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Composite nano-grade novel material based on cancer early-stage integrated detection, diagnoses, and treatment, and preparation method thereof

A composite nanomaterial and early detection technology, applied in the field of nanoscience, can solve the problems of low sensitivity and specificity, difficult detection, drug resistance, etc., and achieve the effects of high sensitivity and accuracy, improved accuracy and stable performance

Inactive Publication Date: 2015-01-28
SHENYANG POLYTECHNIC UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The sensitivity and specificity of the current conventional diagnostic methods are relatively low, it is difficult to detect in the early stage of tumor occurrence, there are also poor targeted delivery effect, strong toxic side effects and drug resistance of tumor cells, etc., it is difficult to achieve the expected therapeutic effect

Method used

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  • Composite nano-grade novel material based on cancer early-stage integrated detection, diagnoses, and treatment, and preparation method thereof
  • Composite nano-grade novel material based on cancer early-stage integrated detection, diagnoses, and treatment, and preparation method thereof
  • Composite nano-grade novel material based on cancer early-stage integrated detection, diagnoses, and treatment, and preparation method thereof

Examples

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preparation example Construction

[0041] A method for preparing a composite nano-material based on the integration of early detection, diagnosis and treatment of cancer as described above, characterized in that: the specific steps are as follows:

[0042] (1), using silver nanocube as template, adding AuCl 2 - and AuCl 4 - , to prepare hollow gold nanocubic box particles (nanoboxes) and hollow porous gold nanocage particles (nanocages), the hollow gold nanocubic box particles with H 2 o 2 Corrosion, get hollow gold nanocubic box-shaped particles with reduced angle, such as figure 1 What is not in the middle;

[0043] (2), add 0.02molFeCl to the hollow gold nano cube box-shaped particle and the hollow porous gold nano cage particle solution obtained in step (1) 2 ·6H 2 O dissolved in 200 mL deionized water and FeCl 3 4H 2 O, Fe 3+ with Fe 2+ The molar ratio is 1.75, the reaction precursor solution is stirred rapidly, and ultrasonically oscillated to ensure that the Fe 2+ and Fe 3+ The solution can ...

Embodiment 1

[0066] Take Au Nanocages / Fe 3 o 4 bioprobe

[0067] (1) Prepare nano-Fe with reference to the literature [Chinese Sci Bull, 2010, 55] 3 o 4 Particles, refer to literature [Adv Mater, 2008, 20] to prepare gold nanocages or gold nanocube boxes.

[0068] (2) Take (1) Au Nanocages particles 2g 10mmol, add 0.02mol FeCl 3 ·6H 2 O was dissolved in 200mL deionized water to form a 0.1mol / L solution, Fe 3+ with Fe 2+ The molar ratio is 1.75. When the reaction starts at 60°C, first add 30mL of ammonia water, and then add 0.5mL of surfactant when the temperature rises to 80°C. The aging time is 40-130min; the stirring speed is close to 300r / min, and composite nanoparticles are obtained. .

[0069] (3) At room temperature, add 1.5 mL of 20% PDDAC aqueous solution into 5 mL of deionized water and stir rapidly, then slowly add the 7 nM nano-Au Nanocages / Fe prepared in (2) 3 o 4 Solution 5mL, continue to stir for two hours, centrifuge at 15000rpm for 30 minutes, remove excess PDDAC ...

Embodiment 2

[0074] Synthesis of Nanocomposites Modified with 2,3-Dimercaptosuccinic Acid (DMSA)

[0075] (1) Reference [J Colloid Interface Sci.1997, 194:427-433] method, add 1mL DMSA solution (3mM) to 1.5mL nanocomposite colloid solution (7.0×10 12 particles / mL), stirred gently for 2 hours to obtain a DMSA-modified composite nanomaterial.

[0076] (2) (1) was centrifuged and washed with Milli-Q water to remove unreacted DMSA (3000 rpm). The nanomaterials were then redispersed in 3 mL of aqueous solution for later use.

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Abstract

The invention provides a composite nano-grade novel material based on cancer early-stage integrated detection, diagnoses, and treatment, and a preparation method thereof. According to the invention, gold nano-cage (gold nano-box) and nano-grade iron oxide are assembled, such that gold nano-cage (box) / nano-grade iron oxide composite particles are synthesized. With an assembling method from the inside to the outside, the gold nano-cage (box) is first prepared; iron oxide is nucleated and grown in the gold nano-cage (box); with a surfactant (such as glucan or the like), mono-dispersed iron oxide is obtained; the iron oxide is packaged by using biological molecules, such that the gold nano-cage (box) / nano-grade iron oxide composite particles are obtained; surface modification is carried out by using biological targeting molecules (antibodies or DNA molecules). The material is used in tumor magnetic resonance imaging, fluorescence imaging, dark field imaging, specific targeting multifunctional diagnosis, and photothermal therapy. The material can also be used as a targeting medicine carrier.

Description

Technical field: [0001] The invention mainly relates to a new composite material based on the integration of early detection, diagnosis and treatment of cancer - gold nanocage / nanometer iron oxide, which belongs to the field of nano science and technology. Background technique: [0002] Biologically active substances such as antibody proteins are difficult to quantitatively analyze or detect due to their relatively weak detectable signals. Cancer is a common and frequently-occurring disease that seriously endangers human health. According to the report of the World Health Organization, there are more than 10 million new cases of cancer worldwide each year, and more than 7 million deaths, including 5.3 million men and 4.7 million women. 12% of total deaths. It is expected that by 2020, there will be 15 million new cases worldwide each year. The total number of cancer patients will increase by 73% in developing countries and 29% in developed countries. Cancer is one of the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/06A61K41/00A61K47/02B82Y5/00B82Y40/00A61P35/00
Inventor 杨玉东
Owner SHENYANG POLYTECHNIC UNIV
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