Micro-fluidic serum extracting chip

A microfluidic and serum technology, applied in the direction of laboratory equipment, test sample preparation, chemical instruments and methods, etc., can solve the problems of blood sample loss, complex mechanical structure, interference of spectral analysis, etc., and achieve simple operation, separation high efficiency effect

Inactive Publication Date: 2013-09-11
苏州扬清芯片科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, blood testing is the most widely used sample testing in biomedicine, clinical diagnosis, health and quarantine diagnosis, but the presence of red blood cells has a great interference on the spectral analysis in blood testing, so how to quickly and easily separate serum from whole blood It has always been a hot and difficult issue in blood testing research
[0003] At present, most hospitals and laboratories still use large centrifuges to separate serum and blood cells. This method requires blood samples in milliliters to be drawn from patients, but the samples actually used for testing are only tens or even several microliters, resulting in A large amount of waste; and this kind of centrifuge equipment is large in size and complex in mechanical structure. The serum separation process and detection are carried out separately and independently, which is easy to cause the loss and contamination of blood samples. It is not suitable for the integration of clinical diagnosis and micro-analysis systems, and cannot meet medical The need for the development of micro-analysis system in diagnosis, so it is necessary to develop a new micro-separation technology

Method used

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  • Micro-fluidic serum extracting chip

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Effect test

Embodiment 1

[0015] Embodiment 1 A polymethyl methacrylate serum extraction chip

[0016] Such as figure 1 As shown, a kind of serum extraction chip provided by the present invention is made up of sampling pool A, microchannel B, serum separation chamber C, and sample collection pool D, wherein the serum separation chamber is filled with two kinds of glass beads (particle diameter 20 μm and 100 μm, respectively). The chip material is polymethyl methacrylate, and the microstructures are precisely processed by CNC machine tools. The width and depth of the microchannels are 80 μm, and the chips are finally sealed by hot pressing.

[0017] The serum separation steps are as follows: add whole blood from the sample pool, the blood sample flows through the serum separation chamber under capillary force or a certain negative pressure, blood cells are trapped at the front end, and serum flows out from the pores between the microbeads, and finally enters the chamber. Sample collection pool. Using...

Embodiment 2

[0018] Embodiment 2 A kind of polydimethylsiloxane serum extraction chip

[0019] Such as figure 1 As shown, a kind of serum extraction chip provided by the present invention is made up of sampling pool A, microchannel B, serum separation chamber C, and sample collection pool D, wherein the serum separation chamber is filled with two kinds of glass beads (particle diameter 15 μm and 120 μm, respectively). The chip material is polydimethylsiloxane, and the microstructure is processed by casting polymerization method based on photolithography mold. The width and concentration of the microchannel are 100 μm, and finally irreversible sealing is adopted.

[0020] The serum separation steps are as follows: add whole blood from the sample pool, the blood sample flows through the serum separation chamber under capillary force or a certain negative pressure, blood cells are trapped at the front end, and serum flows out from the pores between the microbeads, and finally enters the cham...

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Abstract

The invention aims to provide a micro-fluidic serum extracting chip. The chip is formed by a blood sample introduction pool, a micro channel, a serum separation chamber and a sample collecting pool, wherein the interior of the serum separation chamber is filled with two kinds of micro balls with different hole diameters. The operating principle of the chip is as follows: the whole blood is added from a sample introduction port, the blood sample flows through the serum separation chamber along the micro channel under the action of surface tension or certain negative pressure, hemocytes are intercepted by a micro ball structure, and the serum flows out from ventages among the micro balls, and finally enters the sample collecting pool. The chip provided by the invention can be used for rapidly extracting the serum on line, the operation is simple and convenient, the separation efficiency is high, and the further integration with other operation units is easy to realize.

Description

technical field [0001] The invention relates to a microfluidic chip, in particular to a serum extraction chip. Background technique [0002] Biochemical medical testing based on human body fluids such as blood, urine and saliva can provide important information that reflects people's health status. At present, blood testing is the most widely used sample testing in biomedicine, clinical diagnosis, health and quarantine diagnosis, but the presence of red blood cells has a great interference on the spectral analysis in blood testing, so how to quickly and easily separate serum from whole blood It has always been a hot and difficult issue in blood testing research. [0003] At present, most hospitals and laboratories still use large centrifuges to separate serum and blood cells. This method requires blood samples in milliliters to be drawn from patients, but the samples actually used for testing are only tens or even several microliters, resulting in A large amount of waste; ...

Claims

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Application Information

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IPC IPC(8): B01L3/00G01N1/34
Inventor 叶嘉明
Owner 苏州扬清芯片科技有限公司
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