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Preparation method of lactobacillus acidophilus

A technology of Lactobacillus acidophilus and organic acid, which is applied in the field of preparation of Lactobacillus acidophilus, can solve the problems of Lactobacillus acidophilus unsatisfactory, needs to be improved, and single product, so as to improve the function of gastrointestinal tract and facilitate industrial implementation , the effect of simple method

Inactive Publication Date: 2013-08-21
XIAMEN LIUWEI BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The existing preparation method of Lactobacillus acidophilus has a single product, and at the same time only pays attention to the growth of the number of viable Lactobacillus acidophilus. Unsatisfactory
[0004] Therefore, the current process still needs to be improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: liquid fermentation

[0020] 1. Medium components: 2% by weight of glucose, 1% by weight of peptone, 1% by weight of beef extract, 0.5% by weight of yeast extract, 0.2% by weight of dipotassium hydrogen phosphate, 0.5% by weight of NaAc, 0.05% by weight of magnesium sulfate, 0.025% by weight of manganese sulfate, 0.1% by volume of Tween-80, 0.2% by weight of diamine citrate, 1.5% by weight of agar, and 5% by weight of tomato juice.

[0021] 2. Inoculation amount: 3% Lactobacillus acidophilus, from the School of Food and Bioengineering, Zhejiang Gongshang University, preserved in CGMCC, preservation number CGMCC No.2122, patent number ZL200710156323.X (a Lactobacillus acidophilus, The method for fermenting soybean milk with the strain and fermenting soybean milk beverage), the disclosure date is May 21, 2008.

[0022] 3. Temperature: 30°C

[0023] 4. The pH value of the fermentation process: not less than 6-6.5

[0024] 5. Fermentation time: 24 hours for ...

Embodiment 2

[0025] Example 2: solid fermentation

[0026] 1. Medium components: brown sugar 1%, corn flour 50%, bran 42.3%, soybean meal 5%, magnesium sulfate 0.5%, ammonium sulfate 0.5%, dipotassium hydrogen phosphate 0.5%, Tween-80 0.2%

[0027] 2. Inoculum size: 50% (using the fermented liquid obtained in Example 1)

[0028] 3. Fermentation method: closed anaerobic fermentation

[0029] 4. Fermentation process temperature: 30-35°C

[0030] 5. Fermentation time: 96 hours

Embodiment 3

[0031] Example 3: Drying and testing

[0032] Low-temperature boiling drying is adopted, and the drying temperature is not higher than 40°C.

[0033] Example 1 uses a 1000-liter fermenter, the final output is 818 kg (weighed after direct drying), and the yield is 97.5%. (Yield rate = final product weight * 100% / (800+ liquid medium dry matter weight))

[0034] Detection method:

[0035] 1. Detection of viable Lactobacillus acidophilus: the total number of viable bacteria is detected by the method specified in GB / T 20191-2006, and the pH value is detected by a pH meter. The total number of viable Lactobacillus acidophilus is greater than or equal to 2 billion / g, and the pH value is 4-5.

[0036] 2. Lactobacillus detection: The qualitative and quantitative detection of lactobacillus is carried out by reversed-phase high-performance liquid chromatography. The activity of lactobacillus in the product can reach 75WU / mL.

[0037] 3. Organic acid detection: use high-performanc...

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Abstract

The invention provides a preparation method of lactobacillus acidophilus. The preparation method comprises the steps of: inoculating lactobacillus acidophilus of CGMCC No.2122; performing liquid state fermentation; performing solid fermentation; drying; and drying. The lactobacillus acidophilus prepared by the method is accompanied with other beneficial metabolites comprising lactein and organic acids such as L-lactic acid, n-butyric acid, acetic acid, formic acid and the like. The products are important energy sources of animal intestinal tract and can cooperate with probiotics to act on animals, so that the gastroenteric function of the animals can be obviously improved, the immunity is improved and growth is promoted. The products prepared by the method are used for preparing feed, so the digestion and absorption rate of the animals on the feed is increased.

Description

technical field [0001] The invention relates to the field of fermentation engineering, in particular to a preparation method of Lactobacillus acidophilus. Background technique [0002] Lactobacillus acidophilus is not only in the stomach, but also the main probiotic in the human small intestine, which can adjust the balance of intestinal flora and inhibit the proliferation of harmful intestinal microorganisms. [0003] The existing preparation method of Lactobacillus acidophilus has a single product, and at the same time only pays attention to the growth of the number of viable Lactobacillus acidophilus. Unsatisfactory. [0004] Therefore, the current technology still needs to be improved. Contents of the invention [0005] The purpose of the present invention is to provide a preparation method capable of preparing excellent Lactobacillus acidophilus and a large number of other beneficial metabolites. [0006] In order to achieve the above object, the technical solution...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P1/04C12P7/56C12P7/52C12P7/54C12P7/40C12R1/23
Inventor 廖庄旺
Owner XIAMEN LIUWEI BIO TECH
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