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Lyophilized vaccine for classical swine fever

A vaccine and freeze-drying technology, which can be applied to medical preparations with inactive ingredients, inactive ingredients of polymer compounds, and medical components of antibodies. The effect of time limit, low cost of raw materials and simple operation

Inactive Publication Date: 2013-09-18
YEBIO BIOENG OF QINGDAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the storage temperature of live swine fever vaccines in my country is still -15°C. Imported vaccines have caused a huge market impact on domestic vaccines, affecting the development of domestic biological products companies.

Method used

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  • Lyophilized vaccine for classical swine fever
  • Lyophilized vaccine for classical swine fever
  • Lyophilized vaccine for classical swine fever

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. Preparation of lyoprotectant: divided into two parts, liquid A and liquid B.

[0026] 1) Preparation of liquid A: Weigh 48g of NZ-amine, 6g of monopotassium glutamic acid, 400g of sucrose, and 30g of hydrolyzed milk protein; dissolve the above ingredients in 800ml of water for injection in order, shake well after fully dissolving, and add water for injection After reaching 1000ml, shake well, filter and sterilize with a 0.22μm filter membrane, and store in a 37°C greenhouse for future use.

[0027] 2) Preparation of liquid B: Take 80g of gelatin, fully dissolve it with 800ml of water for injection at 70-80°C, add water for injection to 1000ml, stir well, autoclave at 121°C and 15 pounds for 30 minutes, and store at 37°C after sterilization.

[0028] 3) Proportion according to the volume ratio of liquid A: liquid B = 1:1, and mix well to get the heat-resistant freeze-drying protective agent.

[0029] The lyoprotectant prepared by the invention has undergone long-term...

Embodiment 2

[0038] 1. Preparation of lyoprotectant:

[0039] 1) Preparation of liquid A: Weigh 80g of NZ-amine, 10g of monopotassium glutamic acid, 500g of sucrose, and 50g of hydrolyzed milk protein; dissolve the above ingredients in 800ml of water for injection in order, shake well after fully dissolving, and add water for injection to 1000ml, sterilized by filtration with a 0.22μm filter membrane, and stored in a 37°C greenhouse for future use.

[0040] 2) Preparation of liquid B: Take 120g of gelatin, fully dissolve it with 800ml of water for injection at 70-80°C, add water for injection to 1000ml, autoclave at 121°C and 15 pounds for 30 minutes, and store at 37°C after sterilization.

[0041] 3) According to the ratio of liquid A: liquid B = 1:1, it can be used as a heat-resistant protective agent after mixing evenly.

[0042] 2. Vaccine preparation

[0043] 1. The cultured swine fever virus was tested for sterility and virus content, and the qualified virus solution was mixed with...

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Abstract

The invention relates to a lyophilized vaccine for classical swine fever. The lyophilized vaccine consists of classical swine fever virus serving as an antigen, and a protective agent, wherein the protective agent is prepared from the following substances in percentage by mass: 2.4-4% of NZ-amine, 0.3-0.5% of mono-potassium glutamate, 16-20% of sucrose, 6-20% of lactoalbumin hydrolysate, 1-1.6% of gelatin and the balance of water. According to the vaccine disclosed by the invention, the used lyophilized protective agent is made from raw materials which are low in cost, and the operation is simple, so that the vaccine can be produced in a large scale. After the vaccine prepared from the protective agent is stored for 24 months at the temperature of 2-8 DEG C, the protective rate of the vaccine can still reach over 90%, so that the storage life of the vaccine is prolonged effectively.

Description

technical field [0001] The invention belongs to the technical field of veterinary vaccines, in particular to a freeze-dried vaccine for swine fever. Background technique [0002] Classical swine fever (ClaSSiealSwinefever, CSF) is a highly contagious, fatal infectious disease of pigs caused by CSFV (ClaSSiealSwinefever virus, CSFV). The only natural host of the disease is pigs, which is one of the most serious infectious diseases that endanger the pig industry, and the mortality rate can reach more than 90% (Yin Zhen, Liu Jinghua, Animal Virology (First Edition) fMI, Beijing, Science Press , 1997, 652-653). [0003] In the 1950s, when various countries developed relatively ideal inactivated vaccines, Chinese scholars such as Zhou Taichong and others studied the Chinese line (C series) rabbit-like attenuated swine fever vaccine, the Japanese cell attenuated vaccine, and the French Thiverval strain. It was successful and played an important role in the control of swine fever...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/187A61K47/42A61K47/18A61K47/26A61P31/14
Inventor 胡潇邹桂荣李芳王龙张维孙健
Owner YEBIO BIOENG OF QINGDAO
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