Construction method of tissue engineering epidermis model
A tissue engineering and construction method technology, applied in medical science, prosthesis, etc., can solve problems such as detection of fluid leakage, and achieve the effects of good mechanical properties, good adhesion, and guaranteed mechanical strength
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Embodiment 1
[0029] Polypropylene melt-blown non-woven fabric, hot-pressed at 140°C into a disc shape.
[0030] Commercially available mulberry silk, immersed in 60°C Na 2 CO 3 degumming in aqueous solution, Na 2 CO 3 The concentration of the aqueous solution is 0.2%, 30 minutes each time, 3 times in total, and dried at 70°C after washing with water. Dissolve in a ternary solution of CaCl2, ethanol, and water at 60°C (molar ratio 1:2:8), the dissolution time is 2 hours, put it into a dialysis bag, and dialyze under running water. A silk fibroin solution with a concentration of 3% was obtained. Soak the disc-shaped non-woven fabric in the silk fibroin solution, take it out, dry it in vacuum, and post-treat it with absolute ethanol, with 10 4 / ml Hacat cells were inoculated, cultured on the air-liquid surface, and the tissue engineering epidermal model was constructed. figure 2 is the electron micrograph of the blank scaffold without seeding cells, image 3 It is an electron microgra...
Embodiment 2
[0032] Polyethylene and polypropylene two-component melt-blown non-woven fabric, hot-pressed at 130°C into a disc shape.
[0033] Commercially available mulberry silk is immersed in a neutral soap solution at 90-100°C for degumming, washed with water and dried at 70°C. Dissolved in CaCl at 95°C 2In the aqueous solution, the dissolution time is 10 minutes, put into a dialysis bag, and dialyze under running water washing. A silk fibroin solution with a concentration of 6% was obtained. Soak the disc-shaped non-woven fabric in the silk fibroin solution, take it out, dry it in vacuum, and post-treat it with absolute ethanol, with 10 4 / ml Hacat cells were inoculated, cultured on the air-liquid surface, and the tissue engineering epidermal model was constructed.
Embodiment 3
[0035] Polyamide spun-bonded non-woven fabric, heat-pressed at 200°C into disc shape.
[0036] Commercially available mulberry silk was degummed by immersing in deionized water under high temperature and pressure, washed with water and dried at 70°C. Dissolved in CaCl at 70°C 2 , ethanol, and water in a ternary solution (molar ratio 1:2:8), the dissolution time is 4 hours, put into a dialysis bag, and dialyze under running water. A silk fibroin solution with a concentration of 9% was obtained. Soak the disc-shaped non-woven fabric in the silk fibroin solution, take it out, dry it in vacuum, and post-treat it with absolute ethanol, with 10 5 / ml Hacat cells were inoculated, cultured on the air-liquid surface, and the tissue engineering epidermal model was constructed.
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