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Methods for preparing and preserving dictyophora rubrovolvata culture

A technology for preparing Bamboo fungus and bacteria strains, which can be applied in the directions of botanical equipment and methods, horticulture, and application, and can solve problems such as strain degradation and yield reduction in cultivation areas.

Inactive Publication Date: 2013-11-20
GUIZHOU INST OF BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Dictyophora rubrovolota Zang, Ji et Lio is a rare and delicious edible fungus. However, during the long cultivation process in Guizhou, the species of Dictyophora rubrovolota Zang, Ji et Lio has been severely degraded, resulting in thousands of acres of cultivated area reduced to zero

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method for preparing and preserving the strain of Dictyophora edulis, specifically:

[0029] 1. Culture medium preparation Wash and peel the potatoes, cut 180g-220g into thin slices and put them in a container, add 450ml-550ml of water, boil over low heat until the slices are crisp and not rotten, filter with four layers of wet gauze, and take the filtrate Standby; put 80g-120g of pine needles into a container, add 450-550ml of water and boil for 10-20 minutes, filter with four layers of wet gauze, take the filtrate for later use, combine the obtained filtrates, make up to 950-1050ml, and add to the Add 18g-22g of agar powder, 18-22g of maltose, 4g-6g of peptone to the final solution, boil, adjust the pH to pH7.5-8.0 with plant ash aqueous solution; Make a slant medium into a slant for subsequent use;

[0030] 2. Strain isolation and inoculation culture Select mature unopened bamboo fungus eggs, remove the sediment, and carry out sterilization treatment; pick a small...

Embodiment 2

[0033] A preparation method of Dictyophora edulis strain, specifically:

[0034] 1. Culture medium preparation Wash the potatoes, peel them, take 180g and cut them into thin slices, put them in a container, add 450ml of water, boil over low heat until the slices are crispy but not rotten, filter them with four layers of wet gauze, and take the filtrate for later use; take the pine needles Put 120g into the container, add 550ml of water and boil for 15 minutes, filter with four layers of wet gauze, take the filtrate for later use, combine the obtained filtrates, make up to 1000ml, add 18g of agar powder, 22g of maltose to the solution after making up, 4g of peptone, boiled, adjust the pH to pH7.5-8.0 with a plant ash aqueous solution; put it into test tubes while hot, carry out high-pressure steam sterilization, take them out and place them on a slope for later use;

[0035] 2. Strain isolation and inoculation culture Select mature unopened bamboo fungus eggs, remove the sedime...

Embodiment 3

[0038] A method for preparing and preserving the strain of Dictyophora edulis, specifically:

[0039] 1. Culture medium preparation Wash and peel the potatoes, take 220g and cut them into thin slices, put them in a container, add 550ml of water, boil over low heat until the slices are crisp and not rotten, filter with four layers of wet gauze, and take the filtrate for later use; take pine needles Put 80g into a container, add 450ml of water and boil for 10-20 minutes, filter with four layers of wet gauze, take the filtrate for later use, combine the obtained filtrates, make up to 1000ml, add 22g of agar powder, maltose 18g, 6g of peptone, boiled, adjust the pH to pH7.5-8.0 with plant ash aqueous solution; put it into test tubes while hot, sterilize them with high-pressure steam, take them out and place them on a slope for later use;

[0040] 2. Strain isolation and inoculation culture Select mature unopened bamboo fungus eggs, remove the sediment, and carry out sterilization ...

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Abstract

The invention discloses methods for preparing and preserving dictyophora rubrovolvata culture. The method for preparing the dictyophora rubrovolvata culture includes boiling potatoes by warm fire until slices are crisp but not tender, filtering the slices by four layers of wet gauze and obtaining filter liquor; boiling pine needles to acquire filter liquor; combining the two types of acquired filter liquor, adding agar powder, maltose and peptone into combined filter liquor to obtain a mixture, boiling the mixture and regulating the mixture by plant ash aqueous solution until the potential of hydrogen of the mixture ranges from pH7.5 to pH8.0; separately filling the mixture into test tubes, then sterilizing the test tubes and placing the test tubes on an oblique plane; selecting a small tissue block on each dictyophora stem and indusium part, transferring the small tissue blocks on media on the oblique plane, cultivating the small tissue blocks for 5 to 7 days at the temperature of 24 DEG C, and transferring hyphae into other tubes at one step to enlarge cultivation after the hyphae fully grow in the test tubes on the oblique plane so as to obtain the dictyophora rubrovolvata stock culture. The method for preserving the dictyophora rubrovolvata culture includes mixing tree branches, bran, soybean meal, maltose, calcium superphosphate and sawdust to obtain a mixture, regulating the mixture until the pH (potential of hydrogen) of the mixture reaches 8, filling the mixture into culture bottles and sterilizing the culture bottles; inoculating the dictyophora rubrovolvata culture into compost at the temperature of 30 DEG C under a sterile condition; cultivating the dictyophora rubrovolvata culture at the temperature of 24 DEG C, preserving hyphae at the temperature of 10 DEG C for 30 days after the hyphae fully grow in the compost and then transferring the hyphae to preserve the hyphae at the temperature of 4 DEG C.

Description

technical field [0001] The invention relates to the field of strain preparation and preservation of edible fungi, in particular to a method for preparation and preservation of strains of Dictyophora edulis. Background technique [0002] Dictyophora rubrovolota Zang, Ji et Lio is a rare and delicious edible fungus. However, during the long cultivation process in Guizhou, the species of Dictyophora rubrovolota Zang, Ji et Lio has been severely degraded, resulting in thousands of acres of cultivated area reduced to zero . Therefore, the artificial cultivation of Dictyophora spp. and the cultivation of excellent Dictyophora spp. strains are the key to successful cultivation. Therefore, the preparation and preservation of excellent Dictyophora spp. strains will play an important role. Contents of the invention [0003] In order to solve the above-mentioned technical problems, the object of the present invention is to provide a method for preparing and preserving Dictyophora ed...

Claims

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Application Information

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IPC IPC(8): A01G1/04
Inventor 邹方伦潘高潮何云松龙汉武刘耀
Owner GUIZHOU INST OF BIOLOGY
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