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High-strength photosensitive hydrogel as well as preparation method and application thereof

A high-intensity light, hydrogel technology, used in the introduction of foreign genetic material, tumor/cancer cells, animal cells, etc. using a carrier, can solve problems such as gel damage and weak hydrogel mechanical properties, and achieve high water absorption. , Significant photosensitivity, simple preparation method

Inactive Publication Date: 2014-02-12
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for traditional hydrogels, the dilution effect of water molecules in the gel on the polymer network, the disordered distribution of cross-linking points and the length of chain links, and the inhomogeneity of the cross-linking network during the gelation process cause The mechanical properties of hydrogel are very weak, it is difficult to be used as a soft tissue replacement under high load, and even the touch of tweezers and other utensils during the movement can easily damage the gel, thus limiting its application as a biomaterial

Method used

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  • High-strength photosensitive hydrogel as well as preparation method and application thereof
  • High-strength photosensitive hydrogel as well as preparation method and application thereof
  • High-strength photosensitive hydrogel as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Polymerize monomer oligoethylene glycol methacrylate (6 mg), 2-vinyl-4,6-diamino-1,3,5-triazine (6 mg), SP (0.6 mg) and crosslinker Ethylene glycol diacrylate (8.4 mg) was added to a 1.5 ml centrifuge tube, and after dissolving the monomer and crosslinker with 210 μl of DMSO, the photoinitiator Irgacure 2959 (0.5 mg) was added. The solvent containing monomer, crosslinking agent and initiator is injected into the closed mold, and the mold is irradiated in a UV curing box for 30 minutes to initiate free radical polymerization. Then the mold was opened to take out the gel, rinsed several times with deionized water and soaked for 7 days, and the deionized water was replaced every 12 hours.

[0032] Cell culture and detachment on the gel surface: The obtained gel was sterilized by immersing in medical alcohol for 4 hours, then put into a 96-well plate, and 200 μl of DMEM medium was added to replace the medical alcohol. Add the L929 cell suspension to the culture plate with ...

Embodiment 2

[0035] Polymerize the monomer oligoethylene glycol methacrylate (6 mg), 2-vinyl-4,6-diamino-1,3,5-triazine (12 mg), SP (0.9 mg) and the crosslinker Ethylene glycol diacrylate (12.6mg) was added to a 1.5ml centrifuge tube, and after 315μl of DMSO was used to dissolve the monomer and crosslinker, the photoinitiator Irgacure 2959 (0.7mg) was added. The solvent containing monomer, crosslinking agent and initiator is injected into the closed mold, and the mold is irradiated in a UV curing box for 30 minutes to initiate free radical polymerization. Then the mold was opened to take out the gel, rinsed several times with deionized water and soaked for 7 days, and the deionized water was replaced every 12 hours.

[0036] Cell culture and detachment on the gel surface: The obtained gel was sterilized by immersing in medical alcohol for 4 hours, then put into a 96-well plate, and 200 μl of DMEM medium was added to replace the medical alcohol. Add the L929 cell suspension to the culture ...

Embodiment 3

[0039] Polymerize monomer oligoethylene glycol methacrylate (6 mg), 2-vinyl-4,6-diamino-1,3,5-triazine (18 mg), SP (1.2 mg) and crosslinker Ethylene glycol diacrylate (16.8mg) was added to a 1.5ml centrifuge tube, and after 420μl of DMSO was used to dissolve the monomer and crosslinker, the photoinitiator Irgacure 2959 (0.9mg) was added. The solvent containing monomer, crosslinking agent and initiator is injected into the closed mold, and the mold is irradiated in a UV curing box for 30 minutes to initiate free radical polymerization. Then the mold was opened to take out the gel, rinsed several times with deionized water and soaked for 7 days, and the deionized water was replaced every 12 hours.

[0040] Cell culture and detachment on the gel surface: The obtained gel was sterilized by immersing in medical alcohol for 4 hours, then put into a 96-well plate, and 200 μl of DMEM medium was added to replace the medical alcohol. Add the L929 cell suspension to the culture plate wi...

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Abstract

The invention discloses high-strength photosensitive hydrogel as well as a preparation method and application thereof. The high-strength photosensitive hydrogel is formed by free radical polymerization copolymerization of polyethylene glycol methacrylic ester, 2-vinyl-4,6-diamino-1,3,5-triazine and a double-bond-containing monomer with a spiropyrane structure; the three monomers and an initiator are dissolved in a solvent, unsaturated bonds on the molecules of the monomers are initiated by the initiator, and the hydrogel is prepared through free radical polymerization reaction. The photosensitive hydrogel disclosed by the invention has higher stretching resistance and compression resistance, and has good biocompatibility. Moreover, the hydrogel has the capability of adsorbing DNA (Deoxyribonucleic Acid) transfection cells as well as adjusting attaching and un-attaching behaviors of the cells by illumination.

Description

technical field [0001] The technical field of polymer hydrogel of the present invention, more specifically, relates to the preparation of high-strength hydrogel with the ability to detach cells and transgene effect, that is, polyethylene glycol methacrylate-co-2-vinyl- Preparation and application of 4,6-diamino-1,3,5-triazine-copolymer-copolymer containing spiropyran monomer (PEGMA-co-PVDT-co-PSP) hydrogel. Background technique [0002] Polymer hydrogel is a kind of three-dimensional network polymer with high water content and can maintain the original structure without being dissolved after absorbing water. Because of its characteristics similar to soft tissue and good biocompatibility, it is widely used in biological It has important application value in the field of medical engineering. However, for traditional hydrogels, the dilution effect of water molecules in the gel on the polymer network, the disordered distribution of cross-link points and the length of chain link...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F222/20C08F220/28C08F226/06C08F220/60C08F2/48C08J3/075C12N5/09C12N15/85
Inventor 刘文广王宁张金龙孙亮王朋延
Owner TIANJIN UNIV
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