Method for enhancing soil waterflooding effect to control pepper phytophthora blight

A technology for pepper blight and flooding, which is applied in botanical equipment and methods, chemicals for biological control, animal repellents, etc. The effect of simple operation, simple application process and low input cost

Active Publication Date: 2014-07-23
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to: in view of the production practice of high multiple cropping index, severe heavy cropping, widespread and serious occurrence of pepper blight in my country's protected pepper planting, the existing prevention and control measures are low in effectiveness, unstable or high in cost, and difficult to operate. However, it is still difficult to popularize and apply it on a large scale in actual production. It is proposed that the soil should be flooded for a certain period of time before adding Hartz wood with fast growth, easy cultivation, strong soil colonization ability and broad-spectrum disease prevention function. mildew, improve the stability and control effect of pepper blight prevention and control with simple operation method and low input cost

Method used

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  • Method for enhancing soil waterflooding effect to control pepper phytophthora blight
  • Method for enhancing soil waterflooding effect to control pepper phytophthora blight
  • Method for enhancing soil waterflooding effect to control pepper phytophthora blight

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Preparation of Trichoderma Harzianum Culture Medium

[0022] The Trichoderma harzianum used in this example was screened by the Jiangsu Academy of Agricultural Sciences. The strain was preserved in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures on May 24, 2013. The address of the preservation unit is: Beijing No. 3, No. 1, Beichen West Road, Chaoyang District, Institute of Microbiology, Chinese Academy of Sciences, Zip code 100101, and the preservation number is CGMCC No.7640.

[0023] Preparation of test tube slant medium: 100 grams of potatoes, 10 grams of glucose or sucrose, 10 grams of agar, 500 ml of water, and natural pH. Peel the potatoes, cut into pieces and boil for half an hour, then filter with 2 layers of gauze, add sugar and agar, heat and boil until the agar melts, make up the volume to 500ml, cool to about 60°C and divide into test tubes, the filling volume is 5 ml, plug the test tube mouth with a test tub...

Embodiment 2

[0027] Trichoderma harzianum ( Trichoderma harzianum ) Preparation of solid microbial agent (various media involved in this example are provided by Example 1)

[0028] A) Activation of strains

[0029] The method of activating the strains preserved on the slant of the test tube: Pick a piece of mycelium with a diameter of about 5mm from the strains preserved on the slant of the test tube and inoculate it into the PDA culture medium on the slant of the test tube for activation. After cultivating in an environment of 28±2°C for 3-5 days spare.

[0030] The method of activating the strains preserved by freeze-drying in ampoule tubes: scrub the ampoule with 70% alcohol cotton balls in an ultra-clean workbench, then make a groove in the ampoule with a grinding wheel, and use a sterile gauze pad or Wrap the ampoule with a sterile towel, then break the ampoule tube by hand and add 0.5-1.0ml of liquid medium PDB to it, slowly rotate the ampoule to rehydrate the freeze-dried bacteri...

Embodiment 3

[0037] Growth inhibition test of Trichoderma harzianum on Phytophthora capsici

[0038] Test method: Trichoderma harzianum and Phytophthora capsici were separately inoculated on PDA plates, and after 3 days of constant temperature cultivation at 30°C, fresh mycelium blocks (5 mm in diameter) were obtained from each strain, and Trichoderma harzianum and Phytophthora capsici were inoculated separately. The silk pieces were inoculated into the same PDA plate (each plate was inoculated with 1 Trichoderma harzianum mycelium piece and 1 Phytophthora capsici mycelium piece), and the distance between the two mycelium pieces was 5 cm, and they were cultured in a 30°C incubator Observed.

[0039] See the test results figure 1 , as can be seen from the figure, the described Trichoderma harzianum has obvious growth inhibitory effect on Phytophthora capsici, and the described Trichoderma harzianum mainly inhibits or kills the pathogenic bacteria through hyperparasitism, that is, by secret...

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Abstract

The invention relates to a method for preventing and controlling pepper blight by enhancing the effect of soil flooding. cm, surround the field with soil around the soil ridge, and flood the field with irrigation water for 12-15 days. During the flooding period, the surface of the field soil layer should be kept at least 5 cm deep; after the flooding is over, the soil should be dried naturally Apply the base fertilizer, then evenly sprinkle the Trichoderma harzianum solid inoculum, and plant peppers after the soil is rotated; the Trichoderma harzianum solid inoculum is prepared from the Trichoderma harzianum (Trichoderma harzianum) strain with the preservation number CGMCC No.7640. The bacteria content in the solid inoculum of Trichoderma harzianum is 1010-11cfu / g; the dosage of the solid inoculum of Trichoderma harzianum is 30-40 kg / mu.

Description

technical field [0001] The invention relates to a method for enhancing the effect of soil flooding to prevent and control pepper blight. Specifically, the field soil is flooded for a certain period of time and then the fungus agent Trichoderma harzianum is applied to improve the effect of preventing and controlling pepper blight. The invention belongs to the field of comprehensive prevention and control of crop diseases by using soil control measures. Background technique [0002] Capsicum blight is a worldwide soil-borne disease that commonly occurs in various capsicum producing areas in the world. Leonian first reported in 1922 that the pathogen causing pepper blight was Phytophthora capsici. The pathogen caused severe disease in the pepper producing area in the autumn of 1918, and the disease continued to occur in the affected area one year later and spread to the surrounding areas. The occurrence of pepper blight is not restricted by geographical environment. It occur...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/00A01G13/00A01N63/04A01P3/00
Inventor 马艳常志州王光飞徐跃定张建英
Owner JIANGSU ACAD OF AGRI SCI
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