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Streptomyces trehalose synthase gene and application thereof

A technology of trehalose synthase and trehalose, which is applied in the field of genetic engineering and modern enzyme engineering, can solve the problems of different synthetic pathways, different sources of microbial strains, and great differences in the coding DNA sequence and amino acid sequence of trehalose synthase, etc. To achieve the effect of fast response and market competitiveness

Inactive Publication Date: 2013-07-17
GUANGXI UNIV
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Problems solved by technology

[0016] However, there is no literature report about cloning trehalose synthase gene from Streptomyces, and the trehalose synthesis pathways of these trehalose synthesis-related enzymes are different, or the sources of microbial strains are different, resulting in the coding DNA sequence of trehalose synthase and The amino acid sequences are quite different, and the molecular weights, enzymatic properties, and substrate conversion rates of the enzymes are all different, and most of the trehalose synthase genes have not yet been applied on an industrial scale

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  • Streptomyces trehalose synthase gene and application thereof
  • Streptomyces trehalose synthase gene and application thereof
  • Streptomyces trehalose synthase gene and application thereof

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[0039] The technical scheme of the present invention is described further below by embodiment, following experiment and example described content belong to main content of the present invention, but are not limited to these content, although some extension content obvious to those skilled in the art does not appear in this specification, but should also belong to the scope of patent claims of the present invention.

[0040] 1. Streptomyces culture

[0041] Streptomyces sp GXT6, whose strain number is CICC 11021, is stored in China Industrial Microorganism Culture Collection Center. Streptomyces is inoculated in the following medium (g / L): 3g of yeast extract, 5g of peptone, 3g of malt extract, and 10g of glucose , 340g sucrose, add distilled water to 1L, sterilize at 120°C for 20min, add 2ml.2.5mol / L MgCl after autoclaving 2 Solution, shake on a 45-50°C constant temperature shaker for 40 hours after inoculation, and then collect the bacteria for total DNA extraction.

[004...

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Abstract

The invention provides a Streptomyces trehalose synthase gene and application thereof. The gene is cloned from Streptomyces sp GXT6; trehalose synthase expressed by the gene can transform maltose into trehalose. The biggest characteristic of the gene is that a substrate has high transformation efficiency and a fast reaction speed; under optimal reaction conditions, a reaction product obtained in transformation of maltose into trehalose contains 86.68% of trehalose, only 3.56% of glucose is produced, so a transformation rate of the substrate is increased, and production cost is reduced. The trehalose synthase can be used in production of trehalose, and the trehalose can be used in fields like the food industry, cosmetics and pharmaceutical and biological products.

Description

technical field [0001] The invention relates to the technical field of genetic engineering and modern enzyme engineering, in particular to a streptomyces trehalose synthase gene and its application. Background technique [0002] Trehalose is a non-reducing disaccharide composed of two molecules of glucose connected by α,α-1,1 glycosidic bonds. It widely exists in microorganisms, shrimps, Saccharomyces cerevisiae, mushrooms and various fungi, insects, plants, etc. Exogenous trehalose also has a good non-specific protective effect on organisms, biological macromolecules, and biofilms. It can protect cells from damage caused by environmental changes (such as dehydration, hypertonicity), and the specific performance is Effective protection of biofilm, protein and DNA, etc., so it is known as "sugar of life". The content of trehalose in nature is very low. In the past, it was mainly extracted from dry yeast. Due to the low content, the extraction process is complicated and the c...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/61C12N9/90C12P19/24C12P19/12
Inventor 韦宇拓黄英谭惠敏杜丽琴梁甲元黄日波
Owner GUANGXI UNIV
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