Micro-fluidic chip detection system based on single-cell multi-parameter representation

Abstract

The invention discloses a micro-fluidic chip detection system based on single-cell multi-parameter representation. The micro-fluidic chip detection system mainly comprises a micro-fluidic chip, an optical detection module, an impedance detection module and a processor, wherein the micro-fluidic chip is formed by sequentially aligning and bonding three layers of substrates, two symmetrical transport focusing runners and two symmetric outlet runners are arranged on the runner layer, a cross-shaped structure is formed by a junction of the two transport focusing runners and the outlet runners, electrodes of the upper layer of substrate and the lower layer of substrate are oppositely arranged to form a counter electrode structure, and each of the two outlet runners is provided with the counter electrode structure. According to the micro-fluidic chip detection system disclosed by the invention, a plurality of parameter of a single cell can be simultaneously represented, the accuracy and the sensitivity of cell detection are improved, a sheath liquid is not needed, and complex immunolabelling pretreatment is not needed. The micro-fluidic chip detection system has the advantages of low cost, simplicity in operation, high flux, high integration and automation degree and the like. The micro-fluidic chip detection system disclosed by the invention can be widely applied to the fields of cell cycle, cell differentiation, drug screening, early diagnosis and treatment of illnesses, and the like.

Description

technical field [0001] The invention relates to the field of manipulation and detection of microfluidic chips and micro-nano biological particles, in particular to a microfluidic chip detection system based on focusing of viscoelastic particles and simultaneously characterizing the mechanical and electrical properties of single cells. Background technique [0002] Cells are the basic unit of life activities, and their unique biochemical and biophysical properties enable them to achieve specific functions and adapt to the surrounding environment. Biophysical properties play an important role in various biological processes of cells, and participate in the regulation of gene expression, differentiation, migration and metabolism of cells, and the physiological changes in cells are accompanied by physical modification and recombination. It can be seen that physical properties are effective markers for characterizing cells. The analysis of biophysical properties of single cells c...

AI Technical Summary

Problems solved by technology

These microfluidic detection chips have their own advantages in the characterization of single-cell biophysical properties, but they cannot have high detection throughput and detection accuracy at the same time.

More importantly, the existing microfluidic chips can only analyze a single performance parameter of cells, and cells are extremely diverse, and a single characterization parameter cannot effectively distinguish cells, which is likely to cause false positives, false negatives, etc.

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