a frequency of 10 seconds and 2 seconds for 2 minutes), and add the initial emulsion to into 1.5mL of 1% (w / v) polyvinyl alcohol aqueous solution, and ultrasonically (same as above) emulsified again, the emulsion was added to 25mL of 0.3% (w / v) PVA aqueous solution, the organic solvent was evaporated under reduced pressure, centrifuged, The nanoparticles were collected, resuspended twice with ultrapure water, washed by centrifugation, and the collected samples were freeze-dried.

[0020] The hydrophilic segment PEG of polyethylene glycol-polylactic acid copolymer can improve the compatibility and dispersion of the carrier and the drug, and the hydrophobic...

Embodiment 2

[0022] Example 2 Stability monitoring of nanoparticle miRNA

[0023] After injecting miRNA into mice by intraperitoneal injection, anesthesia gas was used to keep mice under anesthesia during the detection process, and a small animal in vivo imager was used to observe the fluorescence intensity in mice to monitor the stability of miRNA. The red fluorescent marker cy5 is bound to the miRNA used, and the fluorescence intensity directly reflects the stability of the miRNA. Once the small RNA is degraded, the fluorescence of cy5 disappears; in a large number of experiments, we have confirmed that the activity is basically the same as not being degraded. like figure 1 As shown, the real-time monitoring of the stability of miRNA over time shows that miRNA encapsulated by nanomaterials can exist in vivo for more than 72 hours, indicating that nanomaterial encapsulation can significantly delay the degradation rate of miRNA in vivo.

Embodiment 3

[0024] Inhibitory effect of embodiment 3mir-181a and mir-181b on embryo implantation

[0025] The mice were injected with negative RNA, mir-181a or mir-181b particles wrapped in nanomaterials on the 2nd day of pregnancy, and injected from the intraperitoneal cavity for 3 days, once a day (to achieve the highest level of miRNA maintenance in the body), each injection The amount of the drug is 1-2OD, the injection concentration is 5μM, and the injection volume is 500μL. Then, the number of embryos in the mouse uterus was detected at the 7th day of pregnancy, and it was found that the embryos could not implant and form embryos after injection of mir-181a and mir-181b, indicating that mir-181a and mir-181b encapsulated by nanoparticles can significantly inhibit Embryo implantation.

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Abstract

The invention discloses a nano-particle wrapped with small RNA (Ribose Nucleic Acid) for inhibiting embryo implantation, and belongs to the technical field of molecular cytology. The inhibiting effect of mir-181 to LIF (Leukemia Inhibitory Factor) protein expression is firstly determined, and the inhibiting effect of the same to embryo implantation is verified; furthermore, nanometer material polyethylene glycol-polyethylene glycol is prepared to be nano-particles wrapped with the small RNA, and the nano-particles are good in stability in mice and successful to inhibit embryo implantation. The preparation method provided by the invention is simple; contraceptive product obtained by the method inhibits embryo implantation in a non-hormonal manner to achieve the effect of contraception, thus being free from side effect to human body caused by the change of hormone, good in stability in vivo and obvious in contraceptive effect.

Description

technical field [0001] The invention relates to a nanoparticle coated with microRNA that inhibits embryo implantation, especially a nanoparticle coated with microRNA mir-181 that inhibits embryo implantation by inhibiting the expression of LIF protein, and belongs to the technical field of molecular cytology . Background technique [0002] Using artificially synthesized estrogen and progesterone to achieve contraception, the objective problem is that it can only suppress ovulation or change the viscosity of cervical fluid to achieve contraceptive effect through hormones, and changing hormones will cause certain risks. Such as increased risk of blood clots, breast cancer, etc. Using small molecule RNA technology to specifically realize the promotion of embryo implantation by inhibiting LIF protein, so as to achieve contraception and solve the side effects of hormonal contraceptives on the body. [0003] The LIF protein is a cytokine that inhibits cell differentiation and pr...

Claims

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Application Information

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IPC IPC(8): C12N15/113A61K48/00A61K47/34A61K9/19A61P15/18
Inventor 吴缅初波汪国兴
Owner ANHUI RUBIOX VISION BIOTECH
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