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Method for establishing tree-shrew breast cancer model for slow virus by nipple injection

A lentivirus and breast cancer technology, applied in the field of making animal models of human diseases, can solve the problems of long time-consuming cycle, low induction rate, poor model consistency, etc., and achieve good model consistency, short latency time, and high occurrence efficiency. Effect

Active Publication Date: 2013-06-26
KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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AI Technical Summary

Problems solved by technology

Conventional breast cancer induction methods such as the chemical carcinogen DMBA take a long period of time, the induction rate is low, and the model consistency is poor

Method used

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Examples

Experimental program
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Effect test

Embodiment Construction

[0024] In order to make the present invention easier to understand, specific implementation examples of the present invention will be further described below.

[0025] 1. Construction of viral vectors

[0026] The polyoma intermediate T antigen (PyMT), EGFP lentiviral expression vector and packaging vector plasmids used in the examples of the present invention were provided by the collaborator Li Yi, Baylor College of Medicine, USA. The virus vector used is a conventional lentivirus vector, and the virus particles packaged in it can infect and integrate its gene into the genome of tree shrew mammary gland epithelial cells.

[0027] 2. Virus packaging and titer determination

[0028] HEK293T cells were co-transfected with PyMT, EGFP gene expression vector FU-CGW and packaging vectors pRev, pMDL and pVSVG respectively, and the fresh DMEM medium was replaced 24 hours after transfection, and the culture supernatant was collected 48 hours after transfection, and removed by low-spe...

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Abstract

The invention discloses a method for establishing a tree-shrew breast cancer model for slow virus by nipple injection. Slow virus is introduced to a tree-shrew breast duct by adopting a nipple injection technology, so that a cancer gene is over-expressed or an anti-cancer gene is knocked down after epithelial cells in a mediated duct are infected, and therefore, tree-shrew breast tumors are induced. According to the method for establishing a tree-shrew breast cancer model for slow virus by nipple injection disclosed by the invention, the breast tumors can be touched within two weeks after expressing the cancer gene PyMT; and moreover, the induction success rate is almost 100%, and the method is short in incubation period, strong in specificity, extremely high in induction rate, stable and convenient and simple to operate. The method disclosed by the invention can be used for quickly and efficiently establishing the tree-shrew breast cancer model.

Description

technical field [0001] The invention relates to the technical field of making animal models of human diseases, in particular to a method for establishing a tree shrew breast cancer model induced by lentivirus-mediated overexpression of oncogenes or knockdown of tumor suppressor genes. Background technique [0002] At present, breast cancer research is mainly based on breast cancer models established by rodents, but they cannot fully simulate human diseases because of their great differences from humans. Although non-human primates are the most ideal model animals, they are expensive. Tree shrews are second only to higher primates in terms of biological taxonomy, closer to humans than rodents, and are cheap. [0003] As early as 1966, Elliot et al first reported a case of spontaneous breast cancer in tree shrews in "Nature". Afterwards, there were no reports on tree shrew mammary gland tumor models at home and abroad. In the previous work of our research group, 5 cases of ...

Claims

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Application Information

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IPC IPC(8): C12N15/867C12N7/01A01K67/027
Inventor 陈策实夏厚军何保丽角建林
Owner KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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