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Preparation method for steelhead trout and arctic char hybrid triploid seed

A steelhead trout and triploid technology is applied in the field of preparation of steelhead trout and arctic charr hybrid triploid seedlings, which can solve the problem of high water quality requirements in the growth environment, sensitivity to chemical substances, lack of 6-2 Methylaminopurine etc.

Inactive Publication Date: 2014-12-10
BEIJING FISHERIES RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] 6-Dimethylaminopurine (N6-Dimethyladenine, 6-DMAP) has achieved good results in the induction of polyploidy in various shellfish, but because salmon trout have high requirements on the water quality of the growth environment and are particularly sensitive to chemical substances, So far, there is no successful report on the application of 6-dimethylaminopurine in the induction of triploid or hybrid triploid in salmon and trout

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1 Preparation method of hybrid triploid seed of steelhead trout and arctic char (1)

[0028] Stimulation by water flow, light control, and enhanced compound feed for broodstock were used to make broodstock of steelhead trout and broodstock of arctic charr develop and mature synchronously. The water temperature for broodstock breeding of steelhead trout was 10°C, and the water exchange rate in the running pool was 2h / time; the broodstock breeding water temperature for Arctic char was 8°C, and the water exchange volume in the running pool was 5h / time.

[0029] The eggs are fertilized by dry method. Squeeze the roe of mature steelhead female fish into a clean porcelain basin first, and then squeeze the semen of 2 male arctic char into a clean porcelain basin. The water temperature is 8°C, stir evenly immediately, add hatching water with a volume ratio of semen to hatching water of 1:2500, stir gently, pour out the water after 1 min, repeat 3 times, clean the se...

Embodiment 2

[0032] Example 2 Method for preparing triploid hybrid seed of steelhead trout and arctic char (2)

[0033] Stimulation by water flow, light control, and enhanced compound feed for broodstock were used to make steelhead trout and arctic char broodstock mature synchronously. The water temperature for broodstock breeding of steelhead trout was 13°C, and the water exchange rate in the running pool was 2h / time.

[0034] The eggs are fertilized by dry method. Squeeze the roe of mature steelhead female fish into a clean porcelain basin first, and then squeeze the semen of 2 male arctic char into a clean porcelain basin. The water temperature is 6°C, stir evenly immediately, add hatching water with a volume ratio of semen to hatching water of 1:3000, stir gently, pour out the water after 1.5 minutes, repeat 3 times, clean the semen, and place it for treatment.

[0035] After 13 minutes of fertilization, the fertilized eggs were quickly moved into the 6-DMAP solution with a concentra...

Embodiment 3

[0037] Embodiment 3 Preparation method of hybrid triploid seed of steelhead trout and arctic char (3)

[0038]Stimulation by water flow, light control, and enhanced compound feed for broodstock were used to make steelhead trout and arctic char broodstock mature synchronously. The water temperature for broodstock breeding of steelhead trout was 11°C, and the water exchange rate in the running pool was 2h / time.

[0039] The eggs are fertilized by dry method. Squeeze the roe of mature steelhead female fish into a clean porcelain basin first, and then squeeze the semen of 2 male arctic char into a clean porcelain basin. The water temperature is 7°C, stir evenly immediately, add hatching water with a volume ratio of semen to hatching water of 1.5:3000, stir gently, pour out the water after 1 min, repeat 3 times, clean the semen, and place it for treatment.

[0040] After 14 minutes of fertilization, the fertilized eggs were quickly transferred to the 6-DMAP solution with a concen...

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Abstract

The invention provides a preparation method for a steelhead trout and arctic char hybrid triploid seed. The preparation method includes the steps that steelhead trout female broodstock egg and arctic char male broodstock sperm are in vitro fertilized; after the fertilized egg is fertilized for 12-14 minutes, the fertilized egg is moved into 6-DMAP (dimethylanminopyridine) solution with concentration of 170-190 milligrams per liter and temperature of 9-11 degrees Celsius to be soaked for 14-16 minutes; and then the soaked fertilized egg is moved into water of the temperature of 6-8 degrees Celsius to be hatched. After about 50 days, a hybrid triploid larvae is hatched by the fertilized egg. During incubation period, eyed rate of the fertilized egg is above 81%, and hatching rate of the seed reaches above 90%. Then the obtained larvae is detected through a flow cytometry, and triploid rate of the obtained larvae reaches 94%. With the preparation method for the steelhead trout and the arctic char hybrid triploid seed, during trout breeding process, problems of precocious puberty, individual miniaturization, disease breeding and the like in an individual fish are alleviated. Furthermore, the preparation method has the advantages of hybrid and triploid and has good market value.

Description

technical field [0001] The invention relates to the technical field of aquatic organism breeding, in particular to a preparation method for hybrid triploid seed of steelhead trout and Arctic char. Background technique [0002] Salmon trout is one of the main fish cultured in the world at present. Because of its own quality and special requirements for the living environment, it is a green, environmentally friendly and nutritious food, so it is very popular among consumers. Salmon trout is currently the main cold-water cultured fish in my country. With the rapid development of salmon trout farming in China, basic theoretical research is relatively lagging behind. Problems such as the degradation of broodstock germplasm of salmon trout lead to the degradation of seed quality and the decline in production efficiency have gradually become prominent. , thus restricting the sustainable development of salmon and trout farming. Using modern biotechnology to cultivate seedlings with ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 徐绍刚李文通田照辉杨晓飞杨贵强
Owner BEIJING FISHERIES RES INST
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