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Method for producing sericin protein

A technology of sericin and silk gland, which is applied in the field of preparation of sericin, can solve the problem of long cycle of sericin and achieve the effect of shortening the cycle

Inactive Publication Date: 2014-01-29
CHENGDE MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, in the related technology, obtaining sericin protein needs to wait for the mature silkworm to complete the cocooning process, and it will take a certain time for the cooked silkworm to spin the silk to complete the cocooning process, so the cycle of obtaining sericin protein is long

Method used

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  • Method for producing sericin protein
  • Method for producing sericin protein

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Experimental program
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Effect test

Embodiment 1

[0016] Please refer to figure 1 , a preparation method of sericin is provided in an embodiment of the present invention, comprising the following steps:

[0017] Step 101: extracting the middle silk gland of the 5th instar silkworm;

[0018] In step 101, since the middle silk gland contains a large amount of sericin, it is necessary to remove the entire middle silk gland of the fifth instar silkworm for subsequent operations. Specifically, in order to completely remove the middle silk gland, it can be done as follows:

[0019] Press the 5th instar silkworm with the left hand, hold the front chest of the 5th instar silkworm with tweezers with the right hand, gently squeeze the abdomen of the 5th instar silkworm with the left hand, squeeze out the silk gland in the middle, and cooperate with the right hand for traction. Experiments have proved that this method can quickly extract silk glands, and skilled operators can dissect about one silkworm (about 26,000 silkworms) per day...

Embodiment 2

[0032] Step 201: cleaning the silkworm body of the 5th instar silkworm with alcohol;

[0033] In step 201, the silkworm body of the 5th instar silkworm can be disinfected with alcohol to improve the sanitation and safety in the extraction process.

[0034] Step 202: extracting the middle silk gland and silkworm body of the 5th instar silkworm;

[0035] The specific operation method of step 202 is consistent with the method of step 101, and will not be repeated here.

[0036] Step 203: washing, drying and pulverizing the silkworm body in sequence to obtain silkworm powder;

[0037] In step 203, the silkworm bodies obtained in step 202 are washed, dried and pulverized to obtain silkworm meal, which can be used in feed additives.

[0038] Step 204: washing the central silk gland with water;

[0039] Since the surface of the obtained middle silk gland contains a large amount of interstitial fluid, it needs to be washed before homogenization.

[0040] Step 205: adding water to ...

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Abstract

The invention relates to the field of silkworm cultivation processing and in particular relates to a method for producing sericin protein. The method for producing the sericin protein comprises the following steps: picking out middle silk glands of silkworms of five years in age; adding water into the middle silk glands, and homogenizing to obtain homogenate; centrifuging the homogenate to obtain supernate dissolved with sericin protein; drying the supernate, thus obtaining the sericin protein. Compared with a method that silkworm cocoon shells are crushed and then the sericin protein is extracted in the prior art, the method for producing the sericin protein has the advantages that the middle silk glands (massive sericin protein is contained in the middle silk glands) are directly acquired from the silkworms of five years in age while the silkworms do not need complete a cocooning process, and the middle silk glands are homogenized, centrifuged and dried to obtain the sericin protein, so that the production period of sericin protein is shortened.

Description

technical field [0001] The invention relates to the field of silkworm breeding and processing, in particular to a method for preparing sericin. Background technique [0002] Sericin is a globular protein. About 20%-30% of the total weight of silk. When silkworms make cocoons, sericin plays a role of bonding, so that two monofilaments are wrapped together to form cocoon silk. Amino acids with a large number of side chains and hydrophilic groups, such as serine and aspartic acid, have a large market demand because sericin has skin care, beauty and health care functions. [0003] In related technologies, the common method of producing sericin is to grind the cocoon shells containing sericin after the cocoons are cooked, and then use a series of chemical methods to extract sericin from the crushed cocoon shells. . [0004] However, in related technologies, obtaining sericin requires waiting for cooked silkworms to complete the cocooning process, and it takes a certain amount...

Claims

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Application Information

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IPC IPC(8): C07K14/435C07K1/14
CPCC07K14/43586
Inventor 李季生李娜刘沿延王晖贾漫丽夏爱华杨贵明
Owner CHENGDE MEDICAL UNIV
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