Method for detecting growth traits of cattle through assistance of LHX3 genes and special kit operational rule thereof

Abstract

The invention discloses a gene diagnostic kit for detecting growth traits of cattle, and a method for detecting the growth traits of the cattle through LHX3 genes. The method includes that whole genome deoxyribonucleic acid (DNA) of to-be-detected cattle serves as a template, the whole genome DNA comprises the LHX3 genes, a pair of primers (P) serves as a primer, a polymerase chain reaction (PCR) amplifies the LHX3 genes of the cattle, and detection of gene polymorphism and quick parting are conducted through DNA sequencing and a PCR-single strand conformation polymorphism (SSCP) technology. The method includes that molecular genetic markers which are closely related to growth traits of Qinchuan cattle are screened and detected in the DNA standard, so that the detection method can be used for table purposes and assistant selection of the molecular markers of the growth traits of the Qinchuan cattle, and accordingly a cattle population with good genetic resources is built quickly. The detection method is simple, cost is low, detection results are direct and reliable, and the method is suitable for large-scale screening and diagnosis of a 10385th mutation of the LHX3 genes.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to the detection of gene single nucleotide polymorphism, in particular to a method for assisting the detection of growth traits of cattle by LHX3 gene and the operating rules of the special kit. Background technique [0002] Single nucleotide polymorphism (single nucleotide polymorphism, SNP) is a type of genetic marker system proposed by Lander (1996), a scholar at the Human Genome Research Center of the Massachusetts Institute of Technology. Polymorphism caused by changes in acid (A / G / C / T). Its variant forms include: transversion, conversion, insertion and deletion, etc., which are mainly caused by the conversion or transversion of a single base. SNPs with transformational base variation accounted for about 2 / 3. Various numbers of SNPs may be found in or near any known or unknown gene. There are relatively few SNPs (cSNPs) in the gene coding region, because the variation rate in exons ...

AI Technical Summary

Problems solved by technology

Mutations in the transcription factor LHX3 can affect the development of the pituitary gland, causing a deficiency of pituitary hormones such as GH

Studies have revealed that the point mutation of the LHX3 gene in humans leads to impaired regulation of the gene's activation and regulation of pituitary genes, thereby causing disorders of pituitary hormone secretion

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