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Reagent device and method for detecting varicella-zoster-virus antibody

A technology of herpes zoster virus and chickenpox, which is applied in the field of clinical immunology detection, can solve the problems of many types of reagent bottles, not suitable for laboratories, and insufficient objectivity of analysis results.

Active Publication Date: 2015-01-07
SHENZHEN YHLO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] (1) Non-specific recognition cannot be distinguished according to the size of the molecular weight when analyzing the results;
[0009] (2) The operation is relatively complicated and requires expensive fluorescence microscopes, which are difficult to popularize in many primary hospitals and are not suitable for laboratories with a large number of specimens;
[0010] (3) The background in the fluorescence measurement is relatively high, and it is difficult to use the fluorescence immunoassay technique for quantitative determination;
[0011] (4) Experienced professionals are required to determine the results, and the objectivity of the analysis results is insufficient;
[0012] (5) Only qualitative testing can be performed, not quantitative testing
[0015] (1) Use 12×8 type, 6×8 type, 8×12 type or whole plate type 96-well special microwell plate as antigen coating equipment and reaction Containers can only be divided into 12 batches, 6 batches, 8 batches or the whole board for one-time use, and independent and single-person testing cannot be carried out;
[0016] (2) There are many types of reagents used in the determination, and each detection reagent must be filled with a reagent bottle, and each time a reagent is used, it is necessary to replace the suction nozzle to add to the microwells of the microwell plate respectively, Not only are there many types of reagent bottles, but also the operation of filling reagents is extremely cumbersome;
[0017](3) There is a lack of corresponding labeling of the test information, and the production batch number and expiration date information of the test reagent can only be known or known by checking the label on the outer packaging box of the test kit, Moreover, the known information is not controlled during the detection process and has great randomness;
[0018](4) The detection reagent is in an open space during the detection process, which may easily cause cross-contamination among various reagents and affect the accuracy of the detection result;
[0019](5) The detection process is mostly manual operation, the addition of reagents or samples is not very precise, the operation process is extremely cumbersome and complicated, and operation errors are prone to occur. Poor accuracy and precision;
[0020](6) The quantity configuration and use of the complete set of reagents for the test items are the number of items × 48 / 96 servings. If 10 items need to be tested, the reagent’s The number of configuration and use must be 10×48 / 96. If only one sample needs to be tested for 10 different items, it is also necessary to configure 10×48 / 96 reagents, which is not economical and reasonable.

Method used

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  • Reagent device and method for detecting varicella-zoster-virus antibody
  • Reagent device and method for detecting varicella-zoster-virus antibody
  • Reagent device and method for detecting varicella-zoster-virus antibody

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0095] Example 1 Reagent device 1 for detecting varicella-zoster virus antibody

[0096] Such as Figure 1-2 As shown, the reagent device for detecting varicella-zoster virus antibody described in the application of the present invention is in the shape of a strip, including a base 10 with eight holes and a handle 20 located at one end of the base 10, and the sequence of the eight holes is Starting from the end near the handle 10, there are sample holes 11, auxiliary agent holes 12, enzyme conjugate holes 13, substrate holes 14, stop solution holes 15, diluent holes 16, reaction holes 17 and dilution holes 18. The sample The sample to be tested is contained in the hole 11, and the auxiliary agent hole 12 is used for adding auxiliary reagents when the detection needs (such as adding an adsorbent in the auxiliary agent hole when detecting IgM), adding the enzyme conjugate solution in the enzyme conjugate hole 13, and adding the enzyme conjugate solution to the substrate hole. Add...

Embodiment 2

[0099] Example 2 Reagent device 2 for detecting varicella-zoster virus antibody

[0100] Such as image 3 As shown, the reagent device for detecting varicella-zoster virus antibody in this embodiment has the same basic structure as that of the reagent device in Example 1, and the reagent device also includes several support columns 50, and the support The columns 50 are located under the base 10 in the reagent device, and there are more than one hole between adjacent support columns 50 . The function of the support columns 50 is to enhance the mechanical strength and balance of the base.

Embodiment 3

[0101] Example 3 Reagent device 3 for detecting varicella-zoster virus antibody

[0102] Such as Figure 4-6 As shown, it is a preferred embodiment of the reagent device for detecting varicella-zoster virus antibody described in the application of the present invention. Its basic structure is the same as that of Embodiment 1 or Embodiment 2, except that the reaction hole 17 is a detachable structure. , the reaction hole 17 is composed of an outer hole 171 and an inner hole 172, the bottom of the outer hole 171 is provided with a bottom hole 174, the inner hole 172 passes through the bottom hole 174 and closely fits with the bottom hole 174, between the outer hole 171 and the inner hole 172 An anti-overflow chamber 173 is left, and the function of the anti-overflow chamber 173 is that during the reaction, if the liquid overflows, it can stay in the chamber to prevent contamination of instruments and other reagent devices.

[0103] Further, the cooperating fixing method of the ...

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Abstract

The invention provides a reagent device and a method used for detecting varicella-zoster-virus antibodies. The reagent device has a long strip shape, and has a base body comprising 8 hole positions and a handle positioned on one end of the base body. From the end proximal to the handle, the 8 hole positions are sequentially a sample hole, an auxiliary agent hole, an enzyme conjugate hole, a substrate hole, a termination liquid hole, a dilution liquid hole, a reaction hole, and a dilution hole. According to the invention, based on a principle of enzyme-linked immunoassay, varicella-zoster-virus antibody is detected by using the reagent device. The method is an independent single-person analysis and detection method. The device can be used in cooperation with a corresponding specific analysis instrument. During a detection process, detection reagents or samples are injected by using a full-automatic precise dosing device. The device and the method have the advantages of automatic operation, precise dosing, high detection result accuracy, high detection result precision, and wide application prospect.

Description

technical field [0001] The application of the present invention relates to a reagent device and method for detecting varicella-zoster virus antibody, belonging to the technical field of clinical immunology detection. Background technique [0002] Varicella-Zoster virus (VZV) is spherical, with a diameter of 150nm to 200nm. The complete virus consists of a core, a nucleocapsid and an envelope. The core of the virus is double-stranded DNA with a small amount of enzyme protein. The core-shell consists of 162 hollow tubular shell particles to form a 20-hedron. The outermost layer is the envelope, which is composed of fat and glycoprotein, and the antigenic structure exists in the glycoprotein. [0003] Chickenpox is caused when children are infected with varicella-zoster virus for the first time, and the virus in the latent body relapses after being stimulated to cause shingles, which is more common in adults and the elderly. Chickenpox occurs in children and is usually self-...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/569G01N21/31
Inventor 胡德明刘清波何林阳辉
Owner SHENZHEN YHLO BIOTECH
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