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Production method for fuel ethanol

A production method and technology for fuel ethanol, which are applied in microorganism-based methods, biochemical equipment and methods, biofuels, etc., can solve the problem of low ethanol concentration, and achieve the goal of improving ethanol yield, shortening process flow, and improving production efficiency. Effect

Active Publication Date: 2013-05-15
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problem that the existing fiber ethanol technology needs to develop special equipment for high dry matter concentration system hydrolysis and the ethanol concentration in the fermentation liquid is not high, the present invention adjusts the raw material structure, adds cheap carbon source raw materials and synchronizes saccharification and fermentation. , increase the ethanol concentration in the fermentation broth of fiber raw materials, and reduce the energy consumption in the stage of ethanol concentration

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 (comparative scheme) separate fermentation of corn stalks

[0027] Add 2 L of tap water, 0.4 kg of steam explosion pretreated corn stalks, and 50 g of yeast powder into a 5 L fermenter to adjust the pH to 5.0. At the same time, 800 mL of YPD culture was prepared in a 3 L Erlenmeyer flask, sterilized, inoculated with FE-B yeast, and cultured overnight.

[0028] Add 200 mL of cellulase to a 5 L fermenter, and insert 800 mL of yeast cultured overnight. Simultaneous saccharification and fermentation were carried out at 42°C. At 24h, 48h, 72h and 96h, 0.2 kg of steam explosion pretreated corn stalks were added, and the ethanol concentration in the system was 8.2 wt% (weight percentage) after 120 hours.

Embodiment 2

[0029] Embodiment 2 (comparative scheme) acorn is fermented separately

[0030] Take 1.2 kg of acorn powder and add 3L of tap water into a double-layer glass reactor (5L), use the interlayer to keep the temperature of the system at 50°C, extract for 24 hours, and centrifuge. The solid residue is washed once with an equal volume of hot water, and then Centrifuge. The washed solid slag was mixed with water below 50°C to prepare an emulsion, the solid content of the emulsion was 34wt%, and a certain amount of 10wt% dilute lye was added under constant stirring to make the pH of the emulsion reach 5.8. Add Novozymes Liquozyme Supra / 2.2x Amylase 0.464 g. Liquefied at 95°C for 1 hour, the measured DX value of the liquefied liquid was 15%. Then lower the temperature, adjust the pH to 4.5, add 0.387 g of Suhong GA 475 glucoamylase from Novozymes, and saccharify at 58°C for 48 h. The DX value in the saccharified feed solution is measured to be 65%. Then, 0.05% dry weight yeast was...

Embodiment 3

[0031] Example 3 (comparative scheme) co-fermentation of corn stalks and acorn powder

[0032] A double-layer glass reactor (10 L) was added with 1.2 kg of acorn powder and 1 kg of steam explosion pretreated corn stalk, 5.5 L of tap water, and a certain amount of 10wt% dilute lye was added under constant stirring to make the pH of the emulsion reach 5.8. Add Novozymes Liquozyme Supra / 2.2x Amylase 0.464 g. Liquefy at 95°C for 1 hour, then lower the temperature, adjust the pH to 4.5, pour into a 10L fermenter, add 50 g of yeast powder, 200 mL of cellulase and 0.387 g of Novozymes’ Suhong GA 475 starch glucoamylase at 50°C for enzymatic hydrolysis 48h. At the same time, 800 mL of YPD culture was prepared in a 3 L Erlenmeyer flask, sterilized, inoculated with FE-B yeast, cultured overnight, and put into the above-mentioned 10L fermenter as seeds, cultured at 36 °C for 24 h, and then cultured at 42 °C for 48 h. The ethanol concentration in the fermentation broth was 8.5 wt%. ...

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Abstract

The invention relates to a production method for fuel ethanol. The method comprises the following steps: (1) pretreating a fiber raw material and a cheap carbon source raw material; (2) mixing the pretreated fiber raw material and the pretreated cheap carbon source raw material and carrying out pre-enzymatic hydrolysis, wherein a weight ratio of the cheap carbon source raw material to the fiber raw material is 1: 3 to 3: 1 on a dry basis, an obligate hydrolase preparation is used in the process of pre-enzymatic hydrolysis, and the obligate hydrolase preparation contains cellulase and comprises diastase and / or inulase at the same time; and (3) selectively adding a fermentable sugar raw material in the fiber raw material having undergone pre-enzymatic hydrolysis, adjusting a temperature to 24 to 44 DEG C, adding saccharomyces cerevisiae and carrying out synchronous saccharification and joint fermentation for production of ethanol. The method provided by the invention can effectively increase the concentration of ethanol in the fermentation broth of the fiber raw material and reduce energy consumption in the phase of concentration of ethanol and has the advantages of simple production flow and high ethanol yield.

Description

technical field [0001] The invention relates to a production method of fuel ethanol, in particular to a method for increasing the concentration of ethanol in fermentation liquid of cellulosic raw materials. Background technique [0002] As the world's population grows and countries become more industrialized, energy consumption continues to rise. Oil is the main resource to meet energy demand, but oil resources are limited. Scientists predict that crude oil production will drop from 25.8 billion barrels (3.525 billion tons) in 2009 to 5 billion barrels by 2050 (Campbell C J, Laherrere J H. The end of cheap oil. Sci Am, 1998, 3: 78-83; Fengfeng. Dynamics of world oil and gas resources reserves and production. China Petroleum and Chemical Industry, 2010, 04:14-15). As a renewable transportation fuel, bioenergy can effectively reduce the room temperature effect, slow down environmental pollution, and at the same time change the existing unbalanced oil supply and demand relatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/10C12P19/14C12R1/865
CPCY02E50/16Y02E50/10
Inventor 张全黎元生关浩佟明友金平
Owner CHINA PETROLEUM & CHEM CORP
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