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Biological active peptide modified nano-silver and preparation method and applications thereof

A bioactive peptide and nano-silver technology, which is applied in the field of bioactive peptide-modified nano-silver and its preparation, can solve the problems of difficult surface modification of peptides and metal nano-materials, and achieve drug efflux avoidance, enhanced effect, and high-efficiency anti-inflammatory effects. The effect of tumor activity

Inactive Publication Date: 2013-06-26
SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the prior art, the use of biologically active peptides to modify AgNP particles still has the problem that it is difficult to directly modify the surface of peptides and metal nanomaterials.

Method used

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  • Biological active peptide modified nano-silver and preparation method and applications thereof
  • Biological active peptide modified nano-silver and preparation method and applications thereof
  • Biological active peptide modified nano-silver and preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Synthesis and Characterization of Nanosilver (AgNP-TAT Nanoparticles) Modified by Membrane-penetrating Peptide TAT

[0043] (1) Synthesis of nano silver

[0044] Add 200 mg of polyvinylpyrrolidone K-30 (PVP, purchased from Sinopharm Chemical Reagent Co., Ltd.) and 5-10 ml of ultrapure water into a 25 ml round bottom flask, and stir for 30 min at room temperature to fully swell the PVP. Then slowly add 0.006mol / l silver nitrate (purchased from Sinopharm Chemical Reagent Co., Ltd.) solution 1-2ml and stir for 30min, then quickly inject 0.008mol / l newly prepared NaBH 4 (purchased from Sinopharm Chemical Reagent Co., Ltd.) 5-8ml, stirred rapidly for 60min to obtain nano silver (AgNP).

[0045] (2) Modification of nano silver

[0046] Take 2ml of the above-prepared AgNP and inject it into a 5ml EP tube, add 2-4mg / ml of sulfhydryl-containing penetrating peptide TAT((SH)CGGGYGRKKRRQRRR, and use the standard solid-phase synthesis method known in the art to pass thro...

Embodiment 2

[0057] Example 2 Synthesis of Nano Silver (AgNP-LMWP) Modified by Low Molecular Weight Protamine (LMWP) Penetrating Peptide

[0058] (1) The preparation of nano-silver is as described in Example 1.

[0059] (2) Low molecular weight protamine (LMWP) penetrating peptide modified nano silver

[0060] Take 2ml of the above-prepared AgNP and inject it into a 5ml EP tube, add 2-4mg / ml of sulfhydryl-containing penetrating peptide LMWP ((SH)VSRRRRRGGRRRR) (SEQ ID NO: 3) 1ml, the preparation of the penetrating peptide It can be obtained by solid-phase synthesis using a standard solid-phase synthesis method known in the art, or the polypeptide sequence can be obtained by digesting protamine, and then, by combining with 3-(2-pyridine dithio) Propionic acid-N-succinimide ester undergoes a condensation reaction and is activated by dithiothreitol (a disulfide bond reducing reagent) to bear a sulfhydryl group. Mix the two solutions evenly, react in a water bath at 37°C for 2.5 hours, and t...

experiment example 1

[0063]1. MTT (2-(4,5-dimethylthiazole-2)-2,5-diphenylbromoblue tetrazolium, trade name: thiazolium blue) method to determine the anti-tumor effect of AgNP-TAT in vitro

[0064] (1) Concentration-dependent inhibition experiment

[0065] B16, Hela, MCF-7, MCF-7 / ADR cells in the logarithmic growth phase (B16 cells (mouse melanoma cells) were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences, and HeLa (human cervical cancer) cells were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences. library, MCF-7 cells (human breast cancer cells) were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences, MCF-7 / ADR cells (human breast cancer drug-resistant cells) were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences) and diluted to a density of 1.5 × 10 4 The cell suspension of cells / ml was transferred to Corning’s 96 cell culture well plate, and 200 μl of cell suspension was added to each well, and cultur...

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Abstract

The invention relates to a biological active peptide modified nano-silver, wherein the biological active peptide is mercapto cell-penetrating peptide, and the biological active peptide modified nano-silver is formed by covalently connecting the mercapto cell-penetrating peptide to the surface of AgNP through Ag-S bonds. The biological active peptide modified nano-silver provided by the invention not only realizes an ability of an inorganic nano material AgNP penetrating a cell membrane, thereby greatly improving the inhibition effect on the growth of tumor cells, but also finds a new potential drug for tumor treatment.

Description

technical field [0001] The present invention relates to an anti-tumor nano-medicine and its preparation method and application, and more specifically relates to a biologically active peptide-modified nano-silver as well as its preparation method and application. The biologically active peptide-modified nano-silver of the invention is a nano-medicine capable of highly efficient cytotoxic effect on multidrug-resistant tumors. Background technique [0002] Malignant tumors are major diseases that endanger human health. According to reports, the annual incidence of cancer in my country is about 2.6 million, and the death toll is about 1.8 million. In the past 30 years, the death rate of cancer in China has increased by 80%, and it has become the first cause of death for urban and rural residents in China. Despite many attempts to treat cancer, no major breakthroughs have been made. At present, due to the widespread use of chemotherapy drugs, it has caused the multidrug resista...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/48A61K33/38A61P35/00
Inventor 黄永焯刘金华
Owner SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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