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Preparation technology and medical applications of compound specific transfer factor

A technology of transfer factor and preparation process, which is applied in the field of biomedicine, can solve the problems of narrow anti-infectious disease spectrum, unstable activity, and low yield, and achieve the effects of broad anti-infectious disease spectrum, stable product quality, and high yield

Active Publication Date: 2013-11-20
苟鸿鹰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The invention discloses a preparation process of compound specific transfer factor, which solves the shortcomings of traditional preparation of transfer factor, such as unstable activity, low yield, small scale and narrow anti-infective disease spectrum

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1) After absorbing the nutrient solution, replace the donkey leukocyte strain with 1-2% seed solution with fresh culture solution, continue to cultivate at 37 degrees, and observe 5-7 days after inoculation, and about 80% of the cells have lesions , when there is a significant difference from the control cells, the virus content per milliliter is ≥105TCD50, and the same amount is respectively introduced into AIDS virus culture stock solution and BCG, and inactivated by hydrostatic pressure (300Mpa, 30min);

[0027] 2) Add 0.6% deketone nitrogen 101 to the total amount of virion stock solution, mix on a shaker for 1.5 hours, use a nano-collider to process under the conditions of 200-300 pressure and 20 nanometers, use gel filtration to remove ketone nitrogen 101, add 30 % fat emulsion is used as an immune adjuvant to obtain the lysed vaccine antigen;

[0028] 3) immunize 3 healthy horses and donkeys each, 100 laying hens, and 5 dairy cows (dairy goats) with the antigens ...

Embodiment 2

[0034] The pentavalent vaccine for dogs includes five attenuated viruses including rabies, canine febrile fever, canine parainfluenza, canine adenovirus and canine parvovirus, which are kept, identified and supplied by the China Veterinary Drug Control Institute. The five kinds of viruses used include three grades of original virus seeds, basic virus seeds and production virus seeds, and the third-level virus seeds are all cell cultures adapted to the virus seeds, of which the 1-5 generation is the original virus seed; the 6-10 generation is the Basic poisonous species; 11-15 generation poisonous species for production. All three types of viruses are acceptable, and the original virus seed cell culture stock solution is the best.

[0035] Vaccines are mixed in batches of 10,000 doses, that is, each dose contains 15 minimum immune doses of rabies, canine distemper and canine parainfluenza vaccines, and 10 minimum immune doses of canine parvovirus and canine adenovirus The dosa...

Embodiment 3

[0043] 1) Mix blue bacteria net and type b influenza "Haemophilus conjugate vaccine" in equal proportions, then add 30% fat emulsion to mix according to the ratio of the above total amount of 1:1, and sterilize with hydrostatic pressure 200Mpa for 20 minutes live;

[0044] 2) Add 0.5% deketone nitrogen 101 to the total amount of virion stock solution, mix on a shaker for 1 hour, use a nano-collider to process under the conditions of 250 pressure and 30 nanometers, remove ketone nitrogen 101 by gel filtration, and add 20% fat Milk is used as an immune adjuvant to obtain the specific transfer factor antigen of the lysed compound combined with bacteria;

[0045] 3) Select 4 horses and 10 pigs for the above-mentioned antigen immunization injection, collect the animal immune system organ tissue, crush the tissue through a meat grinder, add 2 times normal saline, make a homogenate with a homogenizer, filter with a filter pump, and use the filtrate Nano-collider treatment, adjust th...

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PUM

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Abstract

The invention discloses a preparation technology and medical applications of a compound specific transfer factor. By adopting nanotechnology and chemical technology method, pathogenic virus, bacteria, parasites, and various tumors are prepared into split vaccines or subunit vaccines, which can prevent infectious diseases of human and livestock, according to the requirements on biological products. Then the vaccines are prepared into specific immunity antigens for animals by taking the fat emulsion as the auxiliary materials. Various animals are injected with the immunity antigens, the antibody levels, which are generated by the corresponding antigens, of the immunity animals are measured during the period. After the antibody levels meet the requirements, the animals qualified are immunized. The preparation technology comprises following steps: grinding the immunized organ tissues with a meat grinder, adding normal saline, filtering with a filtration pump, then processing the materials with a nano technology, and finally subjecting the materials to an ultra-filtration treatment so as to obtain the compound specific transfer factor. The specific transfer factor has the advantages of stable activity, high yield and large scale, the anti-septic disease spectrum is wide, the technology method is advanced, and the product quality is stable. The resources are fully utilized, and the production cost is largely reduced. Different user needs can be fully satisfied; the production period of the product is short, thus the work efficiency is largely improved; the drug group can be prepared into various dosage forms, and the product is user-friendly.

Description

[0001] technical field [0002] The invention discloses a preparation process of a composite specific transfer factor, and also provides a medical application of the composite specific transfer factor, belonging to the technical field of biomedicine. Background technique [0003] The immune system is an important immune system in humans and animals. It refers to the general term for a series of organs, cells and molecules that participate in the immune response against pathogens and perform immune functions in humans and animals. The system is composed of immune organs, immune cells or immune molecules, and is the material basis for immune response. The immune system is composed of central immune organs and peripheral immune organs. Central includes: bone marrow, thymus and bursa of Fabricius; peripheral includes: lymph nodes, spleen, Hader's glands, mucosal immune system (intestinal mucosa, mammary duct mucosa, uterine mucosa, etc.). It can be said that the immune system ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/12A61K35/26A61K35/28A61K35/14A61K39/395A61P37/04A61P31/18A61P31/22A61P31/16A61P31/20A61P31/04A61P33/02A61P35/00A61P31/14A61K35/15
Inventor 苟鸿鹰苟仕金
Owner 苟鸿鹰
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