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Method for preparing high-color-value gardenia blue pigment

A gardenia blue pigment, high color value technology, applied in chemical instruments and methods, azo dyes, organic dyes, etc., can solve the problems of waste of useful resources, environmental pollution, etc., to improve yield, reduce environmental pollution, Promising effects

Inactive Publication Date: 2014-01-22
NINGDE NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In industry, the waste liquid is usually discarded, which not only wastes useful resources, but also causes environmental pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A method for preparing high color value gardenia blue pigment, which includes the following steps:

[0035] (1) Coarse separation of geniposide from gardenia yellow waste liquid: use ultrafiltration method to roughly separate geniposide, at a pressure difference of 0.03MPa, use a 35K Dalton ultrafiltration membrane to filter, and the number of diafiltration is 4 times , To obtain the geniposide solution after removing the macromolecular impurities;

[0036] (2) Geniposide hydrolysis: using β-glucosidase as the hydrolase, the geniposide solution obtained in step (1) is subjected to enzymatic hydrolysis to obtain a geniposide hydrolyzate, wherein the concentration of the geniposide solution is The hydrolysis temperature is 60°C, the hydrolysis time is 80 min, the enzyme dosage of the β-glucosidase is 360 U / g, the concentration of the geniposide solution is 15 mg / mL, and the pH value of the geniposide solution is 4;

[0037] (3) Recovery of hydrolase: The hydrolyzate of geniposi...

Embodiment 2

[0042] A method for preparing high color value gardenia blue pigment, which includes the following steps:

[0043] (1) Coarse separation of geniposide from gardenia yellow waste liquid: use ultrafiltration method to roughly separate geniposide at a pressure difference of 0.06 MPa, use a 50K Dalton ultrafiltration membrane for filtration, and filter twice. , To obtain the geniposide solution after removing the macromolecular impurities;

[0044] (2) Geniposide hydrolysis: using β-glucosidase as the hydrolase, the geniposide solution obtained in step (1) is subjected to enzymatic hydrolysis to obtain a geniposide hydrolyzate, wherein the concentration of the geniposide solution is The hydrolysis temperature is 55° C., the hydrolysis time is 60 min, the enzyme dosage of the β-glucosidase is 300 U / g, the concentration of the geniposide solution is 12 mg / mL, and the pH value of the geniposide solution is 5;

[0045] (3) Recovery of hydrolase: the hydrolyzate of geniposide obtained after ...

Embodiment 3

[0050] A method for preparing high color value gardenia blue pigment, which includes the following steps:

[0051] (1) Coarse separation of geniposide from gardenia yellow waste liquid: use ultrafiltration method to roughly separate geniposide at a pressure difference of 0.08 MPa and use a 20K Dalton ultrafiltration membrane for filtration. The number of diafiltration is 1 , To obtain the geniposide solution after removing the macromolecular impurities;

[0052] (2) Geniposide hydrolysis: using β-glucosidase as the hydrolase, the geniposide solution obtained in step (1) is subjected to enzymatic hydrolysis to obtain a geniposide hydrolyzate, wherein the concentration of the geniposide solution is The hydrolysis temperature is 45° C., the hydrolysis time is 120 min, the enzyme dosage of the β-glucosidase is 500 U / g, the concentration of the geniposide solution is 5 mg / mL, and the pH value of the geniposide solution is 6;

[0053] (3) Recovery of hydrolytic enzymes: the hydrolyzed gen...

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Abstract

The invention relates to a method for preparing a high-color-value gardenia blue pigment. The invention aims to provide a method for preparing the high-color-value gardenia blue pigment through fully utilizing plenty of gardenoside in gardenia yellow waste liquor. The gardenia blue pigment prepared by adopting the method has the characteristics of high color value, high quality, high purity, environmental friendliness, and the like. The method for preparing the high-color-value gardenia blue pigment comprises the following steps of (1) gardenoside enzyme hydrolysis: carrying out enzyme hydrolysis on the gardenia yellow waste liquor with beta-glucosidase as the hydrolytic enzyme; (2) blue turning of the gardenoside hydrolysate: adding amino acid into the gardenoside hydrolysate obtained in the step (1) to react; (3) ultrafiltration separation of the gardenia blue pigment: carrying out ultrafiltration on the blued solution obtained in the step (2) by using an ultrafiltration membrane; and (4) extraction and purification of the gardenia blue pigment: drying trapped fluid obtained in the step (3) and carrying out extraction through mixing with methyl alcohol or ethyl alcohol to obtain the high-color-value gardenia blue pigment.

Description

Technical field [0001] The invention relates to a method for preparing gardenia blue pigment with high color value. Background technique [0002] Gardenia blue pigment is a water-soluble natural pigment, the aqueous solution is bright and pure blue, the dry powder is blue-black, non-toxic, no odor, easily soluble in water, strong coloring power, stable performance, my country has been in 1989 It was listed in the new varieties of food additives licensed for use in the year, and it is gradually replacing synthetic pigments indigo and brilliant blue, and is used in food, medicine and cosmetics industries. The preparation of gardenia blue pigment mostly adopts the conversion of gardenoside by microbial fermentation or β-glucosidase hydrolysis. The separation and purification methods of gardenia blue pigment mainly include macroporous adsorption resin method and membrane separation method. If only the macroporous adsorption resin method and membrane separation method are used, the o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/16C09B61/00
Inventor 陈峰陈剑锋
Owner NINGDE NORMAL UNIV
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