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Lyophilized vaccine for porcine rotavirus (PRV)

A porcine rotavirus and vaccine technology, which is applied in the directions of antiviral agents, viral antigen components, and medical preparations with non-active components, etc., to achieve the effects of low cost of raw materials, simple operation, and extended shelf life

Active Publication Date: 2013-09-18
YEBIO BIOENG OF QINGDAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there is no porcine rotavirus freeze-dried vaccine that can effectively store for a long time

Method used

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  • Lyophilized vaccine for porcine rotavirus (PRV)
  • Lyophilized vaccine for porcine rotavirus (PRV)
  • Lyophilized vaccine for porcine rotavirus (PRV)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] In the field of vaccine lyoprotectant preparation, the selection and ratio of the protective agent components have an important impact on the preservation effect of the vaccine. In order to enable the swine fever vaccine of the present invention to be preserved for a long time, the applicant has carried out long-term research on the components of the lyoprotectant of the present invention, and optimized the proportioning concentrations of each other, so that each component has the greatest complex matching effect.

[0024] 1. Preparation of lyoprotectant: divided into two parts, liquid A and liquid B.

[0025] 1) Preparation of liquid A: Weigh 48g of NZ-amine, 6g of monopotassium glutamic acid, 400g of sucrose, and 30g of hydrolyzed milk protein; dissolve the above ingredients in 800ml of water for injection in order, shake well after fully dissolving, and add water for injection After reaching 1000ml, shake well, filter and sterilize with a 0.22μm filter membrane, and...

Embodiment 2

[0035] 1. Preparation of lyoprotectant:

[0036] Preparation of lyoprotectant: divided into two parts, liquid A and liquid B.

[0037] 1) Preparation of liquid A: Weigh 80g of NZ-amine, 10g of monopotassium glutamic acid, 500g of sucrose, and 50g of hydrolyzed milk protein; dissolve the above ingredients in 800ml of water for injection in order, shake well after fully dissolving, and add water for injection to 1000ml, sterilized by filtration with a 0.22μm filter membrane, and stored in a 37°C greenhouse for future use.

[0038] 2) Preparation of liquid B: Take 120g of gelatin, fully dissolve it with 800ml of water for injection at 70-80°C, add water for injection to 1000ml, autoclave at 121°C and 15 pounds for 30 minutes, and store at 37°C after sterilization.

[0039] 3) Proportion according to the volume ratio of liquid A: liquid B = 1:1, and mix well to get the heat-resistant freeze-drying protective agent.

[0040] 2. Vaccine preparation

[0041] 1. After porcine rotav...

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Abstract

The invention relates to a lyophilized vaccine for porcine rotavirus (PRV). The lyophilized vaccine consists of the PRV serving as an antigen, and a protective agent, wherein the protective agent is prepared from the following substances in percentage by mass: 2.4-4% of NZ-amine, 0.3-0.5% of mono-potassium glutamate, 16-20% of sucrose, 6-20% of lactoalbumin hydrolysate, 1-1.6% of gelatin and the balance of water. According to the vaccine disclosed by the invention, the used lyophilized protective agent is made from raw materials which are low in cost, and the operation is simple, so that the vaccine can be produced in a large scale. After the vaccine prepared from the protective agent is stored for 24 months at the temperature of 2-8 DEG C, the potency of the vaccine is not smaller than 10<5.7>TCID50 / head (Chinese Standard), so that the storage life of the vaccine is prolonged effectively.

Description

technical field [0001] The invention belongs to the technical field of porcine vaccine preparation, and in particular relates to a porcine rotavirus freeze-dried vaccine. Background technique [0002] Porcine rotavirus (PRV) is an infectious disease of pigs caused by rotavirus characterized by diarrhea. It mainly causes severe gastroenteritis and villous atrophy in pigs that have not fed colostrum. The main clinical symptoms are nausea, vomiting, and diarrhea, and adult pigs generally show recessive infection. Given that porcine rotavirus infection is common and harmful, it is of great significance to vaccinate pigs to prevent porcine rotavirus infection. [0003] At present, the attenuated live vaccine is one of the main vaccines to prevent infectious diseases of livestock and poultry. In my country's animal vaccines, live vaccines account for a considerable proportion. However, the freeze-dried protective agents commonly used by domestic vaccine companies have simple for...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/42A61K47/26A61K47/18A61K39/15A61P1/12A61P31/14A61K31/43A61K31/7036
Inventor 孙健胡潇邹桂荣李芳王龙张维
Owner YEBIO BIOENG OF QINGDAO
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