Process for synthesizing and preparing mildew oxide by mildew fermentation organisms and used novel nitrogen source

Abstract

The invention discloses a process for synthesizing and preparing mildew oxide by steroid hormone drug mildew fermentation and a needed novel nitrogen source. The process for synthesizing and preparing mildew oxide by mildew fermentation organisms comprises the following steps of: stirring and reacting under 24-30 DEG C by matching with the novel nitrogen source; ventilating in a biological fermentation process; carrying out oxidation reaction for 24-72 hours; filtering by a plate frame in a pressed manner for dehydrating and drying; converting substrate W-oxide into mycelium containing the mildew oxide; extracting by acetone to obtain a mildew oxide crude product; refining by a mixed solvent of toluene and chloroform to obtain the mildew oxide fine product. The formula of the novel nitrogen source is more suitable for the biological fermentation process requirements, so that the yield is 92% or higher, the product stability is stable, the yield is high, the indexes associated with the conversion rate and the yield are high and the economic benefits are considerable, and therefore, the process is suitable for the industrial production.

Description

technical field [0001] The invention relates to the field of mold fermentation of steroid hormone drugs, in particular to a process for preparing mold oxides by mold fermentation biosynthesis and a required new nitrogen source. Background technique [0002] Epoxy progesterone is white crystalline powder, odorless, soluble in methanol, toluene and other organic solvents. It is produced from natural steroidal diosgenin through ring-opening, acetylation, oxidation, hydrolysis, elimination, epoxidation, oxidation and other reactions. It is cheap and easy to get. It is a very important class of steroid drug intermediates that are used in the largest amount in my country. It is widely used in the synthesis of adrenocortical hormone drugs, and is the main raw material of steroid hormone drugs such as hydrocortisone, cortisone, prednisone, dexamethasone and fluocinolone. [0003] In 1952, Murray et al. of U.S. General Pharmaceutical Company used Rhizopus niger to convert progesteron...

AI Technical Summary

Problems solved by technology

[0006] Aiming at the above deficiencies, the present invention provides a process for preparing mold oxides by mold fermentation and biosynthesis and the required new nitrogen source. It is an improvement for the deficiencies in the prior art. It is a biological fermentation process with defects such as low amino acid ratio and unreasonable amino acid ratio; it is a major breakthrough in greatly improving product conversion rate and yield index under the premise of ensuring the quality of existing products, which cannot be achieved by various improvements over the years of

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