Fungi medium

Abstract

The invention relates to a fungi medium which comprises the following raw materials: 15-18 g/L potato extract powder, 3-5 g/L yeast extract powder, 20-22 g/L glucose and 15-20 g/L agar powder. The fungi medium prepared by taking the potato extract powder with stable quality and the yeast extract powder which enriches a fungi growth environment and is convenient for fungi metabolism observation, as raw materials is stable in quality, simple in material preparation, convenient in material getting, easy to prepare and good in operability; when the fungi medium is used for fungi culture, fungi cells with good growth synchronicity, thick and uniform fungi layers and high spore productivity can be obtained and the culture cycle of fungi can be shortened to be only 3-5 days.

Description

technical field [0001] The invention belongs to the technical field of microbial engineering, and in particular relates to a mold culture medium. Background technique [0002] In the prior art, the medium used for cultivating molds is usually potato medium (referred to as PDA medium). The potato medium is prepared by the following method: take fresh potatoes, wash and peel them, and prepare to cut them into pieces according to the prepared volume. For small pieces of potatoes, boil them with water for 30 minutes and filter them with degreasing gauze. Add sucrose in the volume of preparation to the filtrate, make up water according to the volume of preparation, put them into inclined bottles filled with agar powder in volume of preparation, and heat them at 121°C to 122°C. Sterilize at ℃ for 30 minutes and set aside. This medium has the following problems: [0003] 1. High requirements for raw materials: [0004] Potatoes must be fresh, with the skin on. After the potatoe...

AI Technical Summary

Problems solved by technology

Using discolored potatoes as a medium has the following disadvantages: First, the nutrients in the outer layer of potatoes are decomposed to reduce the nutritional content, which affects the growth and metabolism of the cultured bacteria; After boiling, the color becomes light yellowish brown, which affects the color and shape of the fungal flora and the coloring and shape of the conidia

[0005] 2. The preparation steps are too complicated:

[0007] 3. The quality of PDA medium is unstable:

[0013] 4. The growth and metabolic rate of bacteria on PDA medium is unstable:

[0014] Due to the instability of the quality of the PDA medium, the quality of the medium inoculated by the bacteria is different, which will affect the growth and metabolism rate of the bacteria

[0015] 5. The cultivation period is long:

Images