Preparation method for egg yolk antibody inactivated vaccine resisting loach pathogenic vibrio cholerae

Abstract

The invention discloses a preparation method for an egg yolk antibody inactivated vaccine resisting loach pathogenic vibrio cholerae. The preparation method comprises the following steps of: inactivating and centrifuging a loach pathogenic vibrio cholerae NQ1 strain to obtain an inactivated thallus; then preparing astragalus membranaceus leachate; then preparing an oil phase from white oil, aluminium stearate and Span-80, and mixing the inactivated thallus with the astragalus membranaceus leachate to prepare the water phase of the vaccine; and finally mixing, emulsifying and grinding the oil phase and the water phase for 5 minutes in a volume ratio of 2: 1 to prepare the loach pathogenic vibrio cholerae NQ1 oil emulsion inactivated vaccine taking astragalus membranaceus and white oil as a combined adjuvant. The loach pathogenic vibrio cholerae oil emulsion inactivated vaccine disclosed by the invention can be used for immunizing adult laying hens, and then obtaining the high-immunity egg yolk antibody resisting loach pathogenic vibrio cholerae, as well as is capable of enhancing the immunity of loaches for pathogenic vibrio cholerae, preventing and treating the diseases caused by pathogenic vibrio cholerae on loaches, increasing the yield of loach culture, and improving the economic benefits of loach culture and production; and the problem that the bacteriosis due to pathogenic vibrio cholerae is easy to cause during the existing loach culture and production process is solved.

Description

technical field [0001] The invention relates to a preparation method of an inactivated vaccine, in particular to a preparation method of an egg yolk antibody inactivated vaccine against loach pathogen Vibrio cholerae. Background technique [0002] loach( Misgurnus anguillicaudatus L) is one of the main freshwater economic fishes in my country. Its meat is fresh and tender, rich in nutrition, and has dual values ​​of food and medicine, and is favored by consumers. In recent years, due to the large demand for Chinese loach from surrounding markets such as South Korea and Japan, the artificial intensive farming of loach has developed rapidly, especially in the northern Jiangsu area. The development of loach farming industry. In recent years, in the investigation of loach bacterial diseases, we found that Vibrio cholerae is one of the main epidemic pathogens of loach bacterial diseases, which seriously endangers loach aquaculture production. The bacterium is widely distribut...

AI Technical Summary

Problems solved by technology

[0003] In the prevention and control of loach bacterial diseases, chemically synthesized drugs or antibiotics are often used to control the occurrence and prevalence of diseases. Environmental pollution, and lead to a large number of drug-resistant microorganisms, and has become a barrier to export earnings, seriously affecting the development of loach farming

At present, it is difficult to control the occurrence and prevalence of diseases caused by Vibrio cholerae in the production of loach farming, and there is a lack of an easy-to-use immune preparation against Vibrio cholerae, the pathogen of loach in the market.