Detection method for CVA16 in environmental water body
A detection method and virus technology, applied in the directions of microorganism-based methods, microbial determination/inspection, biochemical equipment and methods, etc., which can solve the problems of complex factors affecting detection, inability to accurately qualitatively or quantitatively detect CVA16, and no CVA16 detection method, etc. question
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[0074] The equipment and reagents used in this example are as follows:
[0075] (1) High-speed refrigerated centrifuge, the maximum speed is 14000r / min;
[0076] (2) 4°C, -80°C refrigerator
[0077] (3) Centrifuge tubes, 50mL, 1.5mL, 0.5mL, 0.2mL;
[0078] (4) Micro UV spectrophotometer;
[0079] (5) Constant temperature metal heater
[0080] (6) Bacterial shaker, bacterial incubator
[0081] (7) Real-time fluorescence quantitative PCR instrument;
[0082] (8) Gradient qualitative PCR instrument;
[0083] (9) Electrophoresis apparatus;
[0084] (10) Gel imager
[0085] (11) pMD19-T vector;
[0086] (12) Competent Escherichia coli DH5α:
[0087] (13) Hydrochloric acid, NaCl solution, MgCl 2 solution, etc.;
[0088] (14) Polyethylene glycol (PEG-6000) with a molecular weight of 6000, glucose, etc.;
[0089] (15) 5mol / L NaCl solution;
[0090] (16) IPTG / X-gal / Amp plate;
[0091] (17) LB liquid medium;
[0092] (20) Total RNA extraction kit;
[0093] (18) Reverse t...
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