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Swine flu H1N1 and H3N2 subtype bivalent inactivated vaccine

A bivalent inactivated vaccine, H3N2 technology, applied in microorganism-based methods, biochemical equipment and methods, medical preparations containing active ingredients, etc. Effectiveness against swine flu, good safety, good safety

Active Publication Date: 2013-12-25
HUAZHONG AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] So far, there have been no reports of bivalent inactivated vaccines for swine influenza H1N1 and H3N2 subtypes

Method used

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  • Swine flu H1N1 and H3N2 subtype bivalent inactivated vaccine
  • Swine flu H1N1 and H3N2 subtype bivalent inactivated vaccine
  • Swine flu H1N1 and H3N2 subtype bivalent inactivated vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Preparation Example

[0028] 1. Isolation and identification of related strains of swine influenza virus

[0029] 1.1 Isolation and identification of swine influenza virus H1N1TJ strain

[0030] Nasal swabs were collected from pigs suspected of influenza infection in a pig farm, chicken embryos were inoculated, and the allantoic fluid of chicken embryos was collected. After detection by RT-PCR, it was determined to be the subtype of swine influenza virus H1N1, and the virus strain was named pig Influenza virus H1N1SIV TJ strain. The specific operation steps are as follows:

[0031] 1.1.1 Chicken embryo multiplication

[0032] Add double antibody (final concentration: 10000U / ml) to the disease material treatment solution, incubate at 33-35°C for 30 minutes, inoculate 9-11-day-old SPF chicken embryos through the chorioallantoic cavity, and inoculate 4 chicken embryos per disease material 0.2ml per embryo. The inoculated chicken embryos were incubated at 33-...

Embodiment 2

[0239] The safety test of embodiment 2 swine influenza virus bivalent inactivated vaccine of the present invention

[0240] (1) Vaccines and experimental animals

[0241] Three batches of swine influenza virus bivalent inactivated vaccines trial-produced by the inventors were used, and the batch numbers were 110301, 110302, and 110403 respectively. The pig breed used for the trial was Da Ke, which was provided by Xianning Shentong Animal Husbandry Co., Ltd., Hubei Province, and was a commercial pig breed.

[0242] This embodiment has tested the safety of the above 3 batches of laboratory vaccines, including the safety of a single-dose vaccination of piglets and pregnant sows, the safety of repeated single-dose vaccinations and an overdose vaccination of the bivalent inactivated influenza virus vaccine vaccine. security.

[0243] (2) The safety of a single-dose vaccination for weaned piglets

[0244] Three batches of influenza virus bivalent inactivated vaccine vaccines were...

Embodiment 3H1

[0277] The vaccine effectiveness test of the bivalent inactivated vaccine of embodiment 3 H1N1 subtype swine influenza virus and H3N2 subtype swine influenza virus

[0278] (1) Bivalent inactivated vaccines of swine influenza H1N1 and H3N2 subtypes and experimental animals

[0279] Three batches of bivalent inactivated vaccines of swine influenza H1N1 and H3N2 subtypes trial-produced by the inventors were used, and the batch numbers were 110301, 110302, and 110403 respectively. The hemagglutination inhibitory antibodies of swine influenza virus H1N1 and H3N2 subtypes at 25 to 30 days old were negative ( HI≤1:8) Healthy piglets were used as experimental animals, provided by Xianning Shentong Animal Husbandry Co., Ltd., Hubei Province.

[0280] (2) Vaccination

[0281] Each batch of vaccines was used to immunize 10 pigs, and each pig was injected with 2ml of the vaccine through the neck muscle. At the same time, 10 unimmunized pigs with the same conditions were set as controls....

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Abstract

The invention belongs to the technical field of animal virology and in particular relates to a swine flu H1N1 and H3N2 subtype bivalent inactivated vaccine and an application thereof. The bivalent inactivated vaccine is prepared from H1N1 subtype swine flu viruses, namely H1N1 SIV TJ strains with a preservation number of CCTCC (China Center For Type Culture Collection) NO:V201107 and H3N2 subtype swine flu viruses, namely HuN-1 strains with a preservation number of CCTCC NO:V201308. The swine flu H1N1 and H3N2 subtype bivalent inactivated vaccine is good in safety and has an immunity protection effect reaching more than 80%.

Description

technical field [0001] The invention belongs to the technical field of animal virology, and in particular relates to a bivalent inactivated vaccine of swine influenza H1N1 and H3N2 subtypes. The bivalent inactivated vaccine is jointly prepared by H1N1TJ strain + H3N2HuN-1 strain. The invention also relates to the application of the inactivated vaccine prepared from the H1N1 subtype and H3N2 subtype susceptible virus strains. Background technique [0002] Swine influenza virus (swine influenza virus, SIV) belongs to Orthomyxoviridae type A influenza virus genus, type A influenza is isolated from many species including humans, pigs, horses, mink, marine animals, domestic and wild birds (Qi , Pang et al. 2009). Surface antigens HA and NA are very important for influenza virus host range, antigenicity, pathogenicity, detection and diagnosis. It can be divided into different subtypes according to its surface antigens hemagglutinin (HA) and neuraminic acid (NA) (Vincent, Swenson...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00A61K39/295A61K39/145A61P31/16C12R1/93
Inventor 金梅林杨影徐高原
Owner HUAZHONG AGRI UNIV
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