Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Whole genome DNA of scylla paramamosain mitochondria and testing method

A mitochondrial gene, whole genome technology, applied in genetic engineering, plant genetic improvement, botanical equipment and methods, etc., can solve the problems of low genetic information and misleading research results, and achieve the effect of low cost and easy to master

Inactive Publication Date: 2013-04-24
EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
View PDF2 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the genetic information revealed by single-gene markers is much lower than that provided by whole genomes and has the potential to mislead research results

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Whole genome DNA of scylla paramamosain mitochondria and testing method
  • Whole genome DNA of scylla paramamosain mitochondria and testing method
  • Whole genome DNA of scylla paramamosain mitochondria and testing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Collection and analysis of mitochondrial-related gene sequences of Scylla pseudocavena

[0024] First, on the one hand, the present invention screens the mitochondrial-related gene sequences of Scylla pseudocarpus from the transcriptome library obtained by sequencing; The mitochondrial genome sequences of closely related species Scylla serrata and Portunus trituberculatus were compared, and the relative positions of related genes in the mitochondrial genome sequences of closely related species were analyzed.

[0025] 2. Primer design and PCR amplification

[0026] According to the relative position of the mitochondrial gene in the whole genome sequence of the closely related species, the primers were designed using the Scylla pseudosatis gene sequence as a template, and the unknown sequence between two adjacent genes was amplified by PCR method. The PCR amplification reaction system is 25 μl, including 1 μl of genomic DNA template, upstream and downstream primers wi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a whole genome DNA of scylla paramamosain mitochondria and a testing method. The DNA molecule which is in a closed ring structure has a length of 15824 bp; and the testing method comprises the steps of: (1) collecting relative gene sequences of the scylla paramamosain mitochondria and comparing the relative gene sequences of the scylla paramamosain mitochondria with whole genome sequences of mitochondria of sibling species; (2) designing a primer and carrying out PCR (Polymerase Chain Reaction) amplification; and (3) sequencing PCR products and assembling the sequences. According to the invention, the whole genome DNA sequences of the scylla paramamosain mitochondria are obtained. The testing method disclosed by the invention has the advantages of being fast in speed, low in cost, easy to master and the like; and the obtained whole genome DNA of the mitochondria can be applied to the fields such as heritable variation analysis of the scylla paramamosain, genetic resource authorization and protection, phylogeny and the like.

Description

technical field [0001] The invention belongs to the field of Scylla pseudomae genomics, and in particular relates to a whole genome DNA of the Scylla pseudomae mitochondria and a detection method. Background technique [0002] In most multicellular animals, mitochondrial genomic DNA is a typical closed circular structure with a length between 14 and 18 kb, capable of autonomous replication and transcription. The mitochondrial genome usually encodes 37 genes, including: 13 protein-coding genes, 22 tRNA genes, and 2 rRNA genes. In addition, it also contains a large non-coding region (control region). The whole mitochondrial genome can not only provide more genetic information than a single gene, but also reveal the characteristics of the genome level, which helps to better understand the characteristics of genome evolution and development. So far, scholars have revealed the whole mitochondrial genome DNA information of many crustaceans, including: Penaeus monodon, Penaeus chi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C12Q1/68
Inventor 马洪雨马凌波马春艳
Owner EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com