Method for detecting growth traits of cattle through paired box gene 6 (PAX6) and diagnostic kit thereof

A gene and genotype technology, applied in the fields of biochemical equipment and methods, determination/inspection of microorganisms, etc.

Inactive Publication Date: 2013-06-12
NORTHWEST A & F UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] At present, there are very few data reports on the study of the PAX6 gene in domestic livestock, and there is no report on the polymorphism of this gene in the cattle as a large livestock in China

Method used

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  • Method for detecting growth traits of cattle through paired box gene 6 (PAX6) and diagnostic kit thereof
  • Method for detecting growth traits of cattle through paired box gene 6 (PAX6) and diagnostic kit thereof
  • Method for detecting growth traits of cattle through paired box gene 6 (PAX6) and diagnostic kit thereof

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Embodiment Construction

[0052] In the present invention, primers are firstly designed according to the PAX6 gene sequence (GenBankAccession No. NW001493316.1) published by NCBI, and the genomic DNAs of three cattle breeds are used as templates to carry out PCR amplification, and the PCR products are mixed, purified, and sequenced. Then, carry out sequencing map analysis and sequence comparison to screen out SNP sites; secondly, carry out PCR-SSCP detection of polymorphic sites in the tested population; finally, carry out statistical analysis of population genetics according to the genotypes detected in the population The association analysis with growth traits screened out the molecular markers closely related to the growth traits of cattle. The present invention will be described in detail below, which is an explanation of the present invention rather than a limitation.

[0053] Cloning of Partial DNA Sequence of PAX6 Gene of I Local Yellow Cattle Breed

[0054] 1. Cattle sample collection

[0055...

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Abstract

The invention discloses a gene diagnostic kit for detecting growth traits of cattle, and a method for detecting the growth traits of the cattle through a paired box gene 6 (PAX6). The method comprises that whole genome deoxyribonucleic acid (DNA) of to-be-detected cattle serves as a template, the whole genome DNA comprises the PAX6, a pair of primers (P) serves as a primer, a polymerase chain reaction (PCR) amplifies the PAX6 of the cattle, and detection of gene polymorphism and quick parting are conducted through DNA sequencing and a PCR-single strand conformation polymorphism (SSCP) technology. The method includes that molecular genetic markers which are closely related to the growth traits of the cattle are screened and detected in the DNA standard, so that the detection method can be used for assistant selection of the molecular markers of the growth traits of the cattle, and accordingly a cattle population with good genetic resources is built quickly. The detection method is simple, cost is low, detection results are direct and reliable, and the method is suitable for large-scale screening and diagnosis of a 17056th mutation of the PAX6 of the cattle.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to the detection of gene single nucleotide polymorphisms, in particular to a method for detecting growth traits of cattle with PAX6 gene and a diagnostic kit thereof. Background technique [0002] A genetic marker refers to any genetic trait that can trace a chromosome, a segment of a chromosome, or a locus that is transmitted in a family. It has two basic characteristics, that is, heritability and identifiability, so any genetic mutants with different phenotypes can be used as genetic markers. Genetic markers have roughly gone through four stages of development: morphological markers, cell markers, biochemical markers and molecular markers. In 1974, Grodzicker et al. proposed for the first time to use restriction fragment length polymorphism (RFLP) as a genetic marker, creating a precedent for directly using DNA as a genetic marker, thus unveiling the prelude to the study of molecular ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 陈宏黄永震魏天宝蓝贤勇雷初朝胡沈荣
Owner NORTHWEST A & F UNIV
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