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Application of panax japonicus saponin IV to preparation of antilipemic agent

A technology of bamboo ginseng saponin and blood lipid lowering, which is applied in the direction of drug combination, pharmaceutical formula, steroids, etc., can solve problems such as rhabdomyolysis, pain, adverse reactions, etc., and achieve the effect of increasing HDL-C content and lowering blood fat. , the effect of broad application prospects

Active Publication Date: 2013-07-24
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Statins inhibit liver cholesterol synthesis by inhibiting the rate-limiting enzyme HMG-CoA reductase in the early stage of intracellular cholesterol synthesis, reduce intracellular free cholesterol, and then feedback up-regulate the expression of low-density lipoprotein (LDL) receptors on the cell surface , increase the number and activity of LDL receptors in cells, accelerate the clearance of low-density lipoprotein cholesterol (LDL-C) in the blood, thereby reducing blood lipids; but statins have serious adverse reactions, which can cause liver damage and cause transaminase elevation High, muscle pain, elevated serum creatine muscle enzyme (CK), severe cases can cause rhabdomyolysis, acute renal failure

Method used

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  • Application of panax japonicus saponin IV to preparation of antilipemic agent
  • Application of panax japonicus saponin IV to preparation of antilipemic agent
  • Application of panax japonicus saponin IV to preparation of antilipemic agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: Preparation, structure identification and purity determination of bamboo ginseng saponin IV

[0036] Preparation of bamboo ginseng saponin Ⅳ compound:

[0037] Medicinal material extraction: take the bamboo ginseng medicinal material, crush it, add 10 times the mass concentration of the bamboo ginseng medicinal material, soak in 90% ethanol for 2 hours, heat and reflux for extraction for 2 hours, filter to obtain the filtrate, and use 9 times the medicinal residue of the bamboo ginseng medicinal material respectively The mass concentration is 90% ethanol, reflux, extract for 2 hours, 2 hours, filter to obtain filtrates respectively, combine three times of filtrates, recover ethanol under reduced pressure until there is no alcohol smell, dilute with water to 0.1g crude drug / ml, this is macropore Loading solution for resin column enrichment;

[0038] HPD722 macroporous resin column enrichment of the extract: Take 200ml of the above-mentioned 0.1g crude drug / ml...

Embodiment 2

[0052] Example 2: Preparation of Bamboo Ginseng Saponin IV

[0053] Preparation of bamboo ginseng saponin Ⅳ compound:

[0054] Medicinal material extraction: take the bamboo ginseng medicinal material, crush it, add 12 times the mass concentration of the bamboo ginseng medicinal material, soak in 90% methanol for 2 hours, heat and reflux for extraction for 3 hours, filter to obtain the filtrate, and use 9 times the medicinal residue of the bamboo ginseng medicinal material respectively Quantity, 10 times the mass concentration of 90% methanol reflux extraction for 2h, 2h, filtered to obtain filtrates respectively, combined three filtrates, recovered methanol under reduced pressure until no alcohol smell, diluted with water to 0.1g crude drug / ml, this is macropore Loading solution for resin column enrichment;

[0055] HPD722 macroporous resin column enrichment of the extract: Take 200ml of the above-mentioned 0.1g crude drug / ml sample solution and add it to the HPD722 macropor...

Embodiment 3

[0058] Example 3 Lipid-lowering experiment in vitro of bamboo ginseng saponin Ⅳ

[0059] 1. Preparation of sample administration solution

[0060] Precisely weigh 0.41 mg of bamboo ginseng saponin IV (prepared according to the method in Example 1) with an electronic balance of 1 / 100,000 in a 15 ml centrifuge tube, add 10 ml of high-sugar DMEM medium to dissolve, and obtain bamboo ginsenoside IV with a concentration of 41 μg / ml. Ginsenoside IV sample solution.

[0061] 2. Cell Seeding

[0062] Take the well-growing cells covering 80-90% of the bottom of the culture flask, digest them with 0.25% trypsin into a cell suspension and count them, adjust the cell concentration to about 70,000 cells / ml, inoculate in a 24-well plate, add 1ml of cell suspension means about 70,000 cells per well. Place the inoculated cells in an incubator to continue incubation.

[0063] 3. Experimental grouping

[0064] After inoculating the 24-well plate with cells for 24 hours, take it out, discar...

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Abstract

The invention discloses an application of panax japonicus saponin IV to preparation of an antilipemic agent. Based on a pharmacodynamic experiment, the panax japonicus saponin IV can remarkably reduce the contents of TG (triglyceride), TC (total cholestero) and LDL-C (low-density lipoprotein cholesterol) in blood serum of a mouse with low yolk hyperlipidemia disease, and increases the content of HDL-C (high-density lipoprotein cholesterol); and the safety is high, the antilipemic function is ensured, and a new choice is provided for clinic treatment of the hyperlipidemia disease.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine, and in particular relates to the application of bamboo ginseng saponin IV in the preparation of blood lipid-lowering medicine. Background technique [0002] Hyperlipidemia is a very dangerous disease that can lead to coronary heart disease and stroke; the prevalence is high, the global prevalence of hyperlipidemia is 7.6%, the prevalence in the United States is as high as 50%, and the prevalence in China is as high as 30%. The number of patients in China alone is as high as 160 million; the prevalence of hyperlipidemia is increasing year by year, and the investment in treatment is huge. [0003] The existing clinical first-line lipid-lowering drugs are mainly statins (simvastatin, etc.). Statins inhibit liver cholesterol synthesis by inhibiting the rate-limiting enzyme HMG-CoA reductase in the early stage of intracellular cholesterol synthesis, reduce intracellular free chol...

Claims

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Application Information

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IPC IPC(8): A61K31/704A61P3/06C07J63/00
Inventor 杨中林祁东利
Owner CHINA PHARM UNIV
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