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Fluorescent sensor for determining serum protein concentration and manufacturing method thereof

A fluorescent sensor and serum protein technology, which is applied in the field of sensor preparation, can solve the problem of low selectivity of serum protein, and achieve the effects of good biocompatibility, long storage time, and wide detection range

Inactive Publication Date: 2013-06-19
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among these conventional methods, the fastest detection takes 5-10 minutes (ultraviolet absorption method), the highest detection sensitivity is 2 μg (Coomassie brilliant blue method), and the selectivity of several methods for the detection of serum proteins is not high

Method used

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  • Fluorescent sensor for determining serum protein concentration and manufacturing method thereof
  • Fluorescent sensor for determining serum protein concentration and manufacturing method thereof
  • Fluorescent sensor for determining serum protein concentration and manufacturing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Preparation of hydrotalcite precursor: weigh 5.128gMg(NO 3 ) 2 ·6H 2 O, 3.7513gAl(NO 3 ) 3 9H 2 O and 6.006g of urea were dissolved in 100ml of deionized water to prepare a salt solution, then transferred to a high-pressure reactor, reacted at 110°C for 12h, centrifuged and washed the product, and the chemical formula was Mg 2 Al(OH) 6 (CO 3 )·6H 2 Hydrotalcite of O; take 1g of hydrotalcite and add 200ml to remove CO 2 In the water, stir for 1h to swell, then add sodium nitrate solution and stir for 0.5h, the sodium nitrate solution is 127gNaNO 3 Dissolve in 300ml to remove CO 2 prepared in water; then slowly add 350 μl concentrated nitric acid with a pipette gun, stir for 48 hours, and centrifuge twice with ethanol to obtain Mg 2 Al(OH) 6 (NO 3 )·6H 2 O hydrotalcite precursor;

[0027] 2. Take the Mg prepared in step 1 2 Al(OH) 6 (NO 3 )·6H 2 O hydrotalcite precursor 0.10g, add in the flask that 100ml formamide is housed, under N2 Stirring and reac...

Embodiment 2

[0044] 1. Same as implementation case 1;

[0045] 2. Take the Mg prepared in step 1 2 Al(OH) 6 (NO 3 )·6H 2 O hydrotalcite precursor 0.11g, add in the flask that 110ml formamide is housed, under N 2 Stirring and reacting for 45 hours under protection, centrifuging to obtain the hydrotalcite laminate colloid solution after peeling;

[0046] 3. The preparation concentration is 2×10 -5 The aqueous solution of erythrosin B sodium salt of mol / L is adjusted to pH 7.5 with NaOH, and stored in a volumetric flask in the dark;

[0047] 4. Take a 3cm×1cm quartz plate and use concentrated H with a volume ratio of 7:3 2 SO 4 and H 2 o 2 Wash with the mixed solution of ethanol, and then ultrasonically use ethanol for 3 times, each time for 20 minutes, to obtain a quartz sheet rich in hydroxyl groups on the surface, and then place it in the hydrotalcite laminate colloid solution prepared in step 2 for 20 minutes for pre-assembly, take it out and use Rinse with deionized water and d...

Embodiment 3

[0051] 1. Same as implementation case 1;

[0052] 2. Take the Mg prepared in step 1 2 Al(OH) 6 (NO 3 )·6H 2 O hydrotalcite precursor 0.13g, add in the flask that 130ml formamide is housed, under N 2 Stirring and reacting for 50 hours under protection, centrifuging to obtain the hydrotalcite laminate colloid solution after peeling off;

[0053] 3. The preparation concentration is 3×10 -5 The aqueous solution of erythrosin B sodium salt of mol / L is adjusted to pH 7 with NaOH, and stored in a volumetric flask in the dark;

[0054] 4. Take a 3cm×1cm quartz plate and use concentrated H with a volume ratio of 7:3 2 SO 4 and H 2 o 2 Wash with the mixed solution of ethanol, and then ultrasonically use ethanol for 4 times, each time for 25 minutes, to obtain a quartz sheet rich in hydroxyl groups on the surface, and then place it in the hydrotalcite laminate colloid solution prepared in step 2 for 22 minutes for pre-assembly, take it out and use Rinse with deionized water and...

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Abstract

The invention belongs to the technical field of sensor manufacturing and in particular relates to a fluorescent sensor for determining serum protein concentration and a manufacturing method thereof. By utilizing a layer-by-layer assembly method of supermolecule, the invention compounds a positively charged nano hydrotalcite layer board with a negatively charged erythrosine guest molecule into a thin film for the determination of serum protein concentration, wherein the determining range is 5-90 ug / ml, the determining limit is 2.51ug / ml, and the determining time is 10-20 seconds. Compared with the traditional determining method, the sensor manufactured by the invention is high in serum protein selectivity in serum substances, short in determining time, wide in determining and applicable ranges, low in cost, simple and practical, and easy for realizing mass production. And the linear relation appears in a certain scope. During the determination, the systems to be determined are not polluted by the sensor and no reagent is lost. After the determination, the desorption for big molecule proteins of the sensor is realized by combining big molecule proteins through a sodium oleate competition, thereby realizing regeneration and reuse of the sensor.

Description

technical field [0001] The invention belongs to the technical field of sensor preparation, in particular to a fluorescent sensor for measuring serum protein concentration and a preparation method thereof. Background technique [0002] In the field of biological detection, the change of serum protein content is related to the protein loss caused by various causes such as hemodilution, malnutrition, chronic wasting disease, liver protein synthesis dysfunction, etc. hot topic of research. The conventional methods currently used in protein detection mainly include the following three: Kjeldahl method, biuret method, Lowry method, BCA method, colloidal gold method based on the composition characteristics of protein elements; Coomass method based on protein chemical color reaction. Brilliant blue method, silver staining method; UV spectrophotometry and fluorescence method based on the light absorption properties of proteins. Among these conventional methods, the fastest detectio...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 靳兰王腾利卫敏
Owner BEIJING UNIV OF CHEM TECH
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