Broccoli leftover extractive and preparation method thereof
A technology for broccoli and leftovers, which is applied in the field of extracting sulforaphane and flavonoids, can solve the problems of high loss of effective components, complicated operation, environmental pollution, etc., and achieves improved operability, mild extraction conditions and broad market prospects. Effect
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Embodiment 1
[0014] 1. Pretreatment of broccoli scraps. After pulping the broccoli scraps, add 1.5 times the amount of enzymatic hydrolysis solution for 3 hours at a temperature of 45 degrees. Ultrasonic waves are assisted during the enzymatic hydrolysis process. The preparation method of the enzymatic solution is: dissolve 3 grams of compound enzyme It is obtained in 500ml of buffer solution with a pH value of 4.9, wherein the compound enzyme preparation consists of 68% cellulase mass fraction and the rest is pectinase.
[0015] 2. Extraction of broccoli sulforaphane and flavonoids. After enzymatic hydrolysis, continue to add 2.8 times the weight of broccoli scraps, 78% ethanol for reflux extraction for 3 hours, filter after extraction, then add 4 times the amount of drinking water for reflux extraction for 2.5 hours, reflux twice Microwaves are used to assist in the extraction process, the extracts obtained by two reflux extractions are combined, the alcohol is recovered under reduced p...
Embodiment 2
[0018] 1. Pretreatment of broccoli scraps. After pulping the broccoli scraps, add 4.5 times the amount of enzymatic hydrolysis solution at a temperature of 50 degrees for 6.8 hours of enzymolysis, and assist ultrasonic waves during the enzymatic hydrolysis process. The preparation method of the enzymolysis solution is: dissolve 13 grams of compound enzyme preparation It is obtained in 500ml of buffer solution with a pH value of 6.1, wherein the compound enzyme preparation consists of 53% cellulase mass fraction and the rest is pectinase.
[0019] 2. Extraction of broccoli sulforaphane and flavonoids. After enzymatic hydrolysis, continue to add 3.8 times the weight of broccoli scraps, 95% ethanol for reflux extraction for 4.2 hours, filter after extraction, then add 4.8 times the amount of drinking water for reflux extraction for 4.1 hours, reflux twice Microwaves are used to assist in the extraction process, the extracts obtained by two reflux extractions are combined, the al...
Embodiment 3
[0022] 1. Pretreatment of broccoli scraps. After pulping the broccoli scraps, add 3 times the amount of enzymatic hydrolysis solution for 5 hours at a temperature of 48 degrees. The preparation method of the enzymolysis solution is: dissolve 8 grams of compound enzyme preparation in 500ml of pH value of 5.6 It is obtained in buffer solution, wherein the compound enzyme preparation is composed of cellulase mass fraction of 58%, and the rest is pectinase.
[0023] 2. Extraction of broccoli sulforaphane and flavonoids. After enzymatic hydrolysis, continue to add 3.5 times the weight of broccoli leftovers, 85% ethanol for reflux extraction for 4 hours, filter after extraction, then add 4.5 times the amount of drinking water for reflux extraction for 3.5 hours, and combine twice The extracted extract was extracted, the alcohol was recovered under reduced pressure and continued to be concentrated to a paste, and then vacuum-dried to obtain the broccoli leftover extract.
[0024] T...
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