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Method for detecting compound aminophenazone and barbital injection

A compound ammonium barbital and detection method technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of fast wear and tear of chromatographic columns, and achieve the effects of low cost, easy operation, and low equipment loss

Active Publication Date: 2013-06-12
SICHUAN KELUN PHARMA RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are also reports in the literature on the method of simultaneously determining the contents of the three components, but ion pairing reagents are required and the pH value is too high, which easily leads to rapid wear of the chromatographic column

Method used

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  • Method for detecting compound aminophenazone and barbital injection
  • Method for detecting compound aminophenazone and barbital injection
  • Method for detecting compound aminophenazone and barbital injection

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Experimental program
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Effect test

Embodiment 1

[0060] Embodiment 1 Detection method of the present invention

[0061] Instruments and conditions: Agilent1200 liquid chromatograph, DAD detector, chromatographic column: AgilentTC-C18 (250mm*4.6mm, 5um); detection wavelength: 220nm; column temperature: 30°C; buffered with 0.02mol / L potassium dihydrogen phosphate solution (pH adjusted to 5.5) - acetonitrile = 85:15 as the mobile phase.

[0062] Determination of mixed reference solution:

[0063] Take appropriate amounts of reference substances of aminopyrine, antipyrine and barbiturate respectively, add mobile phase to prepare solutions with concentrations of 0.05mg / ml, 0.02mg / ml, and 0.009mg / ml. Take another 1ml of compound ammonium barbiturate injection and place it in a 100ml measuring bottle, add mobile phase to constant volume, measure according to the law, see HPLC chart figure 1 .

[0064] Depend on figure 1 It can be seen that the peak retention times of barbiturate, antipyrine and aminopyrine are 7.801 minutes, 11.1...

Embodiment 2

[0075] The screening of embodiment 2 detection conditions of the present invention

[0076] 1 Determination of detection wavelength

[0077] Take appropriate amount of raw materials of aminopyrine, antipyrine and barbiturate respectively, and add mobile phase to prepare solutions with concentrations of 0.05mg / ml, 0.02mg / ml and 0.009mg / ml. Scanning in the wavelength range of 200-400nm, aminopyrine has maximum absorption at 233nm and 268nm, antipyrine has maximum absorption at 243nm and 261nm, barbiturate has absorption at 200-240nm and with the wavelength Increase and decrease, considering that the content of barbiturates in the three components is low, and at the same time, in order to avoid the interference of the mobile phase at a wavelength near 200nm, 220nm was selected as the detection wavelength of the test.

[0078] 2 Screening of mobile phase pH

[0079] Instruments and conditions: Agilent1200 liquid chromatograph, DAD detector, chromatographic column: AgilentTC-C18 ...

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Abstract

The invention discloses a method for detecting compound aminophenazone and barbital injection by means of high performance liquid chromatography. By virtue of the method, all effective components in the compound aminophenazone and barbital injection can be completely separated from impurity peaks, the content of each component in the injection can be determined correctly, the operation is simple and convenient without special flowing phase or chromatographic column, the equipment loss is small, the cost is low and a guarantee is provided for the injection drug safety.

Description

technical field [0001] The invention relates to a detection method for compound ammonium barbiturate injection. Background technique [0002] Compound aminopyrine-barbital injection is a compound preparation composed of aminopyrine 100mg, antipyrine 40mg, and barbiturate 18mg. Aminopyrine and antipyrine belong to pyrazolone antipyretic and analgesic drugs, which can inhibit the synthesis and release of hypothalamic prostaglandin, restore the normal reactivity of sensory neurons in the temperature regulation center and play an antipyretic effect; Inhibit the synthesis of prostaglandins and other analgesic effect. Aminopyrine can inhibit the synthesis and release of prostaglandins in local tissues of inflammation, stabilize the lysosome membrane, and affect the phagocytosis of phagocytes to play an anti-inflammatory effect. Combined use of barbiturates can enhance the analgesic effect. [0003] In 2003, the compound ammonium barbiturate injection was launched in the domesti...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 高国玉赵瀚晶王静周莉娅梁隆王利春沈鑫程志鹏
Owner SICHUAN KELUN PHARMA RES INST CO LTD
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