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Application of Rhodoccus phridinivorans to degradation of zearalenone

A technology of zearalenone and rhodococcus pyridine, applied in the field of microorganisms, can solve problems such as unstable effects, large loss of nutrients, and secondary pollution

Inactive Publication Date: 2013-09-25
ACAD OF STATE GRAIN ADMINISTRATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the common ZEN physical or chemical detoxification methods can reduce the toxicity or content of ZEN in various grains, the effect of these methods is unstable, the loss of nutrients is large, and the components of the degradation products are complex, with large toxic and side effects, which are easy to cause secondary Pollution

Method used

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  • Application of Rhodoccus phridinivorans to degradation of zearalenone
  • Application of Rhodoccus phridinivorans to degradation of zearalenone
  • Application of Rhodoccus phridinivorans to degradation of zearalenone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Cultivation of Rhodococcus pyridine (CGMCCNo4.1825)

[0022] Training conditions:

[0023] (1) Incline culture: culture time 2-3 days, medium composition (1 liter): 10g tryptone, 5g yeast extract, 10g NaCL, 15g agar (solid), the optimum growth temperature is 26-28℃.

[0024] (2) Seed culture: Pick activated single colony from the slope in the ultra-clean bench and put it into the seed culture solution, shake the bacteria at 28° C. for 2-3 days, and rotate at 220 rpm. Seed culture solution composition (1 liter): 10g tryptone, 5g yeast extract, 10g NaCl.

[0025] (3) Fermentation: Inoculate the seed culture medium according to the inoculum amount of 5%, shake and cultivate for 10-14 days, and the rotating speed is 220rpm. The composition of the culture medium is the same as above.

Embodiment 2

[0026] Example 2 Degradation of zearalenone by Rhodococcus pyridine (CGMCCNo4.1825)

[0027] For the co-culture experiment of Rhodococcus pyridinevorum (CGMCCNo4.1825) and ZEN, the pretreatment and detection conditions of zearalenone HPLC detection refer to the national standard GB / T 5009.209-2008, and a certain amount of fermentation broth is taken every day with high-efficiency The content of ZEN was determined by liquid chromatography.

[0028] Cultivate experimental steps (1), (2) with embodiment 1,

[0029] (3) Zearalenone was added to the culture medium, the concentration of zearalenone was 100 μg / mL, other conditions were the same as in Example 1, and the fermentation broth without ZEN was used as a control.

[0030] Table 1 Degradation effect of Rhodococcus pyridine on ZEN

[0031]

[0032]

[0033] Conclusion: The co-culture experiment of Rhodococcus pyridinevorum (CGMCCNo4.1825) and ZEN has a good effect on the degradation of 100 μg / mL ZEN. The degradation e...

Embodiment 3

[0034] Example 3 Degradation of zearalenone by metabolites of Rhodococcus pyridinevorum (CGMCCNo4.1825)

[0035] Centrifuge the fermented broth of Rhodococcus pyridineivorum (CGMCCNo4.1825) prepared in Example 1 at 4000r / min for 10min, filter and sterilize the supernatant fermented broth with a 0.2μm sterile microporous membrane, then add ZEN at a concentration of 20μg / mL , after the supernatant fermentation broth passed through the 0.22 μm pore size aqueous membrane was treated in a constant temperature water bath at 100°C for 10 minutes, ZEN was added at a concentration of 20 μg / mL as a control. The pretreatment and detection conditions of zearalenone HPLC detection refer to the national standard GB / T 5009.209-2008, and a certain amount of supernatant fermentation broth is taken every day to determine the content of ZEN by HPLC.

[0036] Table 2 Degradation effect of metabolites of Rhodococcus pyridine on ZEN

[0037]

[0038] Conclusion: The metabolites of Rhodococcus p...

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Abstract

The invention discloses an application of Rhodoccus phridinivorans to degradation of zearalenone and belongs to the field of microorganisms. The Rhodoccus phridinivorans is characterized in that the preserve number is CGMCC (China General Microbiological Culture Collection) NO4.1825. The application has the advantages that the Rhodoccus phridinivorans is gram-positive bacterium which can be used for producing metabolite having obvious degradation effect on zearalenone in a microbial fermentation manner and has a good prospect in development of fungaltoxin biological detoxication agents.

Description

technical field [0001] The invention relates to the application of a strain of Rhodococcus pyridinevorum in degrading zearalenone, which belongs to the field of microorganisms. Background technique [0002] Zearalenone (ZEN) is one of the most widespread fusarium toxins in the world. ZEN has estrogen-like effects, and mainly pollutes corn, wheat, rice, barley, millet, oats and other grains, and can directly enter human and animal bodies through contaminated grains and other crops, causing acute and chronic poisoning of animals, causing abnormal reproductive function and even Death can cause huge economic losses to livestock farms, and can also accumulate after entering the human body through contaminated meat, milk and other animal foods, causing harm to the human body. ZEN has strong reproductive toxicity and teratogenic effect, and can cause estrogen toxicity in animals and humans, so it has been paid more and more attention. Existing studies have shown that mononuclear ...

Claims

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Application Information

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IPC IPC(8): A23L1/105A23L1/10A23L7/104A23L7/10
Inventor 伍松陵孙长坡常晓娇沈晗任保中吴子丹
Owner ACAD OF STATE GRAIN ADMINISTRATION
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